Indian Journal of Biochemistry & Biophysics

CODEN: IJBBBQ  ISSN: 0301-1208

http://www.niscair.res.in; http://nopr.niscair.res.in

Total visitors: 4,815  since 07-01-09

 

VOLUME 45

NUMBER 6

DECEMBER 2008

 
 
    CONTENTS

Minireview

 

Localization of Heme Biosyntheis Pathway Enzymes in Plasmodium falciparum

365

         Aditya Rao, Sri Jyothsna Yeleswarapu, Rajgopal Srinivasan and Gopalakrishnan Bulusu*

 

 

 

Papers

 

A new method for purification of functional recombinant GST-cyclophilin A protein from E. coli

374

         Seonmin Lee, Xuezhe Han, Kyu Jin Choi, Yan Ding, Taegyu Choi, Eunyoung Tak, Jisun Lee, Joohun Ha, Sung Soo Kim* and Jinhwa Lee

 

 

 

Purification and characterization of sulfite oxidase from goat liver

379

        Ausaf Ahmad*, Sarfraz Ahmad and Masroor A Baig

 

 

 

Cloning and characterization of drought responsive partial gene sequence(s) from
Oryza sativa L. subsp. indica

387

        Satendra K Mangrauthia, Nandlal Choudhary and Aruna Tyagi*

 

 

 

Tri-iodothyronine alters superoxide dismutase expression in a teleost
Anabas testudineus

393

        P Sreejith and O V Oommen*

 

 

 

Effect of Na+ ions on pH-dependent conformational changes in brush border sucrase-isomaltase in mice intestine

399

         Shiffalli Gupta, Safrun Mahmood, Rizwan Hasan Khan and Akhtar Mahmood*

 

 

 

Optimization of extracellular xylanase production by Sclerotinia sclerotiorum S2 using factorial design

404

         Olfa Ellouze*, Sami Fattouch, Faouzi Mestiri, Mohamed Radhouen Aniba and Mohamed Nejib Marzouki

 

 

 

Dietary cholesterol and estrogen administration elevate brain apolipoprotein E in mice by different mechanisms

410

        Neelam Srivastava, Maurizio Averna and Rai Ajit K Srivastava*

 

 

 

Semi-empirical calculations on paullones, a promising class of cyclin-dependent kinase inhibitors

416

        Pooja Sapra Sharma, Reena Tyagi & Rajan Sharma

 

 

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

        G K Solanki, Anand Amin, Ashvin Padhiyar, A K Ray and A T Oza*

 

 

 

 

 

TRendy’s Meeting Report 2008

430

 

 

Annual Author Index

433

 

 

Annual Subject Index

435

 

 

List of Referees

447

 

 

 

—————

*Author for correspondence

 

 

AUTHOR INDEX

 

 

Ahmad A 379
Ahmad S 379
Amin A 421
Aniba M R  404
Averna M  410
   
Baig M A 379
Bulusu G 365
Choi K J  374
Choi T  374
   
Choudhary N  387
Ding Y 374
Ellouze O 404
Fattouch S 404
Gupta S 399
   
Ha J 374
Han X 374
Khan R H 399
Kim S S 374
Lee Jinhwa 374
Lee Jisum 374
   
Lee S 374
Mahmood A 399
Mahmood S  399
Mangrauthia S K 387
Marzouki M N 404
Mestiri F 404
Oommen O V 393
Oza A T 421
Padhiyar A 421
   
Rao A   365
Ray A K 421
   
Sharma P S 416
Sharma R  416
Solanki G K 421
   
Sreejith P 393
Srinivasan R 365
Srivastava N 410
Srivastava Rai A K 410
   
Tak E 374
Tyagi A 387
Tyagi R 416
Yeleswarapu S J 365

 

 

MINIREVIEW

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 45, December 2008, pp. 365-373

 

 

Localization of Heme Biosynthesis Pathway Enzymes in Plasmodium falciparum

Aditya Rao, Sri Jyothsna Yeleswarapu, Rajgopal Srinivasan and Gopalakrishnan Bulusu*

Life Sciences R&D Division, TCS Innovation Labs Hyderabad, Tata Consultancy Services Ltd, 1 Software Units Layout, Madhapur, Hyderabad 500081, India

Received 31 January 2008; revised 01 September 2008

Protein trafficking in the malarial parasite Plasmodium falciparum is dictated by a complex life-cycle that involves a variety of intra-cellular and host cell destinations, such as the mitochondrion, apicoplast, rhoptries and micronemes. Of these, the apicoplast and mitochondrion are believed to account for more than 10% of this traffic. Studies have shown that mechanisms for mitochondrion and apicoplast targeting are distinct, despite their close physical proximity. The heme biosynthesis pathway spans both these organelles, making trafficking studies crucial for the spatial demarcation of the constituent interactions. This minireview highlights the challenges in identifying the possible sub-cellular destinations of the heme pathway enzymes using gleanings from literature survey as well as focussed bioinformatic analysis.

