Indian Journal of Biochemistry 
& Biophysics  

Total visitors: 2,340  since 09-12-05

 

ISSN : 0301-1208

CODEN : IJBBBQ  

VOLUME 42

NUMBER 6

DECEMBER 2005

 

 

CONTENTS

 

Papers

 

Overexpression and characterization of a novel chitinase gene from a marine bacterium Pseudomonas sp. BK1 

339

      Moon-Sun Jang, Young-Mi Lee, Young-Su Cho, Yong-Lark Choi, Cherol-Ho Kim and Young-Choon Lee*

 

 

 

Dietary restriction and triiodothyronine (T3) regulation of malate-aspartate shuttle enzymes in the liver and kidney of mice

345

      Danswrang Goyary and R Sharma*

 

 

 

Production of human single-chain variable fragment (scFv) antibody specific for digoxin by ribosome display

350

      Xiang-Hua Yan and Zi-Rong Xu*

 

 

 

Correlation of HER1/EGFR expression and degree of radiosensitizing effect of the HER1/EGFR-tyrosine kinase inhibitor erlotinib

358

      Jae-Chul Kim, M Aktar Ali, Animesh Nandi, Partha Mukhopadhyay,  Hak Choy, Carolyn Cao and Debabrata Saha*

 

 

 

Expression of DΉ-pyrroline-5-carboxylate synthetase gene during drought in rice

      (Oryza sativa L.)

366

      N L Choudhary, R K Sairam and A Tyagi*

 

 

 

Induction of resistance in host against the infection of leaf blight pathogen

      (Alternaria palandui) in onion (Allium cepa var aggregatum)

371

      M Karthikeyan*, V Jayakumar, K Radhika, R Bhaskaran, R Velazhahan and D Alice

 

 

 

Energy barriers and rates of tautomeric transitions in DNA bases: Ab initio quantum chemical study

378

      Soumalee Basu, Rabi Majumdar, Gourab K Das and Dhananjay Bhattacharyya*

 

 

 

Reaction of substituted pyrimidines with photochemically generated t-BuO• radicals

386

      L Charitha and M Adinarayana*

 

 

 

Notes

 

Determination of serum glucose using co-immobilized glucose oxidase and peroxidase on to arylamine glass beads affixed on a plastic strip

391

      Neelima Tank, Suman and C S Pundir*

 

Antibacterial activity of crude methanolic extract and its fractions of aerial parts of Anthemis tinctoria

395

      Cahit Akgul* and Gulsen Saglikoglu

 

 

 

Antibacterial activity of some unsymmetrical diorganyltellurium(IV) dichlorides

398

      Daya Soni, Prabhat K Gupta*, Yatindra Kumar and T G Chandrashekhar

 

 

 

Lead levels in some biological samples of auto-mechanics in Abeokuta, Nigeria

401

      O O Babalola*, L O Ojo and M O Aderemi

 

 

 

Annual Author Index

404

 

 

Annual Subject Index

406

 

 

List of Referees

420

 

 

Instructions to Authors

423

 

 

——————

*Author for correspondence

 

 


AUTHOR INDEX

 

Cho Young-Su

339

Choi Yong-Lark

339

Jang Moon-Sun

339

Kim Cherol-Ho

339

Lee Young-Mi

339

Lee Young-Choon

339

Goyary D

345

Sharma R

345

Xu Zi-Rong

350

Yan Xiang-Hua

350

Ali M A

358

Cao C

358

Choy H

358

Kim Jae-Chul

358

Mukhopadhyay P

358

Nandi A

358

Saha D

358

Choudhary N L

366

Sairam R K

366

Tyagi A

366

Alice D

371

Bhaskaran R

371

Jayakumar V

371

Karthikeyan M 

371

Radhika K

371

Velazhahan R

371

Basu S

378

Bhattacharyya D

378

Das G K

378

Majumdar R

378

Adinarayana M

386

Charitha L

386

Pundir C S

391

Suman

391

Tank N

391

Akgul C

395

Saglikoglu G

395

Chandrashekhar T G

398

Gupta P K

398

Kumar Y

398

Soni D

398

Aderemi M O

401

Babalola O O

401

Ojo L O

401


 

 

 

 

Papers

 

Indian Journal of Biochemistry & Biophysics

Vol. 42, December 2005, pp. 339-344

 

Overexpression and characterization of a novel chitinase gene from a marine bacterium Pseudomonas sp. BK1 

Moon-Sun Jang, Young-Mi Lee, Young-Su Cho, Yong-Lark Choi, Cherol-Ho Kim1 and Young-Choon Lee*

 

The chitinase A (ChiA)-coding gene of Pseudomonas sp. BK1, which was isolated from a marine red alga Porphyra dentata, was cloned and expressed in Escherichia coli. The structural gene consists of 1602 bp encoding a protein of 534 amino acids, with a predicted molecular weight of 55,370 Da. The deduced amino acid sequence of ChiA showed low identity (less than 32%) with other bacterial chitinases. The ChiA was composed of multiple domains, unlike the arrangement of domains in other bacterial chitinases. Recombinant ChiA overproduced as inclusion bodies was solubilized in the presence of 8 M urea, purified in a urea-denatured form and re-folded by removing urea. The purified enzyme showed maximum activity at pH 5.0 and 40°C. It exhibited high activity towards glycol chitosan and glycol chitin, and lower activity towards colloidal chitin. The enzyme hydrolyzed the oligosaccharides from (GlcNAc)4 to (GlcNAc)6, but not GlcNAc to (GlcNAc)3. The results suggest that the ChiA is a novel enzyme, with different domain structure and action mode from bacterial family 18 chitinases.

Keywords: Pseudomonas, chitinase, cloning, overexpression, refolding

 

IPC Code: C12N15/52; C12N9/28

          E -mail:  yclee@dau.ac.kr

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 42, December 2005, pp. 345-349

 

Dietary restriction and triiodothyronine (T3) regulation of malate-aspartate shuttle enzymes in the liver and kidney of mice

Danswrang Goyary and R Sharma*

 

The activities of malate-aspartate shuttle enzymes viz., cytosolic and mitochondrial aspartate aminotransferase (c- and m-AsAT) and malate dehydrogenase (c- and m-MDH) were measured in liver and kidney of ad libitum (AL) and dietary- restricted (DR) mice and also on triiodothyronine (T3) treatment. The results show that the activity (U/mg protein) of c-AsAT is increased significantly in liver and the activities of c-MDH and m-AsAT are increased significantly in kidney during DR. On T3 treatment, the activities of both the isoenzymes (c- and m-) of MDH and AsAT are increased significantly in the liver of AL- and DR-fed mice. In the kidney, m-MDH showed no effect by T3 treatment, however, c-MDH increased significantly in both AL- and DR-fed mice. In contrast, m-AsAT is increased significantly in the kidney in AL-fed mice, but was not affected in DR-fed animals. In vitro reconstitution of malate-aspartate shuttle showed a higher activity in the liver and kidney of DR-fed mice, as compared to AL-fed ones and also in the T3-treated mice, compared to untreated ones. These findings suggest that malate-aspartate shuttle enzymes are differentially regulated during DR in mice, in order to adapt to the metabolic need of liver and kidney. T3 potentially regulates the shuttle enzymes, albeit to a varying degree in the liver and kidney of AL- and DR-fed mice.

Keywords: Malate-aspartate shuttle enzymes, dietary restriction, triiodothyronine (T3), liver, kidney, mice.

          E-Mail: rsharma@nehu.ac.in

 

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 42, December 2005, pp. 350-357

 

Production of human single-chain variable fragment (scFv) antibody specific for digoxin by ribosome display

Xiang-Hua Yan and Zi-Rong Xu *

 

Ribosome display was applied in vitro to select single-chain variable fragment (scFv) antibody specific for digoxin from a human non-immune naive scFv library. A cell-free system was used to produce stable antibody-ribosome-mRNA (ARM) complexes to provide the linkage of genotype and phenotype, allowing simultaneous selection of a desired antibody together with its encoding mRNA. The mRNA was then recovered and amplified as DNA by reverse transcriptase-polymerase chain reaction (RT-PCR). Repeating the display cycle enriched the selected molecules, enabling rare species to be isolated. In this study, digoxin-binding segments were selected over four cycles of ARM display and the selected DNA was cloned and expressed as a single-chain variable fragment antibody (the best scFv, A3) in Escherichia coli. The affinity (equilibrium dissociation constant Kd) of digoxin was 8.3 Χ 10-8 M for A3, which validated construction of the naοve library and the power of ribosome display lending to the evolution of functional characteristics, such as potency of leading candidate antibodies to provide therapeutic antibodies. A3 was purified using affinity chromatography and determined by Western blot. The results indicate that ribosome display technnique can be efficiently used to isolate specific antibody fragments from a naive library.

Keywords: Single-chain Fv, ribosome display, non-immune libraries, digoxin

IPC Code: C07J19/00

          E-Mail: xhyan@zju.edu.cn; yanxiangh@sina.com

 

 

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 42, December 2005, pp. 358-365

 

Correlation of HER1/EGFR expression and degree of radiosensitizing effect of the HER1/EGFR-tyrosine kinase inhibitor erlotinib

Jae-Chul Kim¨, M Aktar Ali, Animesh Nandi, Partha Mukhopadhyay, Hak Choy, Carolyn Cao and Debabrata Saha*

 

Epidermal growth factor receptor (HER1/EGFR)-mediated signal transduction pathways are important in cellular response to ionizing radiation. High HER1/EGFR expression on cancer cells may contribute to radioresistance. In this pre-clinical study, we evaluated the radiosensitizing effect of erlotinib, a small molecule HER1/EGFR inhibitor in three human cancer cell lines with different HER1/EGFR expression — A431 (very high expression), H157 (moderate expression) and H460 (low expression). Our results demonstrated that A431 was the most radioresistant, while H460 was the most radiosensitive. However, A431 cells were the most sensitive to erlotinib (IC50 = 300 nM) and H460 cells the most resistant (IC50 = 8 mM). H157 had intermediate sensitivity to radiation and erlotinib (IC50 = 3 mM). With 300 nM erlotinib, the radiation dose enhancement ratios (DER) were 1.40, 1.17 and 1.04 in A431, H157 and H460, respectively. Treatment with erlotinib for 24 hr at 300 nM increased G1 arrest by 18.6, 2.0 and 4.8% in A431, H157 and H460, respectively. Erlotinib-induced apoptosis was augmented by radiation in A431 cells only. In conclusion, high HER1/EGFR expression may result in a high degree of radiosensitization with erlotinib combined with radiation. The extent of erlotinib-induced radiosensitization was proportional to HER1/EGFR expression, as well as autophosphorylation of the human epidermal growth factor receptor (HER1/EGFR).

Keywords: Epidermal growth factor (EGF), erlotinib, HER1/EGFR inhibitor, HER1/EGFR expression, radiosensitization, autophosphorylation, lung cancer, cell lines A431, H157 and H460, flow cytometric analysis, cell cycle analysis, apoptosis, tyrosine kinase activity

IPC Code: C12N 15/52

         E-Mail:  debabrata.saha@utsouthwestern.edu

 

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 42, December 2005, pp. 366-370

 

Expression of DΉ-pyrroline-5-carboxylate synthetase gene during drought in rice (Oryza sativa L.)

N L Choudhary1, R K Sairam2 and A Tyagi1*

 

The 45-days-old seedlings of drought resistant (N-22, CR143-2-2) and susceptible rice (Oryza sativa L.) genotypes (Panidhan, Pusa-169) were subjected to osmotic stress in PEG-6000 solution of -10 and -16 bar and the relative water content (RWC), proline content, and pyrroline-5-carboxylate synthetase (P5CS) activity and its P5CS expression were studied. A gradual decrease in RWC was observed in tolerant genotypes, whereas the decrease was drastic in susceptible ones. Proline content and P5CS activity increased both in susceptible and tolerant genotypes; the increase was higher in tolerant genotypes. Higher proline levels in tolerant genotypes were due to increased P5CS activity. The EcoRI, BamHI and XbaI restricted DNA of N-22 and Panidhan genotypes were hybridized with Arabidopsis P5CS sequence and a single band (approx 2.4 kb) was observed, however, P5CS expression was more in N-22, as compared to Panidhan.

