Indian Journal of Biochemistry and Biophysics


The journal welcomes concise reports of original research in all areas of biochemistry and biophysics. Papers on nutrition or medical biochemistry will be considered only if they are of general biochemical interest.



Hard copies of manuscripts (inclusive of illustrations) should be submitted in triplicate to the Editor, Indian Journal of Biochemistry and Biophysics, National Institute of Science Communication and Information Resources (NISCAIR), Dr K S Krishnan Marg, New Delhi 110012. Submission of a manuscript to this journal implies that it is not under consideration for publication elsewhere.


Manuscripts should be presented in as concise a form as possible and typewritten in double space on one side of the paper. Pages should be numbered consecutively, and the matter on Page 1 should be arranged in the following order: Title of the paper; Name(s) of Authors(s), Department(s) and Institution(s); Foot Note containing address of Author for correspondence with e-mail id, telephone no., FAX no. followed by list of Abbreviations used in text. A short running title derived from the original title may also be given. Page 2 should contain the Abstract. Rest of the matter may be arranged in the following order:


Introduction; Materials and Methods; Results; Discussion; Acknowledgement; References. Abstract, Tables and Captions for Figures should be typed separately.

Title: The title should be such as to be useful in indexing and information retrieval. If a paper forms part of a series, a sub-title indicating the aspects of the work covered in the paper should be provided.

Abstract: The abstract, not exceeding 200 words, should indicate the scope and significant content of the paper, highlighting the principal findings and conclusions. Keywords are to be given beneath the abstract.

Introduction: The introductory part should bear no heading, should be brief and state precisely the objective in relation to the present status of knowledge in the field. Reviewing of the literature should be restricted to only essential background and the reason for the research undertaken.

Materials and Methods: The nomenclature, the sources of materials and the procedures should be clearly stated. New methods should be described in sufficient detail, but if the methods are already well known, a mere reference to them will do; deviations, if any, should, however, be given.

Results: Only such data as are essential for understanding the discussion and main conclusions emerging from the study should be included. The data should be arranged in a unified and coherent sequence for clarity and readability. The same data should not be presented in both tabular and graphic forms. Only such tables and figures as are necessary should be given. Lineweaver-Burk plots, pH curves, substrate and enzyme concentration curves, and gel filtration and gel electrophoresis calibration curves for molecular weights of enzymes will be published only if they are non-standard.

In studies dealing with experimental animals, tests of statistical significance should be identified and references used should be cited. Statements about the statistical significance of the results should be accompanied by indications of the level of significance, preferably provided in respective Tables and Figure legends.

Discussion: Long rambling discussion must be avoided. The discussion should deal with the interpretation of results without repeating information already presented under Results. It should relate the new findings to the known and include logical deductions. In some cases, however, it may be desirable to combine results and discussion in a single section.

Illustrations: The number of illustrations should be kept to the minimum and numbered consecutively in Arabic numerals.. Simple linear plots or linear double reciprocal plots that can be easily described in the text should be avoided. Extension of graphs beyond the last experimental point is permissible only while extrapolating.

Captions and legends to the Figures should be self-explanatory and typed on a separate sheet of paper. Line drawing should be either laser prints of computer generated illustrations or manually made in Indian ink on white drawing paper, and should be drawn to approximately twice the printed size. The drawings are reduced to the page (width, 175 mm) or column (width, 85 mm) size, and care should be taken that the size of letters, numerals, dots and symbols is relatively uniform and sufficiently large to permit this reduction.

Tables: Each table should have an explanatory title and should be numbered in Arabic numeral. It should have sufficient experimental details, usually in the form of a paragraph immediately following the title. Units of measurement should be abbreviated and placed below the headings. Negative results should be indicated as ‘Nil’ and absence of a datum by a dash. Presentation of results should be limited to the accuracy of the method employed.

Acknowledgement: Acknowledgement should be brief and for especial assistance only, not for providing encouragement and routine facilities et cetera.

References: Responsibility for the accuracy of bibliographic references rests with the author. Abstracts of papers presented at scientific meetings should be cited only after they appear in publications included in the Biological Abstracts. Such Abstracts may be cited in Foot Note. The Indian Journal of Biochemistry and Biophysics has accepted the recommendations of the Commission of Editors of Biochemical Journals of the International Union of Biochemistry, concerning presentation of reference lists. The recommended style is as follows:



Upadhyay S N & Chattoraj D K (1972) Indian J Biochem Biophys 9, 17-20


In case of minireview, title of the papers may be included.