Keywords: Plasmodium falciparum, Localization, Apicoplast, Mitochondrion, Heme biosynthesis, Heme pathway enzymes

*E-mail: gopal@atc.tcs.com

 

 

 

PAPERS

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 45, December 2008, pp. 374-378

 

 

A new method for purification of functional recombinant GST-cyclophilin A protein from E. coli

Seonmin Lee+, Xuezhe Han1+, Kyu Jin Choi, Yan Ding, Taegyu Choi, Eunyoung Tak, Jisun Lee, Joohun Ha, Sung Soo Kim* and Jinhwa Lee2,*

Department of Biochemistry and Molecular Biology, Medical Science and Engineering Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute (BK-21), School of Medicine, Kyung Hee University, Seoul 130-701, Korea

1Department of Neurosurgery, The First Hospital, Jilin University, Changchun, China; 2Department of Biomedical Laboratory Science, Dongseo University, Busan 617-716, Korea

Received 28 April 2008; revised 20 August 2008

The expression of glutathione-S-transferase (GST) fusion protein is extensively utilized in the study of protein-protein interactions. In the commonly used purification method, the overexpressed GST fusion protein is bound to the glutathione (GSH)-coupled resins via affinity chromatography, and then eluted by an excessive quantity of reduced GSH. However, this technique has certain limitations, such as low product purity, retention of GSH in the sample, as well as relatively high cost. To overcome these limitations, in this study, elution buffer containing 2% formic acid was utilized rather than GSH to elute the GST-fusion protein, and thereafter the acidic samples were neutralized using collecting buffer. By using this method, highly purified GST-cyclophilin A (CypA) fusion protein was obtained, without affecting the structural and functional characteristics such as PPIase and chaperone activities. Moreover, the procedure is also cost-effective, due to the low cost of formic acid as compared with GSH.

Keywords: Chaperone activity, Cyclophilin A, Formic acid, Gluthatione-S-transferase fusion protein, PPIase activity

*E-mail: sgskim@khu.ac.kr, jinhwa2000@gdsu.dongseo.ac.kr

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 45, December 2008, pp. 379-386

 

 

Purification and characterization of sulfite oxidase from goat liver

Ausaf Ahmad1*, Sarfraz Ahmad2 and Masroor A Baig1

1Department of Biochemistry, Faculty of Science, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi 110 062, India

2Florida Hospital Medical Center, Cancer Institute, 2501 N. Orange Ave., Suite 883, Orlando, FL 32804, USA

Received 21 February; revised 21 November 2008

Sulfite oxidase (EC 1.8.3.1) catalyzes the physiologically vital oxidation of sulfite to sulfate, the terminal reaction in the degradation of sulfur containing amino acids. Genetic deficiency related to human sulfite oxidase is associated with the severe clinical abnormalities with no effective therapies known, making the enzyme of immense biomedical importance. In the present study, sulfite oxidase was been purified from the goat tissues, a hitherto unexplored source, in particular from the liver, and its physico and biochemical properties were characterized. The liver was chosen as it showed the highest activity, compared to kidney and muscle. The enzyme was purified to homogeneity by salting out, gel filtration and ion-exchange chromatography. It was a dimer (113 kDa) having two identical subunits (56 kDa) and did not contain free sulfhydryl groups. Its spectral analysis showed the presence of heme and molybdenum. circular dichroism (CD) spectra in near and far-UV regions showed the presence of significant amounts of secondary structures (45% a helix, 9% b structure and 26% b turn and remaining random coil) in the native molecule. The kinetic and hydrodynamic properties of the enzyme were also determined. Results also showed that ferricyanide was 8-times more effective electron acceptor than its physiological acceptor cytochrome c. The limited N-terminal analysis of the enzyme revealed the sequence up to six amino acids Trp-Glu-Pro-Ser-Gly-Ala. Together, these results suggested the liver was a major source of sulfite oxidase in goat and most of its physico-chemical, except secondary structure and amino acid sequence from N-terminal and biological properties were fairly similar to the sulfite oxidase isolated from other mammalian species/organs.