Keywords: Rice, Oryza sativa L., osmotic stress, proline, pyrroline-5-carboxylate synthetase, P5CS expression,
relative water content

IPC Code: C12Q1/00, C12N15/52

          E-Mail: at_bio@iari.res.in

 

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 42, December 2005, pp. 371-377

 

Induction of resistance in host against the infection of leaf blight pathogen (Alternaria palandui) in onion (Allium cepa var aggregatum)

M Karthikeyan*, V Jayakumar1, K Radhika2, R Bhaskaran3, R Velazhahan and D Alice

 

The Pseudomonas fluorescens isolate Pf1 was found to inhibit the growth of pathogen Alternaria palandui, in vitro. In the present study, foliar application of a talc-based formulation of Pf1 significantly reduced the incidence of leaf blight of onion, caused by A. palandui. Induction of defense-related proteins viz., chitinase, b-1,3 glucanase, peroxidase (PO) and polyphenol oxidase (PPO) by application of Pf1, was studied against A. palandui infection in resistant (IHR 56) and susceptible (MDU1) onion cultivars. Chitinase in both cultivars, with or without challenge-inoculation of A. palandui revealed changes in the isoform pattern. The Native-PAGE of PO showed induction of PO2 isoform in both the cultivars, in response to inoculation of pathogen. Isoform analysis of PPO also exhibited  induction in the Pf1-treated plants challenged with pathogen. Similarly, the activity of b-1,3-glucanase was greatly induced in Pf1-treated plants, challenged with pathogen as compared to controls. Thus, the P. fluorescens-treated plants showed significant increase in the levels of the defense enzymes, in comparison to the plants challenged with the pathogen.

Keywords:     Leaf blight, onion, Alternaria palandui, Pseudomonas fluorescens, induced systemic resistance, defense-related proteins, chitinase, b-1,3 glucanase, peroxidase, polyphenol oxidase

IPC Code:     C12R1/39, C12Q1/28

          E-Mail: karthipath@rediffmail.com

 

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 42, December 2005, pp: 378-385

 

Energy barriers and rates of tautomeric transitions in DNA bases:
Ab initio quantum chemical study

 

Soumalee Basu1$, Rabi Majumdar1, Gourab K Das2 and Dhananjay Bhattacharyya3*

 

Tautomeric transitions of DNA bases are proton transfer reactions, which are important in biology. These reactions are involved in spontaneous point mutations of the genetic material. In the present study, intrinsic reaction coordinates (IRC) analyses through ab initio quantum chemical calculations have been carried out for the individual DNA bases A, T, G, C and also A:T and G:C base pairs to estimate the kinetic and thermodynamic barriers using MP2/6-31G** method for tautomeric transitions. Relatively higher values of kinetic barriers (about 50-60 kcal/mol) have been observed for the single bases, indicating that tautomeric alterations of isolated single bases are quite unlikely. On the other hand, relatively lower values of the kinetic barriers (about 20-25 kcal/mol) for the DNA base pairs A:T and G:C clearly suggest that the tautomeric shifts are much more favorable in DNA base pairs than in isolated single bases. The unusual base pairing A′:C, T′:G, C′:A or G′:T in the daughter DNA molecule, resulting from a parent DNA molecule with tautomeric shifts, is found to be stable enough to result in a mutation. The transition rate constants for the single DNA bases in addition to the base pairs are also calculated by computing the free energy differences between the transition states and the reactants.

Keywords: Tautomerism, DNA, spontaneous mutation, quantum chemistry, transition rate, unusual base pairing, intrinsic reaction coordinate analysis

IPC Code: C07H21/04

          E-Mail:  dhananjay.bhattacharyya@saha.ac.in

 

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 42, December 2005, pp. 386-390

 

 

Reaction of substituted pyrimidines with photochemically generated
t-BuO• radicals

L Charitha and M Adinarayana*

Humans are exposed to various organic peroxides through chemical, pharmaceutical and cosmetic products. On photolysis, these peroxides produce alkoxyl radicals and hydroxyl radicals. The reaction of ·OH radicals with DNA and its constituents have been extensively studied, but very little is known about the reactions of alkoxyl radicals with DNA and its constituents. In view of this, the oxidation of pyrimidine bases viz., thymine, uracil, cytosine, 5-bromouracil, 6-methyluracil and 1, 3-dimethyluracil by t-BuO· radicals in aqueous solution at pH 7.5 has been carried out. The reaction between pyrimidine and t-BuO· is followed by measuring the absorbance of pyrimidine at the respective lmax. The rates of oxidation of pyrimidines are calculated from the plot of absorbance vs time. The rates of oxidation of pyrimidines have been found to increase with increase in [t-BuOOH], [pyrimidine] and light intensity. The quantum yields are calculated from the initial rates of oxidation of pyrimidine and the measured light intensity at 254 nm the wavelength at which t-BuOOH is activated to give radicals. The quantum yields are found to depend on [pyrimidine] as well as on [t-BuOOH] while they are independent of light intensity. The product analysis was carried out on HPLC with UV-visible detector. The corresponding 5,6-dihydroxypyrimidine and isobarbituric acid have been identified by comparing the retention times of the authentic samples. On the basis of experimental results and product analysis, it is suggested that t-BuOOH on photolysis gives t-BuO· radical, which initiates the reaction by adding to C (5) or C (6) position of pyrimidine base, leading to the formation of pyrimidine base radical via hydrolysis. The pyrimidine radical further reacts with t-BuO· radical to give the final product. This study predicts the probable transient pyrimidine radicals.

Keywords: tert-butyl hydroperoxide, t-BuO· radical, pyrimidine bases, oxidation of pyrimidines by t-BuO

·

IPC Code: C07D 239/00, C07H 1906

         E-Mail:  mundra_adinarayana@hotmail.com

 

 

 

Notes

Indian Journal of Biochemistry & Biophysics

Vol. 42, December 2005, pp. 391-394

 

Determination of serum glucose using co-immobilized glucose oxidase and peroxidase onto arylamine glass beads affixed on a plastic strip

Neelima Tank, Suman and C S Pundir*

 

Glucose oxidase (GOD) from Aspergillus niger and horseradish peroxidase (POD) were co-immobilized onto arylamine glass beads affixed on a plastic strip with a conjugation yield of 28.2 mg/g and 43% retention of their initial specific activity. The co-immobilized enzymes showed maximum activity at pH 7.5 when incubated at 37°C for 15 min. A simple, specific and sensitive method for discrete analysis of the serum glucose was developed employing this strip. The minimum detection limit of the method was 5 mg/dl. Within and between assay coefficient of variations for the serum were <5.6% and <10.6% (n = 6) respondely. A good correlation (r = 0.943) was found between the glucose values obtained by the enzyme colorimetric method employing free GOD and POD and the present method. The strip lost 50% of its initial activity after its 150 regular uses for a period of one month, when stored in reaction buffer at 4°C. The method is cost-effective than the enzymic colorimetric method, as the enzyme strip is reusable

          Keywords: Glucose determination, glucose oxidase, peroxidase, arylamine glass, co-immobilization, serum, plastic strip.

IPC Code: C12N 11/00

          E-Mail: pundircs@rediffmail.com

 

 

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 42, December 2005, pp. 395-397

 

Antibacterial activity of crude methanolic extract and its fractions of aerial parts of Anthemis tinctoria

Cahit Akgul* and Gulsen Saglikoglu

 

The antibacterial activity of the methanolic extract and its fractions of aerial parts of Anthemis tinctoria (Asteraceae) was investigated against representative gram-positive Staphylococcus aureus (ATCC 25923) and Enterococcus faecalis (ATCC 29212) and gram-negative strains Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853). The activity was concentrated mainly in the dichloromethane (DCM) and hexane fractions of crude methanolic extract. The 5 mg of DCM extract per disk produced 15-16 mm of inhibition zone against S. aureus and P. aeruginosa, however, no activity was found against E. faecalis and E. coli. The hexane fraction showed activity against S. aureus, P. aeruginosa and E. faecalis. As DCM fraction showed the highest antibacterial activity in the disk diffusion assay, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of only this fraction was determined against S. aureus and P. aeruginosa. These values were found to be in the range of 1.25 to 10 mg/ml.

          Keywords: Anthemis tinctoria, antibacterial activity, Pseudo-monas aeruginosa, Staphylococcus aureus, MIC, MBC

IPC Code: A61P31/04

          E-Mail: cahitakgul@comu.edu.tr; cahitakgul@yahoo.com

 

 

 

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 42, December 2005, pp. 398-400

 

Antibacterial activity of some unsymmetrical diorganyltellurium(IV) dichlorides

Daya Soni, Prabhat K Gupta*, Yatindra Kumar# andT G Chandrashekhar#

 

Six unsymmetrical diorganyltellurium(IV) dichlorides RR'TeCl2 (where R= phenacyl-, 1-naphthacyl-, and styrylacyl- and R' = p-methoxyphenyl, p-hydroxyphenyl-, and 3-methyl-4-hydoxyphenyl-) were tested for their antibacterial activity against gram-positive (Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 25923) and gram-negative (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Salmonella sp.) bacteria. Antibacterial activity was measured by disk diffusion method. Inhibition zones demonstrated that all the compounds showed good activity against gram-negative strains. Phenacyl (3-methyl-4-hydroxyphenyl) tellurium(IV) dichloride and naphthacyl (3-methyl-4-hydroxyphenyl) tellurium(IV) dichloride showed significant activity against both gram-positive and gram-negative strains. Among the tested compounds, the former exhibited maximum activity against gram-positive bacteria, while the latter against all the bacteria under study and styrylacyl (p-methoxyphenyl) tellurium(IV) dichloride against all the three gram-negative bacteria.

        Keywords: Unsymmetrical diorganyltellurium(IV) dichlorides, antibacterial activity, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella sp.

IPC Code: C12Q1/18

         E-Mail:  prabhat@mail.nplindia.ernet.in; dsoni@mail.nplindia.ernet.in

 

 

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 42, December 2005, pp. 401-403

 

Lead levels in some biological samples of auto-mechanics in Abeokuta, Nigeria

O O Babalola*, L O Ojo and M O Aderemi

 

Lead levels were determined in the blood, scalp hair and fingernails of 38, all male auto-mechanics (aged 18-45 years) from Abeokuta, South-western Nigeria. The subjects were classified into four sub-groups based on the period of exposure namely: 1-5, 6-10, 11–15, and >16 years. Thirty-two occupationally unexposed subjects (mainly office workers) served as the control. The weight, height and body mass indexes of all subjects were noted, in addition to other information obtained through structured questionnaire. The mean values of blood lead (BPb), hair lead (HPb) and fingernail lead (NPb) of the occupationally exposed subjects (n=38) were 48.50 ± 9.08 mg/dL, 17.75 ± 5.16 mg/g, and 5.92 ± 3.30 mg/g respectively, while the corresponding mean values for these parameters in the control subjects (n = 32) were 33.65 ± 10.09 mg/dL, 14.30 ± 5.90 mg/g and 5.31 ± 2.77 mg/g respectively. The differences in BPb and HPb levels of the two groups were statistically significant (P <0.05 and P <0.01 respectively), while that of NPb was not significant. The levels of lead in the biological samples appeared to have no relationship with the number of years on the job. From these results, it was obvious that the higher levels of lead in the biological samples of test subjects, compared with those of the controls were from environmental sources.