Dixon M & Webb E C (1964) Enzymes, 2nd edn, pp. 565-567, Longmans Green, London

Bracket J (1967) in Comprehensive Biochemistry (Florkin M & Stotz E M, eds), Vol. 28, pp. 23-54, Elsevier, Amsterdam

Laidler K J & Bunting P S (1973) The Chemical Kinetics of Enzyme Action, 2nd edn, Vol. 2, Chapt. 4, pp. 255-278, Clarendon Press, Oxford


Reports, non-serial symposia & proceedings

Harding B W, Whysner J, Cheng S C & Ramseyar J (1970) Proceedings of the Third International Congress, Hormonal Steroids, Hamburg, September 1970, pp. 294-300

Technicon Autoanalyzer Methodology (1971) method file N-24, pp. 1-4

Iverson H O (1969) Homeostatic Regulators (Ciba Foundations Symposium), pp. 29-30, J & A Churchill Ltd, London



Chandrasekharan K S (1956) Studies on Crystal Structure and Absolute Configuration of Crystals, Ph. D. thesis, Madras University, Madras.

References should be cited in the text by number, and the reference list should be in the order of citation, not in alphabetical order. The first and last page numbers are to be included.

Unpublished work should normally be avoided. If necessary it may not be listed in the references, but mentioned in the text as, for example, (Pande, A B, unpublished data).

Reference to patent should include the names of patentees, the country of origin (underlined) and the patent number, the organization to which the patent has been assigned (within circular brackets), the date of acceptance of the patent and the reference to an abstracting periodical where available [e.g. Trepagnier, J H, US Pat, 2,463,219 (to E I du Pont de Nemours & Co.), 1 March 1949; Chem Abstr, 43 (1949), 7258].

Nomenclature and Units: Standard terminology for biochemical compounds and uniform units in biochemistry and molecular biology recommended by IUPAC-IUBMB joint committee should be used. (The website,, contains updates and links to internationally agreed recommendations of use to biochemists).

Enzyme Nomenclature: For enzymes, only the trivial names recommended by the IUB-IUPAC Commission on Enzyme Nomenclature, 1992 (Academic Press, 1250, Sixth Avenue, San Diego, California 92101- 4331, USA) should be used. In some cases, where the enzyme is the main subject of a paper, its code number and systematic name should also be stated at its first citation in the paper.

Spectrophotometric Data: While reporting spectrophotometric data, the relation between the symbols used must be indicated. It is suggested that the symbols and terminology adopted by IUPAC [(1970) {Pure Appl. Chem. 21, 1] may be adhered to. Beer’s law may be stated as


        A = - log10 T = elc


where A is the absorbance; T, the transmittance (= I/I0); e, the molar absorption coefficient; c, the concentration of the absorbing substances in moles per litre; and l, the length of the optical path in centimeters.

Molecular weight being ratio is a pure number without any unit. It should be expressed as the relative moleclular mass (Mr).

Molecular mass (m) in contrast, is not a ratio, and can be expressed in daltons (Da). Molecular mass is the mass of one molecule of a substance.

Abbreviations: Symbols and abbreviations should conform to the recommendation of the Commission on Biochemical Nomenclature (CBN) of IUPAC-IUB as approved by the Commission of Editors of Biochemical Journals. Non-standard abbreviation, when used, must be defined in a footnote to the first abbreviation. Generic terms (virus, protein etc.), short trivial names (folate, adenosine, glycerol etc.) and enzyme names should not be abbreviated. However, an accepted abbreviation for the substrate may be incorporated in the enzyme name (e.g. glucose-6-P dehydrogenase, ATPase, but glucose 6-phosphatase, glutamate dehydrogenase). RNase, for ribonuclease, and DNase, for deoxyribonuclease, are permitted exceptions to this rule.

Authors are advised to see a recent issue of the journal to get familiar with the format and the practices adopted in respect of various elements of a paper.


Accepted abbreviations: The following abbreviations can be used without definition:


5¢-Pyrophosphates of adenosine, cytidine, guanosine, inosine, uridine and xanthosine.


Adenosine 5¢-phosphate


Adenosine 5¢-triphosphate


Flavin mononucleotide


Glutathione, reduced and oxidized

CoA. Acyl-Coa

Coenzyme A and its acyl derivative


Nicotinamide adenine dinucleotide, its¢ Oxidised and Reduced forms


Nicotinamide adenine dinucleotide phosphate, its Oxidised and Reduced forms


Nicotinamide mononucleotide

Pi, PPi

Orthophosphate, pyrophosphate


Ubiquinone, Ubisemiquinone, Ubiquinol


Ribosylthymine 5¢-diphosphate


Ribosylthymine 5¢-phosphate



Uridine diphosphate glucose


Uridine 5¢-phosphate


Uridine 5¢-triphosphate


Ribosomal RNA


Nuclear RNA


Messenger RNA


Transfer RNA


Complementary RNA


Mitochondrial RNA


Heterogeneous nuclear RNA


Tris (hydroxymethyl) aminomethane



CM cellulose

Carboxymethyl cellulose






Proofs and Reprints: Gallely proofs are not sent to authors except to those who make specific request for the same. Authors should ensure that the data submitted for publication are error-free. Twenty five reprints, without cover, are supplied free of charges to the communicating author.