Keywords: Goat liver, Sulfite oxidase, Molybdoenzyme, Molybdenum, Heme, Purification, Physico-chemical properties, CD spectra, Absorption spectra, N-terminal analysis

*E-mail: ausaf_ahmad@urmc.rochester.edu

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 45, December 2008, pp. 387-392

 

 

Cloning and characterization of drought responsive partial gene sequence(s) from Oryza sativa L. subsp. Indica

Satendra K Mangrauthia, Nandlal Choudhary and Aruna Tyagi*

Division of Biochemistry, Indian Agricultural Research Institute, Pusa Campus, New Delhi 110012, India

Received 19 April 2008; revised 29 August 2008

Differential display gels were run for the drought tolerant (N-22) and drought susceptible (Panidhan) genotypes of rice (Oryza sativa) to identify the genes showing differential expression with respect to moisture stress. Differential cDNA products were cloned in PCR-Trap vector and analyzed for differential expression by Northern hybridization. Two clones namely R4A and R7G were found to be associated with water deficit stress (WDS). Sequencing revealed an insert of 244 bp in the clone R4A. BLASTN and FASTA results showed that R4A had maximum homology with a full-length cDNA clone: 002-110-H10 and OSJNBa006109.12 protein. GO classification suggested that it had b-glucosidase motif which had been implicated in ABA mobilization and thereby ABA dependent gene expression.

Keywords: Water deficit stress, Differential expression, cDNA, Oryza sativa, Drought responsive gene

*E-mail: at_bio@iari.res.in

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 45, December 2008, pp. 393-398

 

 

Tri-iodothyronine alters superoxide dismutase expression in a teleost
Anabas testudineus

P Sreejith and O V Oommen*

Department of Zoology, University of Kerala, Kariavattom, Thiruvananthapuram-695 581, Kerala, India

Received 24 January 2008 ; revised 11 September 2008

The effect of tri-iodothyronine (T3) on superoxide dismutase (SOD) expression was evaluated in a teleost Anabas testudineus (euthyroid fish) by native gel electrophoresis and Western blot analysis. SOD is an essential enzyme for the survival of oxygen-utilizing organisms. Its expression is altered by the stress, presumably due to the increase in concentration of superoxide radical in cells. Variations of thyroid hormone levels are the major physiological modulators of cellular oxidative stress. T3 administration generates an oxidative stress, which to some extent is neutralized by the changed activity of enzymes like SOD. T3 treatment decreased CuZn SOD density in liver and brain of A. testudineus. The activity of CuZn SOD in liver and brain was confirmed by native gel analysis. The different physiological states of thyroid influenced the CuZn SOD activity. Western blot analysis further confirmed that liver and brain CuZn SOD decreased after T3 treatment. From these findings, it was clear that T3 treatment in euthyroid fish created an oxidative stress condition and thyroid hormone effectively maintained antioxidant status to overcome this situation in teleosts.

Keywords: Anabas testudineus, Antioxidant enzyme, Fish, Reactive oxygen species, Superoxide dismutase, Thyroid hormone

*E-mail: oommenvo@yahoo.com

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 45, December 2008, pp. 399-403

 

 

Effect of Na+ ions on pH-dependent conformational changes in brush border sucrase-isomaltase in mice intestine

Shiffalli Gupta1, Safrun Mahmood2, Rizwan Hasan Khan3 and Akhtar Mahmood1*

1Department of Biochemistry, Panjab University, Chandigarh 160014; 2Department of Biotechnology and Experimental Medicine, Post-graduate Institute of Medical Education and Research, Chandigarh 160012; 3Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India