          Keywords:   Lead, auto-mechanics, occupationally exposed subject, blood, fingernail, hair

IPC Code:      G01N33/00

          E-Mail: segunbablo@yahoo.ca

 

 

      

 


Annual Author Index

 

Abraham P

59

Aderemi M O

401

Adinarayana M

386

Aggarwal L M

127

Aggarwal S

113

Ahlawat S P S

173

Akgul C

395

Ali M A

358

Alice D

371

Anand A

122

Aslan S

190

Babalola O O

401

Balasaraswathi R

228

Basu S

378

Behere D V

7

Behl R

173

Bhadauria M

321

Bhaskaran R

371

Bhatia S

152

Bhattacharyya D

378

Bhide S V

156

Biswas S

145

Cao C

358

Chakrabarti J

238

Chakrabartty P K

287

Chakrabarty R

243

Chakraborti S

19

Chakraborti T

19

Chakraborty S

106

Chandrashekhar T G

398

Charitha L

386

Chatterjee A

205

Chen J

100

Cheng X

166

Chimni S S

233

Cho Young-Su

339

Choi Yong-Lark

339

Choudhary N L

366

Choudhury R

19

Choy H

358

Dahot M U

326

Darbari R

127

Das G K

378

Das K P

287

Das S

238

De M

287

Demir N

182

Demir Y

182

Dhingra J B

173

Dubey R K

301

Dubey R

73

Forouzandeh M

113

Gagandeep

216

Gao Z Q

41

Ge H

222

Ge S

166

Geraldine P

258

Ghasemi S

326

Ghosh B

19

Ghosh Z

238

Gφnenη A

190

Gopal G

81, 271

Goyary D

345

Gupta A K

161, 315

Gupta Anil K

295

Gupta P K

398

Gupta R

233

Hutchinson T E

92

Indra C

13

Irshad M

73

Jabeen T

279

Jadon A

321

Jain P

315

Jang Moon-Sun

339

Jawed A

233

Jayakumar V

371

Jethi R K

113

Josephrajkumar A

243

Joshi D K

122

Kale R K

216

Kamal J K A

7

Kamboj S S

34

Kanwar S S

233

Karthikeyan M

371

Kashkedikar S G

315

Katyare S S

48

Kaur A

34

Kaur H

295

Kaur M

34

Kaur N

161, 295

Kaur P

161, 279

Kaushal R K

233

Kestwal R M

156

Kim Cherol-Ho

339

Kim Jae-Chul

358

Kiran R

113

Kong X

222

Kumar Y

398

Lee Young-Choon

339

Lee Young-Mi

339

Li M

222

Liu J

308

Liu Q

100

Liu W

100

Liu X

100

Liu Z

222

Lodha M L

152

Majumdar R

378

Mallick B

238

Mandal A

19

Manivasagam T

87

Mehta A

54, 254

Mitra C K

141

Moulik S P

205

Mukhopadhyay P

358

Nagarajan S

122

Nandi A

358

Ojo L O

401

Pandya J D

48

Pathak P C

122

Patil N D

250

Pavan Y S

141

Pereira Ben M J

92

Prakash P

118

Prasad S K

173

Pundir C S

186, 391

Qin S

308

Radhika K

371

Rai D V

127

Rajalekshmy R

271

Rajesh M

13

Rajkumar T

81, 271

Ramakrishnan S 

13

Rao A R

216

Rastogi A

92

Reshamwala S M S

250

Roy K

106

Saboury A A

326

Saglikoglu G

395

Saha D

358

Sairam R K

366

Saxena A K

34

Selvaluxmi G

271

Selvaraju K

228

Sengupta C

106

Shanju S

258

Shanmugasundaram P

228

Sharma A

145

Sharma Anjana

178

Sharma R

345

Sharma S

279

Shukla S

321

Shyamala Devi C S

246

Singh J

34

Singh Jagmohan

34

Singh R K

279

Singh T P

279

Singla S K

113

Sinha S

228

Soni D

398

Soni L K

315

Sood S K

34

Srinivasan A

279

Srinivasan V

13

Sritharan M

28

Srivastava N

54, 254

Subramanian P

87

Suchalatha S

246

Sulochana K N

13

Suman

186, 391

Sundaram C A S S

28

Suthakar G

87

 

 

Tandon C

113

Tank N

391

Thomas G

243

Tiwari R

178

Tokgφz D

190

Torun M

190

Tripathi D N

301

Tripathi O

118

Tseng C K

41

Tyagi A

366

Velazhahan R

371

Verma A P S

122

Verma N K

173

Verma R S

54, 254

Wang G C

41

Xu X

100

Xu Zi-Rong

350

Xue Z

166

Yan Xiang-Hua

350

Yang L

166

Yeruva V C

28

Yildirim S

182

Zheng W

222

Zheng X

166

 

Annual Subject Index

 


A

abdominal gases, cardamom in reduction of 245

abiotic stress   295

abscisic acid (ABA)        295
inhibitory effect of               297

N-Acetylchitooligosaccharides, enzymatic
   hydrolysis of                  341

acetylcholine esterase      145
chlorpyrifos-induced alterations in rat brain       54–57
inhibition of 254

acromegaly      76

actin dynamics in monocytes, changes in     13

action potential 118

Acutolysin A, from Agkistrodon acutus, effects
   of metal ions and EDTA on fluorescence and
   caseinolytic activity of    100

adenocarcinoma           271, 324

Adenosine triphosphatase (ATPase)
activity, assay of                 251
activity in pulmonary artery smooth muscle
   microsomes, stimulation by t-butylhydroperoxide                19
chlorpyrifos-induced alterations in rat brain       54–57
cleaving and release by PLC-Bc                92

Aeromonas spp., chitinases from                  343

affinity chromatography, concanavalin A
   CL seralose                  156

Age-related changes in elemental constituents
   and molecular behaviour of bone   
127–129

Aging               77

Agkistrodon acutus, acutolysin A from    100–104

agonists, in inducing hydrolysis of
   phosphatidylinositol-4,5-diphosphate 173

AIDS patients
fatal opportunistic pathogens in 315
immunocomprised                320

alanine, in modulating IRTK and PI3K activities
   and on changes in actin dynamics in monocytes
   (MC), exposed to high glucose concentration 13–18

Alfa feto protein (AFP)           73

alga, Porphyra yezoensis, improved method for
   isolation of photosystem II from             41

alkaline phosphatase
assay of       94
from Elephas trogontherii (Steppe elephant)
   bone, characterization and purification of       
182–185

alkoxyl radicals 386

alkylamine glass, immobilization of enzyme on to 187

allele-specific oligonucleotide (ASO) 308, 313

Allium cepa var aggregatum, induction of resistance
   against Alternaria palandui infection 371–377

Alternaria palandui infection in onion,
   induction of resistance against           371–377

Alzheimer’s disease                    73

amino acids
antiglycating effect of       13
differential regulation of IRTK and PI3K
   activities in human monocytes 13–18
modulation of enzyme activities in insulin-
   mediated signal transduction pathway  13–18

7-aminoactinomycine-D            358

4-aminoantipyrine                  186

β-Aminobutyric acid (BABA)-mediated enhance-
   ment of resistance in tobacco against TMV
   and capability in wounded tobacco plants           166–171

cAMP, membrane permeable analog of        118–121

 

amplified ribosomal DNA restriction
   analysis (ARDRA)    178

Amplimers   84–85

Amplitron       310

Amylase, from Serratia spp.           178–179

amylase activity in cowpea cotyledons  295
embryo is not required for initiation of  161–165

a-Amylase 161, 295
from Bacillus amyloliquefaciens NCIM 2829,
   purification and characterization of       287–293
extraction, determination and purification of       162, 164
gene expression                 163
magnesium ion binding to, thermodynamic
   study of   326–329

amyloglucosidase                  296

amyloid precursor protein (APP) 73
gene             76

amyloidosis     78
cutaneous      77

anaphase promoting complex (APC)                 271
members of family         276

Anaphylaxis, acute                  350

angoumois grain moth, see Silotraga cereallela

Aniline, for degradation of RNA           153

Anthemis cotula, flowers of, antimicrobial
   activity of 395

Anthemis tinctoria, aerial parts of, antibacterial
   activity of 395–397

Antibacterial activity
methanolic extract and its fractions of aerial
   parts of Anthemis tinctoria     395–397
of some unsymmetrical diorganyltellurium
   (IV) dichlorides  398–400

Antibody library construction 355

antibody-ribosome-mRNA (ARM) complexes   350

antiinflammatory effect, Terminalia chebula fruit 246

antimalarial drug                  145

Antioxidant activity of ethanolic extract of
   Terminalia chebula fruit against iso-
   proterenol-induced oxidative stress in rats 246–248

antioxidant enzymes, specific activities of               216

Antioxidants      19

antiproliferative effect, of Arisaema intermedium
   tubers        34

antitumor activity                  364

Antiviral proteins (AVPs)
from Bougainvillea xbuttiana cv Mahara leaves 
   DNase activity of       152–153
   purification of                 152
MAP   152, 154–155
PAP   152, 155
RNase activity of                 153
on viral RNAs           153, 155

apatite            113

Apis mellifera, propolis from 321

Apolipoproteins
and medical conditions
   acquired disorders 75–78
   inherited disorders      75
physiological changes in levels                   75
role in different clinical conditions 73–78
structural characteristics
   Apo AI, AII, AIV        73, 74
   Apo(a)      75
   Apo B-100 and Apo B-48     74
   Apo C-I, C-II, C-III            74
   Apo E       74
   Apo H 74–75
   Apo J         75

Apolipoproteins and acquired disorders
cancer      75
cardiovascular diseases 75–76
central nervous system (CNS)               75
endocrinological disorder          76–77

Apoptosis 193, 276, 358, 360
induction in PC12 cells exposed to ESA          222–226
measurement of           360–361

aprotinin        243

Arabidopsis spp.
protection against                 171
sugar signaling mutants in   299

Arabidopsis thaliana
osmotic stress                 369
P5CS
of      369

arachidonic acid                  222

Archaeal tRNA genes          238

arginine, in modulating IRTK and PI3K activities
   and on changes in actin dynamics in monocytes
   (MC), exposed to high glucose concentration 13–18

Arisaema intermedium, N-acetyl-D-lactosamine
   specific lectins from tubers of               34–40

Arisaema wallichianum, N-acetyl-D-lactosamine
   specific lectins from tubers of               34–40

ascorbic acid, inhibition of iron-induced lipid
   peroxidation                  247

Asialofetuin       34

aspartate aminotransferase                  345

Aspergillus fumigatus, chitinases from                  343

atherosclerosis 190
risk factor for 77

ATPase, see Adenosine triphosphatase

Austin model-1 316

autoantibodies  77

Autoimmune diseases   77–78

auto-mechanics, lead levels in biological
   samples from           401–403

autophagy by lysosomes   324

Autophosphorylation                  358                  361

AUTOREG, for calculation of E-state index   106

Auxins, in promotion of gibberellin biosynthesis 161

avidin-biotin peroxidase complex      273

 

B

BABA, see β-aminobutyric acid

Bacillus amyloliquefaciens-amylase (BAA)                  326

Bacillus amyloliquefaciens NCIM 2829, a-amylase
   from, purification and characterization of        287–293

Bacillus cereus, PLC-Bc from 92

Bacillus circulans, chitinases of 341

Bacillus coagulans, lipase from  233, 235

Bacillus spp., from effluents of gelatin factory                  179

Bacillus subtilis
strains YY 88 and NRRL B 3411, molecular
   mass of
a-amylase from 292
unsymmetrical diorganyltellurium(IV)
   dichlorides against        398

Bacillus thuringiensis, PLC-Bt from 92

Bacteria
chitinolytic  339
oil-degrading property of  180

bacterial strains, methods for identification of                  178

‘ball and stick’ models, heme binding location
   in human MHA              8

barley, see Hordeum vulgare

base pairing, unusual       378

Base pairs, non-Watson-Crick, stability of   384–385

basis set superposition error (BSSE) 378
correction by Morokuma method       380

Bcl2, indicator of poor prognosis in cervical cancer                  271

Becton Dickinson cell quest FACStation 273

bee glue, see Propolis

benzamidazole, antibacterial activity of    398

benzene, 1-chloro-2,4-dinitro-, (CDNB)      216

benzidine, tetramethyl    28

Benzo(a)pyrene 216

benzoic acid, 5,5'-dithiobis-2-nitro-, (DTNB)      216

benzothiadiazole-7-carbothioic acid S-methyl
   ester (BTH)                  166

benzoyl-arg-p-nitroanilide  243

Bigdye terminator                    83

Biocontrol agent, Pseudomonas fluorescens
   strain Pf1 372

biocontrol and biodegrading agents, Serratia spp.            178

Biopolymers, see Surfactant-biopolymer interaction

biosensors, for determination of lactate, in
   muscles and liver            186

Blast, in rice, Pseudomonas fluorescens
   strain Pf1 against        372

BLASTn search 83–84, 272

Blastocysts      173

Bone
age-related changes in elemental constituents
   and molecular behaviour   
127–129
Steppe elephant, characterization and
   purification of alkaline phosphatase from   
182–185

bone marrow, protection of 315

Bougainvillea xbuttiana, AVPs from leaves of,
   RNase and DNase activities of               152