Received 20 April 2008; revised 12 August 2008

Intestinal brush border sucrase-isomaltase (sucrose D-glucosidase E.C. 3.2.1.48, E.C. 3.2.1.10) exhibits pH-dependent stimulatory or inhibitory effects in response to Na+ ions. However, whether the enzyme undergoes conformational modulations as a function of pH and in the presence of alkali metal ions is not known. In this paper, we investigated the structural and functional relationship of purified murine sucrase in response to pH and Na+ ions using UV-CD fluorescence and spectroscopic studies. Kinetic studies revealed that at pH 5.0, the enzyme activation by Na+ ions was V-type, which changed to K-type at pH 7.2, whereas at alkaline pH (8.5), Na+ ions inhibited the enzyme activity and inhibition was uncompetitive in nature, affecting both the Km and Vmax components. Far UV-CD spectra of protein at pH 7.2 in the absence and presence of Na+ were almost overlapping, suggesting that secondary structure of protein was not affected upon addition of the salt. However, near UV-CD spectra indicated marked alterations in the tertiary structure of protein in presence of  50 mM Na+ ions. Increase in pH from 7.2 to 8.5 resulted in a marked rise in fluorescence intensity and red shift in λmax due to tryptophan residues in the enzyme molecule. These findings suggested that alterations in enzyme activity as a function of pH and Na+ ions was associated with ionization of key amino acid residues together with structural modifications in the enzyme conformation around neutral or alkaline pH.

Keywords: Brush border sucrase, Mice intestine, Alkali metal ions, pH-dependent effects, UV-CD spectral analysis, Fluorescence spectral studies

*E-mail: akhtarmah@yahoo.com

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 45, December 2008, pp. 404-409

 

 

Optimization of extracellular xylanase production by Sclerotinia sclerotiorum S2 using factorial design

Olfa Ellouze*, Sami Fattouch, Faouzi Mestiri, Mohamed Radhouen Aniba and Mohamed Nejib Marzouki

Biological Engineering unit, National Institute of Applied Sciences and Technology (I.N.S.A.T.) BP 676, 1080 Tunis Cedex, Tunisia

Received 30 January 2008; revised 19 September 2008

The improvement of xylanase production by Sclerotinia sclerotiorum S2 using a liquid fermentation culture was investigated. The optimized process was divided into three basic steps: (i) evaluating xylanase inducers using different agricultural residues such as wheat bran, oat bran, orange peel and barley bran at 1% final concentration, and also filter paper. Among these, wheat bran showed the maximum activity (2.5 U/ml) at 12 days post-inoculation; (ii) for optimization, we determined the optimal concentration of inducer, the effect of phosphate anion (K2HPO4/KH2PO4) and culture aeration using a rotary shaker at 100 and 180 rpm. The optimal conditions for these three factors were determined in an experimental panel using factorial data, in which a mathematical model (Minitab software) was fitted; (iii) The optimized culture medium containing a high level of wheat bran (3%) without KH2PO4-K2HPO4 and submitted to a high agitation (180 rpm/min) increased the xylanase production from 2.5 U/ml to 4 U/ml (1.6-fold).

Keywords: Xylanase production; Sclerotinia sclerotiorum S2; Factorial design; Wheat bran; Culture aeration; Phosphate anion effect.

*E-mail: Ellouze_olfa@yahoo.fr

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 45, December 2008, pp. 410-415

 

 

Dietary cholesterol and estrogen administration elevate brain apolipoprotein E in mice by different mechanisms#

Neelam Srivastava, Maurizio Averna1 and Rai Ajit K Srivastava*

Clonegen Biotechnology, USA, Clonegen Biotechnology Pvt Ltd, Noida, India; 1University of Palermo, Palermo, Italy

Received 12 January 2008; revised 23 Octoberr 2008

Apolipoprotein (apo) E plays an important role in the whole body cholesterol homeostasis. Recent studies suggest that it may also be involved in the local cholesterol transport in the brain, and influence the pathogenesis of Alzheimer’s disease (AD) by interacting with the b-amyloid protein and brain lipoprotein receptors. Since apoE expression is highest in the brain, next only to the liver and associated with the pathogenesis of AD, we hypothesized that dietary and hormonal intervention, known to regulate hepatic apoE expression may also regulate brain apoE and thereby influence local cholesterol transport. To test this hypothesis, groups of male C57BL mice were fed either regular rodent chow or high fat (HF) and high cholesterol enriched diet for 3 weeks. In a separate study, groups of male mice were administered pharmacological doses of 17-b estradiol for 5 consecutive days and sacrificed on the 6th day. As expected, HF diet elevated liver apoE mRNA and apoE synthesis. Similar to liver, brain apoE mRNA and synthesis also increased, following HF feeding. Estradiol administration increased liver apoE synthesis without affecting apoE mRNA. Interestingly, estradiol administration also increased the brain apoE synthesis, but without altering the brain apoE mRNA. These studies suggested that dietary cholesterol and estrogen administration elevated the brain apoE by different mechanisms.

Keywords: Apolipoprotein E, Brain, Cholesterol, Alzheimer’s disease, Estrogen, Mouse

*E-mail: ajitsriva@gmail.com

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 45, December 2008, pp. 416-420

 

 

Semi-empirical calculations on paullones, a promising class of cyclin-dependent kinase inhibitors

Pooja Sapra Sharma*, Reena Tyagi & Rajan Sharma

Department of Chemistry, C.S.S.S. (PG) College, Machhra, Meerut, 250003, Uttar Pradesh, India

Received 26 February 2008; revised 18 August 2008

Paullones, a group of ATP-competitive 7,12-dihydroindolo [3,2-d][1]benzazepin-6(5H)-ones are well-established cyclin-dependent kinase (CDK) inhibitors with promising anti-tumoral properties. In this study, AM1 (Austin model 1) calculations have been performed on paullones and their biological activities have been explained with respect to their HOMO and LUMO energies. 9-Substituted paullones with electrophilic character show high potencies. The interaction of 9th substitutent with Phe-80 of receptor plays a key role in binding and potency. The study will help medicinal chemists to design efficient CDK inhibitiors.

Keywords: Paullone, Cyclin-dependent kinase inhibitor, Austin model 1, Highest occupied molecular orbital, Lowest unoccupied molecular orbital

E-mail: poojasapra.sharma@gmail.com

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 45, December 2008, pp. 421-429

 

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

G K Solanki, Anand Amin, Ashvin Padhiyar, A K Ray+ and A T Oza*

Department of Physics, + Department of Chemistry, Sardar Patel University, Vallabh Vidyanagar, -388 120, Gujarat, India

 

Received 31 January 2008; revised 14 October 2008

The solid state spectroscopy of charge transfer complexes of biomolecules such as fatty acids, tripalmitin, lysozyme. folic acid, β-carotene, cytochrome c, valinomycin and gramicidin has been carried out. The absorption coefficient is related with electronic conductivity. A half-power beta density is found common among these macromolecular solids, indicating photon-induced polaron hopping or hopping of a charge carrier between two branches of a polariton. Band gap vs full width at half-maximum of the mid-IR peak also reveals a linear relation.

Keywords: Polaron hopping, Beta density, Infrared spectra, Biomolecular solids, Charge transfer complexes

E-mail: ajayozat@yahoo.com

 

 

Indian Journal of Biochemistry & Biophysics

Vol. 45, December 2008, pp.430-432

 

 

TRendys Meeting Report 2008


TRendys in Biochemistry’, since 1993, has been serving as a platform for discussions on latest research interests in biochemistry and molecular biology that have the potential to open new horizons in future research. The 14th Annual Meeting, TRendys-2008 was held at the Indian Institute of Science (IISc), Bangalore (Nov. 14th-15th 2008). Many enthusiastic students and scientists from various institutes and universities in Bangalore attended the conference. In the welcome address, Prof. H S Savithri (Department of Biochemistry, Indian Institute of Science) mentioned that the TRendys at IISc coincided with the centenary year of the institute. Prof. K Subba Rao, National Convener, TRendys in Biochemistry, presented the objectives and history of the TRendys. Prof. T Ramasarma, who originally conceived this forum, explained how Trendy’s has grown in years and now reaches students at a national level.

The TRendys-2008 Oration award was conferred to Prof. P Balaram, Director, Indian Institute of Science. In his talk, titled as “Can enzymes be Designed or Redesigned?” he demonstrated how after decades of study of protein biochemistry and structure, we still have not mastered it enough to customize it. The enzymes are known to catalyze reactions with utmost selectivity and stereo specificity. However, researchers have attempted to design catalytic motifs of the enzymes on other structurally unrelated non-enzymatic proteins. Computational methods have been used which predict rational mutations that can bring about such convergent evolution. Therefore, one of the enzymes used for such purposes is triose phosphate isomerase (TIM), for which there is a large number of sequences and structures in the database. However, as Prof. Balaram presented in his talk, such attempts have made news often, but seem to be making false promises as indicated by the number of retractions reported. He concluded in his talk that we still need insights into the protein structure and enzyme catalysis in order to attempt at rational engineering of the enzymes.