Box-counting principle      142

brachytherapy 272

brain injury      76

breast cancer   76
   oxidative stress in relation to lipid profiles
   in different stages of 190–193

8-Bromo-cAMP, membrane permeable analog
   of cAMP  118
8-Bromo-cGMP, membrane permeable analog
   of cGMP  118

N-bromosuccinimide                 156

5-bromouracil (5BrU)        388

brown planthopper (BPH), see Nilaparvata lugens

BSSE, see Basis set superposition error

Bulge-Helix-Bulge (BHB)        239

Bulked segregant analysis       231

butylatedhydroxytoluene (BHT) 190                  191

t-BuO• radicals, photochemically generated,
   reaction of substituted pyrimidines with           386–389

t-butylhydroperoxide, stimulation of MMP-2
   and Ca2+-ATPase activity in bovine pulmonary
   artery smooth muscle microsomes by              
19–26

 

C

C33A, cervical cancer cell line                  271

c-erbB2, indicator of poor prognosis in
   cervical cancer                  271

c-myc over-expression, indicator of poor
   prognosis in cervical cancer                  271

Ca2+ channel activity in embryonic myocardium 118–121

Ca2+ uptake in microsomes of bovine pulmonary
   artery smooth muscle
, role of MMP-2 in
   oxidant-mediated regulation of  19–26

Ca2+-ATPase activity in bovine pulmonary artery
   smooth muscle microsomes, stimulation by
   t-butylhydroperoxide treatment  19–26

calcium binding loop     283–284
calcium oxalate monohydrate (COM)
   crystal growth                  113
calculi            113

callose deposition                  166

calphostin-C, PKC inhibitor      150

cAMP, see AMP

camphorin, convertion of supercoiled plasmid
   DNA into linear form  155

Canavalia gladiata (Jack bean), concanavalin A
   from seeds of                  157

cancer             75
breast           76
cervix           81
prostate        76
radiation and chemotherapy of, implications in                216

canonical introns                  238

Capsicum annum, pythium disease of,
  
Pseudomonas fluorescens strain Pf1 against  372

carboxymethyl cellulose      343

carcinogen metabolizing enzymes      216

carcinoma, see specific types

Cardamom, see Elettaria cardamomum

cardiomyocytes 247

Cardiomyopathy                  345

cardioprotective effect of Terminalia chebula
   fruit extract 246

cardiovascular diseases  75–76
prevention by Terminalia chebula fruit extract         248

carob, see Ceratonia siliqua

Carotenoids, in formation of vitellin in
   freshwater prawns        258

Carr-Purcell-Meiboom-Gill (CPMG) method,
   to measure T2 of water protons                  123

caseinolytic activity of acutolysin A from
   Agkistrodon acutus
         101

catalase-peroxidases in mycobacteria, iron
   concentration on expression and activity of               28–32

catechins        248

catechol         248

CD, see Circular dichroism

CDC27
homologue in Schizosaccharomyces pombe 274
protein, involvement in radiation response in
   squamous cell cervix carcinoma           271–277

cDNA, see DNA

cell cycle
analysis 358, 360, 362–363
arrest          276

Cell irradiation  81–82, 273

cell lines
A431, H157 and H460         358
cervical cancer, SiHa and C33A                 271
cervical carcinoma derived, expression of
   mrps28 variant in response to radiation 81–86
human cancer 34

cell membrane thermostability, wheat leaves           122–125

central nervous system (CNS)                75

Ceratonia siliqua seedlings, water-deficit stress in
   cotyledons of                  298

cerebral malaria                  145

cerebro spinal fluid (CSF)          73
Apo E concentration in                    76

ceruloplasmin activity, inhibition of iron induced
   lipid peroxidation 247

cervical cancer, indicators of poor prognosis in                  271

cervical carcinoma derived cell line SiHa             81

cervical tumour cells, down-regulation of CDC27 in    277

cervix cancer    81

cetuximab      363

cetyl diethanolyl methyl ammonium bromides
   (CDMAB), interaction with calf thymus DNA   205–214

cetyl ethanolyl dimethyl ammonium bromides
   (CEDAB), interaction with calf thymus DNA   205–214

cetyl pyridinium chloride (CPC), interaction
   with calf thymus DNA           205–214

cetyl trimethyl ammonium bromides (CTAB),
   interaction with calf thymus DNA   205–214

cetyl triphenyl phosphonium bromides (CTPB),
   interaction with calf thymus DNA   205–214

cGMP, see GMP

chaotropic agent                    287

Chelators        100

chemiluminescence                  358

ChiA
cloning of    340, 341–342
expression in E. coli    340, 342
ORF of      341
recombinant
   characterization of       342–343
   refolding and purification of           340–341

Chinese five-pace snake, Agkistrodon acutus,
   acutolysin A from    100–104

Chitin      339, 371

chitinase (s)   371
assay of 373, 375
bacterial 339, 342
from Serratia spp.     178, 179

Chitinase-A (ChiA)-coding gene of Pseudomonas
   sp. BK1, from Porphyra dentate, over-
   expression and characterization of        339–343

chitinolysis     179

Chitinolytic bacteria                  339

chlorocholine chloride (CCC)                  161

p-chloromercuribenzoate (PCMB), inhibitor of
   phospholipase-C                  92

chlorophyll       41

chlorophyll a binding protein                  295

chloroquine    145

Chlorpyrifos
biotransformation into TCP, role of phenobarbitone
                 254
induced alterations in acetylcholinesterase, lipid peroxidation and ATPases  54–57

Cholestasis        77

cholesterol     190
erythrocyte membrane   250

chromatid separation process       276

Chromatography
gel-exclusion 35
ion-exchange 35

chrome azurol S 28

chromophore  237

chronotropic effect                 118
of dibutyryl-cAMP         120

1,8-cineole     395

cinnamomin, convertion of supercoiled plasmid
   DNA into linear form  155

CIP-29, AVP from Clerodendrum inerme        154

circadian clock, synchronization of                 87

circadian rhythms                    87

circular dichroism (CD) study
binding of Heme to HSA          7
surfactant-biopolymer interaction
   with calf thymus DNA    205–206, 208

circular dichroism spectroscopy           287–289

citric acid cycle 345

Clerodendrum inerme, CIP-29 from           154

Cnaphalocrocis medinalis resistance in rice,
  molecular and biochemical markers
   associated with           228–232

collagen, organic matrix of     127

colorimetric assay, residual lipase
   activity in, methods for inhibition of 233–237

complementarity determining region (CDR) 350

concanavalin A (conA) 156, 173
from seeds of Jack bean    157

Connolly accessible area (CAA)  317

Connolly molecular area (CMA) 317

Connolly solvent excluded volume (CSEV)       317

Coronary artery disease          75

Corticosteroid therapy         77

Corticosterone, simplified fluorimetric method for
   extraction and estimation of 48–52

cotton, see Gossypium hirstutum

cowpea, see Vigna unguiculata

COX, see Cyclooxygenase

critical aggregation concentration (CAC) 205, 211

critical micellar concentration (CMC) 205, 212

Cryptochrome, blue light photoreceptor 87

cyclin B, degradation of                  276

cyclin degradation, inhibition of 276

cyclooxygenase
COX-2 vs. COX-1 binding of 2-(5-phenyl-
   pyrazol-1-yl)-5-methanesulfonylpyridines,
   selectivity requirements for          106–111
COX-2 inhibitors                  106

cytochrome P450 induction     254

cytosol           145

 

D

decanoic acid  395

demicellization of surfactants   212

denaturing agents                    34

deoxyribonucleotide triphosphate (dNTP) 81, 271
fluorescent-labeled 308, 309

DHFR, see Dihydrofolate reductase

diabetes mellitus 76
factors involved in development of vascular
   complications in                 13
type 2, uncontrolled, biochemical changes
   in erythrocyte membrane  
250–253

diacetylchitobiose                  339

diacylglycerol   92

1,2-diacylglycerol, human        173

Dichloromethane                  395

2,6-dichloroindophenol (DCIP)    41

2,6-dichlorophenol-indophenol (DCPIP)     217

1,5-diphenylcarbohydrazide          41

Dietary restriction and triiodothyronine (T3)
   regulation of malate-aspartate shuttle
   enzymes in liver and kidney of mice 345–349

differential display                   82
for analysis of gene expression                   81
to study expression profiles of tumour
   biopsy samples                  271

differential expression     81

digoxin, scFv antibody specific for,
   production by ribosome display           350–356

dihydrofolate reductase (DHFR) inhibitors      2,4-diaminopyrido[2,3-d]pyrimidines and 2,4-diaminopyrrolo[2,3-d]-pyrimidines, QSAR
   analysis of           315–320

N,N′-Dimethyaniline                  186

3-4,5-Dimethylthiazol-2-yl-2,5-diphenyl-
tetrazoliumbromide
              222
  
assay 223, 273

diorganyltellurium(IV) dichlorides, unsym-
   metrical, antibacterial activity of   398–400

dipole moment
X- and Y-component of                 316
Z-component of   315, 316, 320

dithiobis-2-nitrobenzoic acid (DTNB) 156, 216

dithionitrobenzoic acid            322

dithiothreitol   145, 233

DNA
bases, tautomeric transitions in, energy
   barriers and rates of 378–385
cDNA of PLA2 from Indian Cobra, sequence of              282

cDNAs, differentially expressed   271
damage in tumour cells, radiation-induced                 277
duplex, stability of              305
microarray technology  308
sequencing   83
thermal denaturation/renaturation in,
   using laser light scattering    301–306

DNase and RNase activities of AVPs from
   leaves of Bougainvillea xbuttiana    152

docosahexaenoic acid (DHA) 222

Drosophila melanogaster, vg and cryb mutants,
   temporal expression patterns of timeless in 87–90

Drug (s)
antimalarial 145
detoxification system in liver                 220
NSAIDs      106

drug resistance 145

dynamic light scattering (DLS), study of
   surfactant-biopolymer interaction with
   calf thymus DNA   205–206, 208

dyslipidemia     75

dyslipoproteinemia              75, 77

 

E

E-state index, 1CHIV for calculation of 106

Ectoenzymes    93, 182

EDTA, see Ethylenediamine tetraacetic acid

EGFR, see Epidermal growth factor receptor

EGTA, see ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid

eicosapentaenoic acid (ESA), induction of
   apoptosis in PC12 cells exposed to  222–226

ELECTRO1, for calculation of E-state index   106

Elephas trogontherii (Steppe elephant), alkaline
   phosphatase from bone, characterization
   and purification of          
182–185

Elettaria cardamomum
medicinal use of          243, 244
trypsin-like protease in  243
–245

Embryo, not required for initiation of a-amylase
   activity in germinating cowpea seeds           161–165

Endochitinases 339

endocrinological disorder 76–77

Energy barriers and rates of tautomeric
   transitions in DNA bases 378–385

energy dispersive X-ray (ED X-ray)            127

enterobacterial repetitive intergenic
   consensus sequence (ERIC-PCR)          178

Enterococcus faecalis, methanolic extract aerial
   parts of Anthemis tinctoria against                  395

enzyme production, extracellular, by strains
   of Serratia spp. from Narmada     178–181

enzymes of starch degradation, effect of
   exogenous sucrose on, and sucrose meta-
   bolism in cowpea seedlings           295–299

enzymic colorimetric method, for deter-
   mination of lactate, in muscles and liver                  186

enzymic UV, for determination of lactate,
   in muscles and liver            186

Epidermal growth factor (EGF) 358

Epidermal growth factor receptor (EGFR)      358
and
HER1 expression, correlation of          358–365
and HER1-tyrosine kinase inhibitor erlotinib,
   degree of radiosensitizing effect of 358–365

indicator of poor prognosis in cervical cancer                  271

epigallocatechin 248

epinephrine     246

Erbitux           364

Erlotinib         358
sensitivity to 361

Erythrina indica seeds, α-D-mannosidase from,
   purification and partial characterization of
        156–160
erythrocyte membrane, biochemical changes in,
   uncontrolled type 2 diabetes mellitus
250–253