Dr. Renee M Borges (Centre for Ecological Sciences, Indian Institute of Science) presented recent advances in the chemical communicative ecology. For defense against insect herbivores, plants are known to use specific elicitors present in insect oral secretions such as fatty acid-amino acid conjugates (FACs). In response to FACs, plants may release volatile organic compounds (VOCs) that can trigger defense in farther parts of the same plant or even in different plants. VOCs may also attract parasitoids of these insect herbivores. Apart for defense purposes, plants are also capable of switching on and off the signals for attracting generalized or specialist insect pollinators.

Dr. Nagasuma Chandra (Bioinformatics Centre & Supercomputer Education and Research Centre, Indian Institute of Science) gave an exhaustive description about the systems biology and its application in drug discovery and medicine. Systems biology integrates data from high throughput experiments with mathematical and computational analysis to describe the interactions between components of a biological system, in order to explain the function and behavior of that system. In drug discovery, systems biology can predict rational drug targets, while considering aspects like their metabolic importance, adverse effects, drug resistance and drug toxicity. In the field of medicine, it may play crucial role in diagnosis, treatment and prevention.

Dr. Lalitha Gowda presented the potentialities in nutrigenomics; the culmination of diet, health and genomics. The talk focused on the effect of dietary and bioactive food components at genetic, epigenetic and proteomic levels. Some of the dietary components can confer prevention against certain diseases by altering molecular mechanisms. For example, the dietary oils can effect the gene expression of HMG-CoA, LDL receptor, sterol regulatory element-binding protein and cholesterol 7a-hydroxylase, and thus affect the body cholesterol levels. Various techniques useful for analysis in nutrigenomics were discussed.

Dr. Ram Rajasekharan (Department of Biochemistry, Indian Institute of Science) in his inspiring talk described the cytosolic triacylglcerol (TAG) biosynthetic machinery from the oleaginous yeast, and the monoacylglycerol (MAG) pathway present in the soluble fraction for TAG biosynthesis in developing oilseeds, both of which have been discovered in his laboratory. He illustrated how discoveries in basic research can be used in conjunction with the industry to develop new applications.

Dr. Polani B Sheshagiri (Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science) described the molecular mechanisms of gametogenesis, fertilization, sperm capacitation, blastocyst hatching and early embryonic patterning. He also described the implications of this study on the development in the field of in vitro fertilization, infertility and developmental defects.

Dr. R V Omkumar (Department of Neurobiology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram) described the new biochemical basis for learning and memory. A form of synaptic plasticity called the long-term potentiation (LTP) provides a useful model to study the molecular and cellular mechanism of memory. He explained that calcium/calmodulin-dependent protein kinase II, a protein involved in LTP can stay persistently active by autophosphorylation for a few years even after the activating stimulus is removed. Also, it is not accessible to the enzyme machinery capable of deactivating it. These features attribute to its role as a memory molecule.

Dr. Sandhya Visweswariah (Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science) in her closing talk of the first day of conference discussed the role of cyclic nucleotides, such as cAMP and cGMP, as the secondary messengers in signaling pathways. The enzymes involved in maintaining the levels of these cyclic nucleotides are largely conserved across species. However, they may be regulated differently in different organisms. cAMP plays a crucial role in olfaction while, cGMP is essential for vision. The message is transmitted from these molecules mostly via G-protein-coupled proteins. The response may be activation or repression of downstream pathways, depending on whether Gs or Gi proteins are involved. Chorela and pertussis toxins are known to interfere with G-protein coupled pathways. Future aspects of research in this field were discussed.

Dr. Deepshikha Chakravorthy (Department of Microbiology & Cell Biology, Indian Institute of Science) presented the opening talk of the second day in which she mentioned that Salmonella typhi has now been studied for a hundred years. The existing vaccine that targets the Vi polysaccharide is effective against only 60% Salmonella strains. The pathogenicity islands (SPI1 to SPI5) acquired by Salmonella by horizontal transfer of genetic material make it a pathogen as against E. coli, with which it shares a common ancestry. SPI1 encodes for needle-like proteins essential for pathogenicity, and SPI2 encodes for intracellular proteins that helps the bacteria to survive in the nascent phagosome. Such proteins are essential for maintenance of the bacteria inside the cell during infection and can be potential candidates for vaccine development in future.

Dr. Udaykumar Ranga (Molecular Biology & Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research) discussed the current trends in HIV infection and treatment in his talk. The most promising treatment available at present is known as highly active anti-retroviral therapy (HAART). The new strategies being developed to counter the virus aim to eliminate the latent virus, unlike HAART that eliminates only the virus bulk. Strategies, such as using chromatin remodeling to modulate viral gene transcription, activate quiescent T-cell and thus the latent virus, or to suppress the latent virus without a possibility of activation are being considered for therapeutics.

Prof. Tapas Kumar Kundu (Molecular Biology & Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research) gave a lucid talk on the regulation of gene expression in humans by chromatin dynamics. There is a localization of nuclear processes, modulated by chromatin folding and unfolding which is a consequence of chromatin modifications, such as phosphorylation, acetylation, deacetylation and methylation. Dysfunction of proteins involved in chromatin remodeling, such as histone acetyltransferases (HATs) is responsible for several diseases. Potential use of HAT inhibitors such as anacardic acid, garcinol and LKT-14 in therapy was discussed.

Dr. Ajith V Kamath (Structural Biology, Jubliant Biosciences) presented the recent perspective for drug design used in the biotech industry. Kinases and phosphodiesterases have been implicated in many diseases and are thus promising drug targets. Proteins are crystallized with and/or without their substrates and inhibitors to study the interactions involved. Based on structural and kinetic analysis, leads are identified and followed up.

Dr. S Mahadevan (Department of Molecular Reproduction, Development and Genetics, Indian Institute of Science) commenced the “Spikes and Flashes” session with a talk on microbial biofilms. He discussed the role of quorum sensing in regulating gene expression in bacterial cells. Upon reaching a certain cell density, there is a division of labour in bacterial colonies. Differential gene expression in cells in the same colony enables them to perform separate functions of defense and metabolism. These observations may have significant implications in understanding their physiology and in planning defense strategies against pathogens.

Dr. M. Raghunath (National Institute of Nutrition, Hyderabad) discussed the role of Wnt/a-catenin pathway in the maternal obesity-induced adepogenesis and obesity in the later life of the offspring. Maternal under-nutrition for a variety of nutrients possibly programs obesity at the developmental level through the Wnt signaling pathway that is highly conserved during evolution. This could be a potential molecular target in alleviating the obesity epidemic.

Dr. Sathees C Raghavan (Department of Biochemistry, Indian Institute of Science) presented a review of cancer, its detection and therapy. Genetic aberrations such as mutations, deletions, duplications and translocations lead to various forms of cancer. The recent advances in use of chromosomal translocations as biomarkers for diagnosis, prognosis and therapy were discussed.

Dr. Anjali A Karande (Department of Biochemistry, Indian Institute of Science) spoke about the cancer immunotherapeutics in her talk. Conventional methods of therapy include chemotherapy and radiation treatment that kills fast dividing cells. However, the chemotherapeutic drugs may cause toxicity by killing normal fast dividing cells. Also, the patient is susceptible to relapse in the remission period. Immunotherapy during the remission period could prove to be effective against the cancer cells that escape the conventional treatment. Antibodies can be used for specific delivery of anti-cancer drugs to cancer cells, while sparing the normal cells from any damage.

The last session of the conference had talks in the field of biophysics. Dr. Siddartha P Sarma (Molecular Biophysics Unit, Indian Institute of Science) presented the history and principles of Nuclear Magnetic Resonance (NMR). He concluded his talk with examples of applications of this technique, like in structure determination, pKa determination, monitoring of biochemical reactions, kinetic analysis of the enzymes, protein-substrate interaction protein-protein interaction, in vivo NMR spectroscopy and tracing biosynthetic pathways.

The second talk in biophysics was by Dr. M R N Murthy (Molecular Biophysics Unit, Indian Institute of Science) who talked about structural aspects of viruses, virus-like particles and other non-structural viral proteins. The developments in molecular biology, X-ray crystallography and cryo-electron microscopy have provided tools to study the assembly and disassembly, and other aspects of viral life cycle, such as translation, replication and enzyme function. These could prove to be crucial for developing new drugs against viral infections.

Thus, the conference brought together the experts from various biological fields that brought forward the current trends of scientific research for the benefit of young students aspiring to be future scientists. The critical discussions after the talks were indicative of the interest and the fervor with which the students attended the conference. In the concluding session of the conference, three participating students summarized the talks presented in the two days of the conference.