ESA, see eicosapentaenoic acid

ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-
   tetraacetic acid (EGTA) 145, 288

ethylenediamine tetra acetic
   acid (EDTA)     81, 156, 233, 288
on fluorescence and caseinolytic activity
   of acutolysin A from Agkistrodon acutus  100–104

exochelin          28

Exochitinases  339

expression patterns, temporal, of timeless in vg and
   cryb mutants of Drosophila melanogaster 87–90

extracorporeal shock wave lithotripsy    113

 

F

F-actin filaments organization in cells
specific binding to phalloidin-FITC           13
treatment with glucose, influence of lysine            13

Femtosecond dynamics of tautomeric transitions    378

fibrinolysis       75

fibrosis, periportal                  324

fish oil, PUFA in                  225

flagellin genotyping                  178

flatulence, cardamom in medicinal
   preparations for              243

flow cytometric analysis       358

flow cytometric DNA analysis           222, 223

flow injection analysis, for determination
   of lactate, in muscles and liver                  186

fluorescence measurement           287, 288

fluorescence microscopy, study of
   surfactant-biopolymer interaction
   with calf thymus DNA    205–207, 209

Fluorescence spectroscopy, analysis of
   acutolysin A from Agkistrodon acutus  100–104

fluorescent-activated cell sorting (FACS)           271, 273

fluorescent image analyzer      310

Fluorimetric method, for estimation of
   corticosterone               48–52

fluorophore    308

Forskolin        120

FrameSeal slide chambers    310

Free radicals, implication in pathogenesis
   of breast cancer         190

FT-IR spectroscopy 287, 289

fungal cell, lysis of                  371

 

G

GA3 and kinetin plant growth regulators    295

Gahwa           243

α- and β-galactosidases                  156

gallic acid       248

Gefitinib         364

Gel-exclusion chromatography                    35

Gelatin factory, Serratia spp. from effluents of                  180

gelonin, from Gelonium multiflorum 155

GenAmp           83

GenBank    83–84, 272, 341

Gene expression          271–272
mitochondrial, regulation of 48
mrps28, differential expression of variant of 81–86

Genepix          310

genes, encoding photosynthetic proteins, sink-
   specific enzymes      295

genetic diseases, human        378

genotyping
flagellin      178
high-throughput variation     308
SNP           308

Gentamicin     396, 397

germination
conversion of starch to sucrose in cowpea
   cotyledons during        295
of cowpea seeds                  161

gibberellic acid 161

Gibberellins     161

glucan            343

glucan oligomers                  371

b-1,3-glucanase         166, 371
assay of      167–168, 373–376

glucocorticoid hormone       48

gluconeogenesis                  345

glucosaminidase, N-acetyl-β-d-                  156

Glucose
determination 391
human monocytes exposed to, differential
   regulation of IRTK and PI3K activities  13–18

glucose oxidase, co-immobilized and per-
   oxidase, for determination of serum glucose           391–394

a-glucosidase 295

Glutaraldehyde coupling      186

Glutathione, inhibitor of phospholipase-C                    92

glycan-bridge   98

a-glycerophosphate shuttle pathways     345

Glycerophosphoinositol            173
quantification of                  175

glycine-betaine 366

glycol chitin   339, 343
preparation  373

glycol chitosan 339, 343

glycolysis       345

glycosylphophatidylinositol (GPI) anchors        182
pattern of proteins released from, by PLC-Bc
   and PLC-Bt 92
-proteins, release by PLC          98

glyxol, phenyl 156

cGMP, membrane permeable analog of        118–121

gonadosomatic index           258

Gossypium hirstutum, seedlings of, osmotic
   adjustment during water stress          366

GPI, see Glycosylphosphatidylinositol

guanidine hydrochloride 287

gynaecomastia  76

 

H

H-7, PKC inhibitor                  150

haemocoagulation                    77

haemoglobin, glycated      250

Hafbauer-Buchner (H-B) eyelet  88

Hamiltonians approximations using
   Austin model-1           316, 317

Hammett’s constant      316

hapten inhibition assay       35–36

Hausdroff-Besicovitch (box-counting principle)     142

HeLa cells 86, 276

Helianthus annus, seedlings of, osmotic
   adjustment during water stress          366

Hemagglutination               35–36

hematopoietic cells                  358

Heme, binding to HSA
CD spectra of 10
characterization of                  8
fluorescence spectra of       9
steady-state fluorescence, CD and optical difference spectroscopic studies       7–12

hemodialysis    77

hemolysin, from Serratia spp. 178

hemolysis, prevention of 247

Hemorrhagins  100

hepato-cellular carcinoma (HCC)          73

hepatocytes
cuboidal     322
vacuolization of                  324

Hepatotoxicity 323

HEPES, see N-2-hydroxyethylpiperzine-
   N-2-ethane sulphonic acid

HER1/EGFR
expression, correlation of          358–365
-tyrosine kinase inhibitor erlotinib,
   degree of radiosensitizing effect of 358–365

high density lipoprotein (HDL)   73, 190

High performance liquid chromatography          190, 191
cation-exchange, of Arisaemia lectins           37

high-throughput variation genotyping   308

highest occupied molecular
   orbital energy (HOMO)    317, 320

honeybees, see Apis mellifera; see also Propolis

Hordeum vulgare, induction of a-amylase in
   embryos of 298

hormone imbalance      76

horseradish peroxidase   350

hot pepper, see Capsicum annum        372

HPLC, see High performance liquid chromatography

HSA, see Human serum albumin

Human cervical carcinoma cell line               81

human plasma apolipoproteins, classification
   and properties of                 74

human renal stone matrix in urolithiasis,
   role of protein inhibitor isolated from   
113–117

human serum albumin (HSA)
binding of heme to
     spectroscopic studies      7–12
-heme complex                    11

Humicola lanuginose, lipolase        234

hybridoma technology   350

hydrocarbons, biodegradation of           180, 181

hydrogen donor/acceptor           315, 316

Hydroxyapatite, main constituents of               127

N-2-hydroxyethylpiperzine-N-2-ethanesulphonic
   acid (HEPES)   222
, 273, 296

hyperchromic effect          211

hypercoagulability                  252

hyperglycaemia          252, 253
chronic         13

hyperlipidemia  75

hypertension    75

hypoalphalipoproteinemia           75

hypolipidemic, Terminalia chebula fruit extract         246

Hypoxia, importance of serum lactate determina-
   tion in diagnosis and management of           186, 189

 

I

imidazole, N-acetyl-         156

immobilization, of serum lactate oxidase        186

Indian cobra, see Naja naja sagittifera

Indian coral tree, see Erythrina indica         156

indigestion, cardamom in medicinal
   preparations for              243

Indole, 4,6-diamidino-2-phenyl (DAPI)                  205

infection and stress, protection in plants against                  152

inositol phosphates in bovine lymphocytes,
   phytomitogen-induced changes in levels of 
173–176
Insulin
normalization of lipid composition in
   erythrocytes                 250

resistance      13

insulin receptor tyrosine kinase (IRTK) activity
   in human monocytes exposed to high glucose
   concentration, differential regulation by
   amino acids               13–18

insulin-mediated signaling pathway, amino
   acids in modulation of enzyme activities in            13–18

intermediate density lipoprotein (IDL)                    73

intrinsic reaction coordinate analysis       378

introns in
archaeal tDNAs                 238
archaeal tRNA                 238
   canonical 238
   non-canonical           238, 241

invertase         295

iodometric method, for estimation of t-BuOOH    386

Ion-exchange chromatography                    35

IRTK, see Insulin receptor tyrosine kinase

isobarbituric acid           387, 389

isoleucine tRNA(TAT)-gene in
   Picrophilus torridus
      238

isonicotinic acid, 2,6-dichloro-, 166

isonicotinic acid hydrazide      28

isoprophyl thiogalaoctoside (IPTG)         81

isopropyl-D-thiogalactopyranoside   340, 350

isoproterenol-induced oxidative stress,
    antioxidant activity of fruits
   of Terminalia chebula against           246–248

isothermal titration calorimetry (ITC),
   study of surfactant-biopolymer
   interaction with calf thymus DNA   205, 207, 209–211

isothermal titration calorimetry  326

 

J

Jack bean, concanavalin A from seeds of 157

jasmonic acid 166, 167

Jurkat cells, Fas-induced apoptosis in 276

JURKAT, T cell leukemia line 173

 

K

Kabat families 351

Kanamycin     340

kDec03, splice variant of mrps28         81

a-ketoglutarate 345

Ki67, indicator of poor prognosis in
   cervical cancer                  271

kidney stone   113

kinetin and GA3 plant growth regulators    295

Kozak sequence           351, 353

Krebs cycle    345, 347

 

L

lactate dehydrogenase (LDH)        222
determination of                  223

lactate oxidase, from Pediococcus sp.                 186
immobilized
   assay of   187
   kinetic properties of 187, 188
   reuse of   187, 188

lactic acid in serum, determination of                  186

Lactosamine, N-acetyl-d-       34

laser light scattering, use in study of thermal denaturation/renaturation in DNA           301–306

Lead levels in auto-mechanics of Abeokuta,
   Nigeria     401–403

leaf protein profile                  228

leaf water, wheat leaves
free/bound  122, 124
NMR transverse relaxation time (T2)            122

leaves, wheat, electrolyte leakage from 122

lecithin-cholesterol acyl transferase (LCAT)        73

lecithinase, from Serratia spp. 178

Lectins   156, 173
Arisaema intermedium and A. wallichianum
   tubers, isolation and characterization of            34–40

Leishmania, inhibition by isolates of
  
Serratia marcescens 181

leucovorin     315

lichenan         343

Lignin, biosynthesis of               376

lipase
of Pseudomonas aeruginosa and P. cepacia, residual activities of              233
from
Serratia spp.     178, 179

lipase activity, residual, in colorimetric assay,
   methods for inhibition of 233–237

lipid peroxidation                 321
chlorpyrifos-induced alterations in rat brain       54–57
levels of 246, 250

lipids, decomposing, sewage treatment for              180

lipolase          233

lipolytic enzymes, commercial grade          234

lipopolysaccharide-lipoprotein complex,
   in outer membrane of bacteria       400

lipoprotein lipase (LPL)           73

lipoprotein (a)  73

liposomes      205

Lipozyme       233

liquid scintillation counter       174

lithotripsy
extracorporeal shock wave
transurethral 113

liver cirrhosis   76

Liver diseases   77

liver injury, CCl4-induced, evaluation of
   hepatoprotective potential of propolis
   extract in  321–324

low density lipoprotein (LDL)   73, 190

Lφwdin’s concept of proton tunneling     378

lowest unoccupied molecular orbital (LUMO)     315, 316, 320

luffin, convertion of supercoiled plasmid DNA
   into linear form                  155

lung cancer    358

Lupinus luteus seedlings, amylolytic activity
   in de-embryonated cotyledons of 161

Lycopersicum esculantum, pythium disease of,
  
Pseudomonas fluorescens strain Pf1 against  372

Lymphoblasts, human        277

lymphocytes, bovine, phytomitogen-induced
   changes in levels of inositol phosphates in          
173–176

Lyophilization 234

lysine, in modulating IRTK and PI3K activities
   and on changes in actin dynamics in monocytes
   (MC), exposed to high glucose concentration 13–18

lysosomes      324

lysozyme       340

M

Macrobrachium lamarreii, yolk protein profiles
   during reproductive cycle           258

Macrobrachium malcolmsonii, yolk protein
   profiles during reproductive cycle           258

Macrobrachium rosenbergii, yolk protein
   profiles during reproductive cycle           258

maize, see Zea mays

Malaria, see Plasmodium falciparum

malate dehydrogenase                  345

malate-aspartate shuttle enzymes in liver
   and kidney, dietary restriction and
   T3 regulation of        345–349

malonaldehyde 222

Malondialdehyde (MDA)  54, 190, 191, 216, 322
formation, prevention of 247

Mannitol        295
as osmoticum 296

mannopyranoside, p-nitrophenyl-α-D-               156

α-D-mannosidase, from Erythrina indica seeds,
   purification and partial characterization
           156–160

mannosidosis  156

Markers
molecular and biochemical, associated with
   Cnaphalocrocis medinalis resistance in rice
          228–232
predictive    271

Markov model 141

Matricaria recutita, esters and lactones from,
   activity against Mycobacterium tuberculosis
   and M. avium                  395

matrix
organic changes in        127–129
transition     142

matrix metalloproteinases                 100

matrix metalloproteinase-2 (MMP-2)
in microsomes, identification of              20–21
indicator of poor prognosis in cervical cancer                 271
in regulation of Ca2+ uptake in pulmonary
   artery smooth muscle microsomes  19–26

maximum diastolic potential (MDP)                  118

membrane integrity, loss of, in wheat leaves           122–125

2-Mercaptoethanol           205, 233

Metal ion, effect on
acutolysin A from Agkistrodon acutus        100
a-amylase activity, from
   Bacillus amyloliquefaciens                287
lectins from tubers of Arisaema intermedium 34

metal-protein interaction   326

metalloproteinase-2, tissue inhibitor of                 19

metastasis, hepatic                    76

Methanopyrus kandleri, Y8 in tRNA gene  239

methemalbumin (MHA)          7
human, heme binding location in, ‘ball and
   stick’ models 8

Methoxypropane, see 1,1,3,3tetramethoxypropane 246

6-methyluracil 388

MHA, see Methemalbumin

micellization of surfactants   212

microalgae, PUFA in                225

Microarray for SNP analysis 308

Microcalorimeter                  327

Micrococcus spp., from effluents of gelatin factory                  180

Microsomes
hepatic       254
preparation of 93
of pulmonary artery smooth muscle
,
   MMP-2 in regulation of Ca2+ uptake  19–26

mineralocorticoid receptors       48

mitochondria, fluorimetric method for
   corticosterone estimation in 48–52

Minisequencing method       313

Mirabilis jalapa antiviral protein (MAP) 152, 154–155

mineralocorticoid receptors       48

mitochondria, fluorimetric method for
   corticosterone estimation in 48–52

mitochondrial ribosomal protein
   small subunit (MRPS)        81

mitogen stimulation                  173

mitogen-activated protein kinase (MAPK)     358

Mitoribosomes  86

mitosis, ubiquitination activity in    276

mitotic index, indicator of poor prognosis in
   cervical cancer                  271

MMP-2, see Matrix metalloproteinase-2

molar refractivity                  317

monoclonal antibodies    350

monocytes, human, exposed to high glucose
   concentration, differential regulation of
   IRTK and PI3K activities in               13–18

3-(N-Morpholinopropane) sulfonic acid (MOPS)  48, 81

Morokuma method, correction of BSSE          380

mrps28
differential expression of variant of in
   response to radiation in cervical carcinoma
   derived cell line              81
splice variant of                    81

MTT, see 3-4,5-dimethylthiazol-2-yl-2,5-diphenyl-
   tetrazoliumbromide

muscuranic effect on heart muscle, by
   Terminalia chebula fruit extract         246

Mutants, vg and cryb of Drosophila melanogaster,
   temporal expression patterns of timeless in 87–90

Mycobacterium avium
DHFR, antifolate activity of   318
esters and lactones from
   Matricaria recutita against                 395
fatal opportunistic pathogens in AIDS          315

Mycobacterium tuberculosis, esters and
   lactones from Matricaria recutita against                  395

myocardial infarction, pathogenesis of                  246

myocardial marker enzymes, isoproterenol on                  246

Myocardium
embryonic, Ca2+ channel activity in        118–121
protection from injury by Terminalia chebula
   fruit extract
248

mycobacteria, expression and activity of catalase-
   peroxidases in, effect of iron concentration on               28–32

Mycobacterium bovis, expression of
   siderophores in                    29

Mycobacterium fortuitum, expression of
   siderophores in                    29

Mycobacterium kansasii, expression of
   siderophores in                    29

Mycobacterium smegmatis, expression of
   siderophores in                    29

Mycobacterium vaccae, expression of
   siderophores in                    29

mycobactin       28

Myocytes        120

myoinositol, acyl substitution in 98

 

N

Naja naja sagittifera, crystal structure of
   phospholipase A2 from venom of        279–285

nematodes, defense responses in plants against 166

nephrolithotomy, percutaneous 113

nephropathy    77, 345

nephrotic syndrome      77

Nicotiana tabacum cv Yunyan 85                  167

Nilaparvata lugens, resistance in rice                  232

p-Nitrophenyl phosphate, in measurement of
   activity of alkaline phosphatase  93

NMR (proton) transverse relaxation time and
   membrane stability in wheat leaves
   exposed to high temperature shock   122–125

Nonanal, 4-hydroxy-2-    54

non-immune libraries       350

Non-selective non-steroidal anti-inflammatory
   drugs (NSAIDs)    106

Non-Watson-Crick base pairs, stability of   384–385

normo-lipidaemia                  252

NSAIDs, see Non-selective non-steroidal
   antiinflammatory drugs

nuclease, from Serratia spp. 178

 

O

Occupational health hazard, lead levels in
   biological samples from auto-mechanics           401–403

octylglycoside, for solubilization of alkaline
   phospatase from microsomal fraction         98

oil-degrading property of bacteria       180

oligo-3-aminopyridine                  400

Oligonucleotides                  309

oncogenes       81
and oncoproteins, selective inhibition of PKC           150

Onion, see Allium cepa

Oocytes, accumulation of yolk during oogenesis    258

oomycetes, defense responses in plants against 166

open reading frame (ORF)          81
of ChiA       341

optical difference spectroscopy (ODS),
   study of binding of heme to HSA      7–12

organic matrix, role in mineralization           113–117

organotellurium trichloride, antibacterial
   activity of 398

Oryza sativa
Cnaphalocrocis medinalis resistance in,
   molecular and biochemical markers
   associated with          228–232
Co27 and Co32, resistant to angoumois
   grain moth 230
expression of ∆Ή-pyrroline-5-carboxylate
   synthetase gene during drought 366–369
Pseudomonas fluorescens strain Pf1
   against pathogens    372

oscillator          87

osmolytes       366

osmoregulation 296
role of accumulated sucrose in    299

osmotic stress 299, 366
in Arabidopsis thaliana      369

osmoticum     295, 296

osteoporosis   127

ovality           317

Overexpression and characterization of ChiA-
   coding gene of Pseudomonas sp.
   BK1 from Porphyra dentata 339–343

overshoot       118

Oxidants           19

oxidative coupling                  186

Oxidative stress   252, 321, 345
in paracetamol-induced pathogenesis:
   Renal damage              59–61
in relation to lipid profiles in different
   stages of breast cancer 190–193
isoproterenol-induced, antioxidant activity
   of T. chebula fruit extract against 246–248
in tumour-bearing fore-stomach and distant
   normal organs of mice 216–221

oxidoreductase, from Pediococcus sp.                  186

oxons, chlorpyrifos                  254

Oxygen radicals 250

 

P

P5CS of rice, expression of 366

pancreatitis, acute and chronic   77

papillomas      217

paracetamol-induced pathogenesis: renal damage 59–61

PC12 cells, see Pheochromocytoma cells

PCMB, see p-Chloromercuribenzoate

PEG, see polyethylene glycol

Penicillium digitatum, BABA-induced
   resistance against        171

per and tim genes, mRNA and protein levels of                    87

peripheral blood lymphocytes (PBLs)        350

periportal fibrosis                  324

Peronospora tabacina, protection against                  171

peroxidase    371
assay of      373
horseradish 392
immobilization of                  391

pesticides        254

Phenobarbitone, effect on cytochrome P450
   activity and chlorpyrifos and 3,5,6-trichloro-
   pyridinol levels in liver and serum 
254–257

phenylalanine ammonia lyase (PAL)        371
activity 228, 230
   influence of Phytophthora cinnamona
      infection 231
   influence of BABA 166, 168–171

phenylmethyl sulfonyl
   fluoride (PMSF) 93, 145, 156, 233, 243, 273

pheochromocytoma (PC12) cells
   exposed to ESA, induction of apoptosis in           222–226

pheophytin       41

phorbol myristate acetate        145

phosphatidylcholine, preferred substrate for PLC-Bt         92

phosphatidylinositol                 173
preferred substrate for PLC-Bc        92

phosphatidylinositol-3-OH-kinase (PI3K)
   activity in human monocytes exposed to
   high glucose concentration, differential
   regulation by aminoacids 13–18

phosphatidylserine                  145

phosphoenolpyruvate carboxykinase                  347

phospholipase
A2 from crude venom of Indian cobra,
   crystal structure of 279–285
C from Bacillus cereus and B. thuringiensis,
   on phospholipids and membrane bound
   GPI anchors               92–99

phospholipid asymmetry  252

phospholipids 250
inositol containing membrane, role in cell
   signal transduction 173

Phosphorothionates                  254

photochemical reactor, Srinivasan Griffin
   Rayonet type                  386

Photopigments, opsin-based   88

photoreceptor, blue light              87

photoreduction activity          41

photosynthetic proteins, genes encoding     295

photosystem II from Porphyra yezoensis,
   improved method for isolation of 41–46

phytoalexins    371

phytohaemagglutinin (PHA)      173

phytomitogens (lectins)
concanavalinA (conA)
induced changes in levels of inositol
   phosphates in bovine lymphocytes         
173–176
phytohaemagglutinin (PHA)   173

Phytophthora cinnamona infection in rice,
   increased PAL activity due to                  231

PI3K, see Phosphatidyl inositol-3-OH-kinase

PicoGreen staining, solid-phase allele-specific
   primer extension in combination with
   modified primers for SNP genotyping  308–313

g-picoline, antibacterial activity of    398

Picrophilus torridus
identification of the missing tDNAs in    238
tRNomics of, comparative analysis with
   other archaeal thermoacidophiles                 238

a- and b-pinenes                  395

Piritrexim (PTX), inhibitor of DHFR         315

plant growth regulators    295

Plants, natural protection against microbial
   and chewing insects         166

Plasmodium falciparum
culture        146
cytosolic stage-specific protein kinase C-
   like activity in          145–150
inhibition by isolates of Serratia marcescens 181
isolation and cytosol preparation  146–147

PLC-Bc, phospholipase-C from Bacillus cereus
cleaving and release of alkaline phosphatase, 92

PLC-Bt, phospholipase-C from
   Bacillus thuringiensis
92

Pneumocystis carinii, fatal opportunistic
   pathogens in AIDS          315

p-Nitroaniline, determination of
   peptidase activity        244

p-Nitrophenyl palmitate (pNPP)           233–237

PNP, see p-Nitrophenyl phosphate

pokeweed (Phytolacca americana) antiviral
   protein (PAP)           152, 155

polarisable continuum model           378, 380

polydispersity index (PDI)  205, 212

polyethylene glycol (PEG)        295
created stress 299

polymerase chain reaction (PCR)                    81

polymorphic GC rich repetitive sequence
   analysis (PGRS) typing                  178

polymyxin B, inhibitor of mammalian PKC                  149

polyols           366

poly-O-phenylenediamine                 186

polyphenol oxidase (PPO)        371
activity, influence of BABA 166, 168–171
assay of      373

polyunsaturated fatty acids (PUFAs)     222

Polyuronides   397

porcine pancreatic lipase          233

Porphyra dentata, overexpression and characteri-
    zation of ChiA-coding gene of
   Pseudomonas sp. BK1 from           339–343

Porphyra yezoensis
improved method for isolation of photosystem II              41–46
use as food  343

Potato, see Solanum tuberosum

Prawns, freshwater (Macrobrachium spp.), yolk
   protein profiles during reproductive cycle    258–261

presenilins 1 (PSEN1) and 2 (PSEN2)      73
genes            76

principal moment of inertia (X, Y, Z)               317

proliferating cell nuclear antigen (PCNA)      276

proline
biosynthesis in plants         366
content       368
estimation and P5CS assay 367

propolis, from Apis mellifera
hepatoprotective potential of  321–324
other medicinal uses            321

prostaglandin synthesis     106

prostanoids     106

Protease
from
Serratia spp.    178, 179
trypsin-like, in cardamom   243–245

protein (s)
carbonyl       59
defense-related                 371
pathogenesis-related (PR) 166
secondary structure elements, fractal studies 141–144

protein folding, fractal approach to principles of           141–144

protein kinase
calcium-dependent (CDPK)     145
cyclin-dependent (CDK)        145

protein kinase C (PKC)
assay          146
developmental expression of          147–148
inhibitors     149–150
-like activity in human malarial parasite
   Plasmodium falciparum  145–150

protein tyrosine kinase         145

proteinase K     88

proteinuria        77

proton tunneling, Lφwdin’s concept of   378

PSII, see Photosystem II

Pseudomonas aeruginosa
chitinases of 341
lipases of, residual activity of              233
methanolic extract aerial parts of
   Anthemis tinctoria against                395
unsymmetrical diorganyltellurium(IV)
   dichlorides against        398

Pseudomonas cepacia lipase                 234
residual activity of               233

Pseudomonas fluorescens, inhibition of
   Alternaria palandui     371

Pseudomonas sp. BK1, ChiA-coding gene
   of, from Porphyra dentate, overexpression
   and characterization of        339–343

pullulan          343

Pulmonary artery smooth muscle, MMP-2 in
   oxidant-mediated regulation of Ca2+
   uptake in microsomes of
19

Pyricularia oryzae, Pseudomonas fluorescens
   strain Pf1 against        372

Pyridines, 2-(5-phenyl-pyrazol-1-yl)-5-methane
   sulfonyl, requirements for binding with
   COX-1 and COX-2 enzyme subtypes 106–111

pyrimidines
2,4-diaminopyrido[2,3-d] and 2,4-diamino
   pyrrolo[2,3-d]-, QSAR analysis          315–320
substituted, reaction with photochemically
   generated t-BuO• radicals           386–389

Pyrocarbonate, diethyl- (DEPC)                  156

pyrogallol autoxidation, inhibition of 218

∆Ή-pyrroline-5-carboxylate synthetase gene,
   expression in rice during drought 366–369

pyrroline-5-carboxylate synthetase   366

pyruvic acid    217

pythium disease of tomato        372

 

Q

QSAR, see Quantitative structure-activity relationships

quantitative structure-activity relationships
   (QSAR) analysis of 2,4-diaminopyrido[2,3-d]
   pyrimidines and 2,4-diaminopyrrolo[2,3-d]-
   pyrimidines as DHFR inhibitors          315–320
of 2-(5-phenyl-pyrazol-1-yl)-5-methane-
   sulfonyl pyridines 106
of COX-1 binding affinity                  109

Quercitin         248

quinazolines 2,4-diamino, inhibitor of DHFR     315

quinonemine dye           186, 188

 

R

RACE (rapid amplification of cDNA end)   81–83, 85, 273

radiation, differential expression of variant
   of mrps28 in response to, in cervical carcinoma
   derived cell line          81–86

radiation response                    81

radiation therapy                    81

radioresistance 364

Radiosensitization           358, 362

randomization test                  317

randomly amplified polymorphic DNA (RAPD)                  178

RAPD markers, associated for BPH resistance                  232

reactive nitrogen species (RNS)                  190

reactive oxygen species (ROS) 54
generation of 252
oxidative stress induced by  216
prime targets of                  190

Red alga, marine, see Porphyra dentata

relative water content (RWC)                  366

renal calculosis, etiology of   113

Renal disease    77

renal stone matrix in urolithiasis, role of
   biomolecules from    113–117

repulsion energy                  317

residual activities of lipases of
   Pseudomonas aeruginosa  233

Reverse transcriptase polymerase chain
   reaction (RT-PCR)    81, 271, 350
analysis for MRPS28, 82
RNA isolation and               82

rheumatoid arthritis    73, 78

Rhizoctonia solani Pseudomonas fluorescens
   strain Pf1 against        372

Rhizomucor miehei, lipozyme                  234

ribosomal intergenic spacer region
   analysis (RISA)        178

ribosome display                  350

Ribosome inactivating proteins (RIPs)                  152

ribulose bisphosphate carboxylase 295

rice, see Oryza sativa

rice microsatellites                  228

ricin, convert ion of supercoiled plasmid
    DNA into linear form  155

RNase and DNase activities of AVPs from
   leaves of Bougainvillea xbuttiana    152

tRNA(CCA) gene                 238
of Picrophilus torridus, secondary structure of  240

tRNA(TAT) gene                 238
classification of                 239
overlapping with RNA(CCA) gene                 242
of Picrophilus torridus      240

tRNA(UAU) gene, of Picrophilus torridus,
   secondary structure of  240

 

S

saccharifying activity, dinitrosalicylic acid
   method to measure      288

salicylic acid (SA)                  166

Salmonella sp., unsymmetrical diorganyl-
   tellurium(IV) dichlorides against        398

salt stress, inhibition of conversion of starch to
   sucrose and sucrose transport                  295

Saponins         397

Sarocladium oryzae, Pseudomonas fluorescens
   strain Pf1 against        372

Scatchard equation/plot 328

scFv, see Single-chain variable fragment

schizont         145

SCRF             380

seedling growth conversion of starch to
   sucrose in cowpea cotyledons during         295

Serratia ficaria, chitinase producer     181

Serratia spp.
environmental strains from Narmada,
   extracellular enzyme production by           178–181

Serum glucose, determination using
   co-immobilized glucose oxidase and
   peroxidase on to arylamine glass beads  391–394

serum lactate, determination of, with alkyl-
   amine glass bound lactate oxidase
186–189

sewage treatment for decomposing lipids           180

sheath blight, in rice, Pseudomonas fluorescens
   strain Pf1 against        372

sheath rot, in rice, Pseudomonas fluorescens
   strain Pf1 against        372

siderophores    28
from
Serratia spp.            178

Sigmoidal-Boltzmann equation      205

SiHa cells         81

SiHa, cervical cancer cell line                  271

Silotraga cereallela, resistance in rice varities        230

single nucleotide polymorphism (SNP)        378
genotyping, allele-specific primer
   extension on microarray for           308–313

single-chain variable fragment (scFv)
   antibody, human, specific for digoxin
   production by ribosome display           350–356

sink-specific enzymes, genes encoding     295

skin
complaints, cardamom for
                 243
diseases        77

snake venom
acutolysin A from Agkistrodon acutus 100–104
phospholipase A2 from crude venom of
   Indian cobra, crystal structure of        279–285

SNP, see Single nucleotide polymorphism

sodium chloride sodium citrate (SSC)           81

sodium lauryl sarcosine     233

sodium pyruvate                  217

Solanum tuberosum, seedlings of, osmotic
   adjustment during water stress          366

solid-phase allele-specific primer extension
   characteristics on biochip in combination with
   modified primers and PicoGreen staining
   method, analysis of   308–313

sonication       340

spectrophotometry                 288
study of surfactant-biopolymer interaction
   with calf thymus DNA           205–207

Spectroscopy
FT-IR 287, 289
IR              127
ODS, study of binding of heme to HSA     7–12
steady-state fluorescence, study of binding
   of heme to HSA         7–12
temperature-scanning     327
UV-visible   327

Sphingosine, PKC inhibitor      150

squamous cell carcinoma, of head and neck                 358
models        364

squamous cell cervix carcinoma, radiation
   response in, involvement of CDC27 protein           271–277

Srinivasan Griffin Rayonet type
   photochemical reactor        386

Staphylococcus aureus
methanolic extract aerial parts of
   Anthemis tinctoria against                 395
unsymmetrical diorganyltellurium(IV)
   dichlorides against        398

starch degradation and sucrose metabolism
   in cowpea seedlings, effect of exogenous
   sucrose on enzymes of 295–299

starch phosphorylase                  295

Staurosporine, inhibitor of mammalian PKC                  149

Steady-state fluorescence spectroscopy,
   study of binding of heme to HSA      7–12
Steppe elephant, Elephas trogontherii, alkaline
   phosphatase from bone, characterization
   and purification of          
182–185

Stern Volmer plots for iodide and acrylamide   290

Stimulant, cardamom, digestive      243

Stratagene 351, 354

stress (es)
and infection, protection in plants against 152
osmotic      299
oxidative, in paracetamol-induced
   pathogenesis: Renal damage              59–61
PEG-created 299
sucrose and mannitol-induced, reduced
  
b amylases activity       297
temperature 122–125

stretch bend energy           317, 320

struvites         113

subarachnoid haemorrhage 76

Succinic dehydrogenase                  324

sucrose density gradient centrifugation (SDGC)        41

sucrose phosphate synthase (SPS) activity in
   different parts of Cowpea seedlings     295

sucrose synthase                  295

sugar signaling mutants in Arabidospis 299

Sulfolobus solfataricus, tRNomics of, compared
   with Picrophilus torridus      238

Sulfolobus tokodaii, tRNomics of, compared with
   Picrophilus torridus      238

sulphorhodamine B                    34

sunflower, see Helianthus annus

Sunnhemp rosette virus (SRV) 152

supraventricular arrhythmias 350

surfactant-biopolymer interaction with calf
   thymus DNA           205–214

surfactants, demicellization/ micellization of                  212

Systemic acquired resistance (SAR), in tobacco   166

Systemic lupus erythematosus (SLE)           77

systemic resistance, induced       371

 

T

T cell leukemia line, JURKAT                  173

T cells, auto-reactive         77

T-lymphocytes, human, T3 complex on  173

Tangier’s disease 75

tannins          397

tautomeric transitions, Femtosecond dynamics of 378

TEAE-cellulose anion-exchange chromatography           182, 183

Temporal expression patterns of timeless in vg
   and cryb mutants of Drosophila melanogaster 87–90

tensiometry, study of surfactant-biopolymer
   interaction with calf thymus DNA   205–207

Terminalia chebula, fruit extract of
antiinflammatory effect of     246
antioxidant activity, against isoproterenol-
   induced oxidative stress          246–248
cardioprotective effect of     246
cardiovascular diseases, prevention by 248
hemolysis, prevention of 247
hypolipidemic                 246
MDA formation, prevention of 247
muscuranic effect on heart muscle       246
myocardium, protection from injury by    248
thrombogenic effect of      247

1,1,3,3tetramethoxypropane    190, 191, 246

tetramethylthiourea, antibacterial activity of    398

thermal denaturation/renaturation in
   DNA using laser light scattering    301–306

Thermoacidophiles
archaeal, general features of  239
euryarchaeal 238
tRNomics of           240–241

Thermocycler  310

Thermogravimetery                  127

Thermoplasma acidophilum, tRNomics of,
   compared with Picrophilus torridus      238

Thermoplasma volcanium, tRNomics of,
   compared with Picrophilus torridus      238

thiobarbituric acid (TBA)         217

thiobarbituric acid reactive substances
   (TBARS)  190, 191, 250, 251

thiobarbituric acid                  322

thrombogenic effect of Terminalia chebula
   fruit extract
247

thylakoid membranes, isolation of PSII
   particles from               41–46

thymocytes    176

thyroid dysfunction   76

Thyroid hormone           345, 347

Timeless (tim)
expression patterns of    87
per
genes, mRNA and protein levels of                   87
temporal expression patterns in vg and cryb
   Drosophila melanogaster mutants   87–90

TIMP2, indicator of poor prognosis in
   cervical cancer                  271

TMV, see Tobacco mosaic virus

Tobacco mosaic virus (TMV) 152
BABA-mediated enhancement of resistance
   against     166–171

Tobacco, see Nicotiana tabacum

Tobamoviruses 152

tomato, see Lycopersicum esculantum  372

Toxoplasma gondiii
DHFR, antifolate activity of   318
fatal opportunistic pathogens in AIDS          315

transfection    205

transition matrix                  142

Transplantation, liver              77

transurethral lithotripsy    113

trichloromethylperoxy radicals 323

3,5,6-trichloropyridinol (TCP)    254
extraction and estimation of    255–256

triethylamine, antibacterial activity of    398

Trifolium pratenase                        231

Trigonella foenum-graecum seedlings, water-
   deficit stress on sugar content in                298

triiodothyronine (T3) and dietary restriction
   regulation of malate-aspartate shuttle
   enzymes in liver and kidney of mice 345–349

trimethoprim  315

triphenylarsine oxide, antibacterial activity of    398

Triticum aestivum
cultivars
   HD 2428, heat-susceptible                 122
   NIAW 845, heat-tolerant 122
leaves exposed to high temperature shock,
   proton NMR transverse relaxation time and
   membrane stability in   122–125
seedlings of, osmotic adjustment during
   water stress                  366

triton X-100 (TX-100)           217
for solubilization of alkaline phospatase from
   microsomal fraction         98

tRNomics: comparative analysis of
   Picrophilus torridus with other
   archaeal thermoacidophiles                 238

trophozoite    145

Trypsinization   81, 273

trypsin-like protease in cardamom   243–245

Tryptophan fluorescence emission spectrum     288

Tryptophans, quenching of 292

Tumors
high-grade  271
oxidative stress in fore-stomach bearing
   tumour  and distant normal organs of mice          216–221
spontaneous and chemically- induced      345
suppressors   81, 271

tumour cells, radiation-induced DNA damage in                  277

Turner syndrome 77

tyrosine kinase activity        358

 

U

ubiquitination activity in mitosis                  276

ureteroscopy  113

urinary complaints, cardamom for                  243

Urolithiasis, management of           113–117
uromucoid      113

UV spectrophotometry                326

 

V

Vacuolation, perinuclear  322, 323

VALSTAT program      317

Vancomycin   396, 397

very low density lipoprotein (VLDL)        73

Vibrio spp., from effluents of gelatin factory                  180

Vigna unguiculata seedlings
embryo not required for initiation of
  
a-amylase activity in    161–165
exogenous sucrose on enzymes of starch
   degradation and sucrose metabolism in           295–299

viscometry, to study surfactant-biopolymer
   interaction with calf thymus DNA   205–206, 208

vitamin E 19, 190, 247
in reducing oxidative damage                  193

Vitellin            258

vitellogenin     258

 

W

wastes, biodegradation of           180, 181

water deficit, inhibition of conversion of starch
   to sucrose and sucrose transport                  295

water-deficit stress
in cotyledons of carob seedlings                 298
on sugar content in Trigonella foenum-graecum
   seedlings  298

water stress, osmotic adjustment in seedlings of
cotton        366
maize rice   366
potato        366
sunflower   366
wheat          366

wheat, see Triticum aestivum

wound healing  77

Wounding in tobacco, influence of BABA on    166

 

X

Xanthomonas campestris pv oryzae        231

xenogenicity   351

X-ray, energy dispersive (ED X-ray)         127

 

Y

Yolk protein (vitellin) profiles of Macrobrachium
   spp. during reproductive cycle    258–261

Yunyan 85, cultivar of Nicotiana tabacum      167

 

Z

Zea mays, seedlings of, osmotic adjustment during
   water stress                  366

Zebu Sahiwal cow                  174

zeitgeber time   87

zinc metalloenzyme                  182

zinc-metalloproteinases                 100


 

List of Referees

 

Indian Journal of Biochemistry and Biophysics (IJBB) is grateful to the following referees for their honorary contribution in evaluating/reviewing the papers submitted for publication in the journal:

 


Aggarwal P K, Delhi

Aminuddin, Lucknow

Anandakumar P, Delhi

Appukuttan P S, Thiruvananthapuram

Aruldhas M, Chennai

Awasthi R P, Pantnagar

Balasaraswathi R, Coimbatore

Balasingh J, Thirunelveli

Balasubramanian D, Hyderabad

Balasubramanian K A, Vellore

Balasundaram D, Singapore

Bandopadhyay A, Kolkata

Bandyopadhyay D, Delhi

Banerjee U C, Mohali

Bansal D D, Chandigargh

Bansal M, Bangalore

Barma M, Mumbai

Basu S K, Delhi

Behari J R, Lucknow

Ben M J, Roorke

Bhakuni V, Lucknow

Bhan S, Delhi

Bhansali A, Chandigarh

Bharadwaj M, Noida

Bhatt K K, Mysore

Bhatt S G, Mysore

Bhatt S R, Delhi

Bhattacharya S, Kolkata,

Bhattacharyya Dipankar, Kolkata

Bhau B S, Jorhat

Bihari V, Lucknow

Bisaria V S, Delhi

Borah R C, Jorhat

Bowtell D L David, Australia

Brahmachari S K, Delhi

Chakrabarti T, Nagpur

Chakraborty P, Kolkata

Chari K V R, Mumbai

Chandra Naresh, Delhi

Chandrasekaran M K, Bangalore

Chandrashekar A, Mysore

Chatterjee B P, Kolkata

Chatterjee I B, Kolkata

Chatterji D, Bangalore

Chattopadhyay A, Hyderabad

Chattopadhyay Dhrubjyoti, Kolkata

Chattopadhyay S, Mumbai

Cullinane C, Australia

Das H S, Delhi

Das M, Lucknow

Dattagupta J K, Kolkata

Devasagayam T P A, Mumbai

Devotta S, Nagpur

Dhaneshwar S R, Pune

Dikshit Madhu, Lucknow

D’Souza C, Mysore

Dwarakanath B S, Delhi

Ganesan K, Chandigarh

Garg G K, Pantnagar

George A, Delhi

Ghosh A, Chandigarh

Ghosh S, Kolkata

Girdhar R, Baroda

Giridhar P, Mysore

Goltsvev V, Bulgaria

Gopalakrishnakone P, Singapore

Gopalan C, Delhi

Gopinathan K P, Bangalore

Goswami K, Kolkata

Govindarajan R, Lucknow

Govindjee, Illinois

Gowda L, Mysore

Gowrishankar J, Hyderabad

Goyal N, Lucknow

Grover A, Delhi

Grover Anil, Chandigargh

Gunasekaran P, Madurai

Gupta C M, Lucknow

Gupta M N, Delhi

Gupta R L, Delhi

Gupta S K, Delhi

Gupta Satish K, Delhi

Gupta Y K, Lucknow

Hasnain S E, Hyderabad

Hosur R V, Mumbai

Jacob M, Vellore

Jagannathan N R, Delhi

Jain R K, Chandigarh

Jarori G K, Mumbai

Jawali N, Mumbai

Jesudason A, Chennai

Jetty A, Hyderabad

Joshi M, Bangalore

Kale R K, Delhi

Kanaujia V P, Kanpur

Kapoor H C, Delhi

Karanth N G, Mysore

Kasturi S R, Mumbai

Katyare S S, Vadodara

Kaushik, B D, Delhi

Khan M Y, Delhi

Khanduja K L, Chandigarh

Khanna H D, Varanasi

Khanna N, Delhi

Khanuja S P S, Lucknow

Khare S K, Delhi

Khullar G K, Chandigarh

Kishore R, Chandigarh

Krishna Sudhir, Bangalore

Krishnamoorthy G, Mumbai

Krishnamurthy H, Bangalore

Kukreti S, Delhi

Kulkarni V M, Pune

Kumar A, Indore

Kumar A, Pune

Kumar S, Bangalore

Kundu Bijoy, Lucknow

Kundu S C, Kharagpur

Lakhotia S C, Varanasi

Lakshmanan M, Madurai

Lakshmanaperumalsamy P, Coimbatore

Lee L, Maryland, USA

Lee Y C, Busan, Korea

Leem S H, Busan, Korea

Lodha M L, Delhi

Lokesh B R, Mysore

Madan T, Delhi

Mahanta P K, Bangalore

Maheshwari R, Bangalore

Maithal Kapil, Ghaziabad

Maiti S, Delhi

Majumdar G C, Kolkata

Majumdar H P, Kolkata

Maliekal T, Bangalore

Malleshi, Mysore

Manchanda R, Mumbai

Mandal A B, Chennai

Mandal A, Kolkata

Marita A R, Mumbai

Mazumdar S, Mumbai

Mehra R, Ajmer

Mejoo S, Bangkok

Mishra B, Karnal

Misra A K, Lucknow

Misra A N, Bhubaneswar

Mitra C K, Hyderabad

Mitra S, Kolkata

Mohanty P, Bhubaneswar

Moulik S P, Kolkata

Mukherjee S, Delhi

Mukherjee S N, Pune

Mulimani V H, Gulbarga

Muniyappa K, Bangalore

Murthy B, Padappai, Tamil Nadu

Murthy M R N, Bangalore

Murugesan K, Chennai

Musarrat J, Aligarh

Nagappa A N, Pilani

Nagaraj R, Hyderabad

Nath U, Bangalore

Nautiyal S, Lucknow

Oommen Anna, Vellore

Pal Debnath, Bangalore

Palepu R, Canada

Panneerselvam C, Chennai

Pant K K, Delhi

Parthasarathy V A, Calicut

Pasha Santosh, Delhi

Pasupathy K, Mumbai

Pathre U V, Lucknow

Patnaik P R, Chandigarh

Pillai Sreekumar S, Thiruvananthapuram

Ponnusamy M N, Chennai

Prakash V, Mysore

Prasad R, Delhi

Pratap R, Lucknow

Prohit H J, Nagpur

Pugazhendi K V, Annamalinagar

Pundir C S, Rohtak

Punekar N S, Mumbai

Pushpangadan P, Lucknow

Qadri A, Delhi

Raghava G P S, Chandigarh

Raghavan, Mysore

Raghubir R, Lucknow

Rai A N, Itanagar

Rajagopal V, Kasaragod

Rajeshkumar B, Massachusetts

Rajeshwari M R, Delhi

Rajkumar T, Chennai

Ram G C, Izatnagar

Ramaiah K V A, Hyderabad

Ramakrishnan C, Bangalore

Ramakrishnan S, Chennai

Ramanathan K V, Bangalore

Ramasami T, Chennai

Ramasarma T, Bangalore

Ramaswamy R, New Jersy

Ramesh H P, Mysore

Ramesh Chander, Mumbai

Ranjit M R, Bhubaneswar

Rao A, Mysore

Rao A B, Hyderabad

Rao A G Appu, Mysore

Rao B J, Mumbai

Rao C P, Mumbai

Rao Ch Mohan, Hyderabad

Rao D N, Delhi

Rao D Narasimha, Bangalore

Rao K Subba, Hyderabad

Rao, M R S, Bangalore

Rao N M, Hyderabad

Rath B K, Varanasi

Ravindranath T, Delhi

Ravindranath V, Manesar

Ravishanker, Delhi

Rawal S K, Pune

Ray M, Kolkata

Ray S, Kolkata

Reddanna P, Hyderabad

Rodrigues V, Mumbai

Roy A K, Bhagalpur

Roy K, Kolkata

Roy R P, New Delhi

Roy S, Kolkata

Sagi Amir, Israel

Sahani M S, Bikaner

Sainis K B, Mumbai

Sakai H, Japan

Saleemuddin M, Aligarh

Sarkar Debi P, Delhi

Satoh Kimiyuki, Okayama, Japan

Satyanarayana T, Delhi

Savithri H S, Bangalore

Saxena A K, Lucknow

Seetharama N, Hyderabad

Sehgal Devinder, Delhi

Sen P C, Kolkata

Setty O H, Hyderabad

Shamala T R, Mysore

Sharma Pushkar, Delhi

Sharma Shobhona, Mumbai

Shetye S R, Goa

Shivaji S, Hyderabad

Shoji R, Tokyo, Japan

Shukla R, Lucknow

Singh Anil K, Mumbai

Singh B D, Varanasi

Singh B N, Lucknow

Singh H B, Mumbai

Singh M P, Lucknow

Singh P, Delhi

Singh R, Delhi

Singh S A, Mysore

Singh T P, Delhi

Singh Lalji, Hyderabad

Sitaraman V, Pune

Sivakami S, Mumbai

Sodhi Ajit, Varanasi

Somasundaram S G, Texas

Sopory S K, Delhi

Sridevi, Mysore

Srinivas U, Hyderabad

Srinivasan, Mysore

Strasser Reto J, Geneva

Sudhakaran P R, Thirvananthapuram

Sureshkumar G, Kolkata

Suryakar A N, Solapur

Suryaprakash G, Gulbarga

Swaminathan M S, Chennai

Swamy B M, Dharwad

Swarup Ghanshyam, Hyderabad

Talwar K K, Chandigargh

Tharanathan R N, Mysore

Tyagi A K, Delhi

Unni B G, Jorhat

Varadaraj M C, Mysore

Varalakshmi P, Chennai

Varshney U, Bangalore

Vedasironmani J R, Kolkata

Velmurugan D, Chennai

Vishveshwara S, Bangalore

Wahidullah S, Goa

Wang X, Nanjing

Wategaonkar S, Mumbai

Watts M, Tasmania

Yadav J S, Hyderabad

Yadav M P, Izatnagar

Yadav Sushma, Texas

Yathindra N, Bangalore