 

Chhavi Mathur, Kalyani Josyula, Chetna Soni, Sudarshan Gadadhar, Ashwini S N, Sunitha N

Department of Biochemistry

Indian Institute of Science

Bangalore – 560 012


 

 

 

 

Annual Author Index

 

 


Ahmad A                                                                     379

Ahmad S                                                                      379

Ahmed R S                                                      7, 209, 278

Ali N                                                                              16

Amin A                                                                        421

Aniba M R                                                                   404

Aro E M                                                                      237

Aron M                                                                        116

Averna M                                                                    410

 

Baig M A                                                                     379

Banerjea A C                                                               141

Banerjee B D                                                   7, 209, 278

Bano A S                                                                     141

Baquer N Z                                                                    57

Bhan M K                                                                    219

Bhattacharya A                                                            209

Bishayi B                                                                     250

Brahmam A N                                                             341

Bulusu G                                                                     365

 

Canseven A G                                                             326

Chakraborti A                                                                  7

Chakravarty A K                                                           23

Chen Q X                                                                    184

Chi Z                                                                            174

Choi K J                                                                      374

Choi T                                                                          374

Choudhary N                                                               387

Chowdhury I                                                               289

Coskun S                                                                     326

 

Das D                                                                            98

De K                                                                            198

Devaky K S                                                                   51

Devi M M                                                                    263

Dimitrov M I                                                               310

Ding Y                                                                         374

Donchev A A                                                              310

Duddukuri G R                                                            341

Dutta A                                                                        192

Dutta N N                                                                    192

 

Eldin A A K                                                                332

Elgawad H M A                                                          332

Ellouze O                                                                     404

 

Fattouch S                                                                    404

 

Gao Z Q                                                                       244

Gayathri Elayidam U                                                   121

Getov V I                                                                     310

Ghosh K K                                                                  350

Gogoi S                                                                       192

Guha S                                                                           75

Gupta N P                                                                    116

Gupta S                                                                        399

 

Ha J                                                                             374

Han X                                                                          374

Haridas M                                                                      51

He S                                                                             174

Husain S                                                                      141

 

Ilaiyaraja N                                                                    30

Ismail M F                                                                     44

 

Jacob T                                                                          98

Jain S K                                                                         91

Jang M-S                                                                     305

Jebasingh T                                                                    98

 

Kandrač J                                                                     157

Kanervo E                                                                    237

Kanugula S                                                                  166

Kaul G                                                                         149

Kaur J                                                                          149

Khan R H                                                                    399

Khan S                                                                         141

Khushu S                                                                     263

Kim K-S                                                                      305

Kim S S                                                                       374

Koti J S                                                                        166

Krishnamoorthy P                                                       206

Krishnaswamy S                                                           98

Kumar H                                                                      141

Kumar P                                                                      263

Kumar R                                                                      116

Kumar R                                                                      116

Kumar V                                                                      269

 

Lee Jinhwa                                                                   374

Lee Jisum                                                                     374

Lee S                                                                            374

Lee Y-C                                                                       305

Leskovac V                                                                  157

Li J                                                                               174

Liang C W                                                                   275

Luo W C                                                                      184

 

Ma D Y                                                                       184

Mahmood A                                                                399

Mahmood S                                                                 399

Maithal K                                                                       91

Maitra S K                                                                   289

Malik J                                                                         219

Mandal N C                                                                 106

Mandal V                                                                     106

Mangrauthia S K                                                         387

Marzouki M N                                                             404

Meena K                                                                        91

Meng C X                                                                    244

Mestiri F                                                                      404

Minakshi                                                                      111

Mohanty P                                                                     37

Mukherjee R                                                                  91

Mukhopadhyay R                                                        250

Muraleedharen D                                                         121

Naik R M                                                                     126

Neogi U                                                                       141

Nirmal N                                                                      256

 

Om Praba G                                                                 256

Oommen O V                                                              393

Oza A T                                                                       421

 

Padh H                                                                           75

Padhiyar A                                                                   421

Pasha S T                                                                     209

Pathak D                                                                      116

Pathak M G                                                                 192

Pathak P P                                                                   269

Pathak R                                                                      278

Patra S                                                                         141

Peričin D                                                                      157

Popović M                                                                   157

Priya S                                                                         317

Pundir C S                                                                   111

 

Qin S                                                                           275

 

Raghavendra A S                                                           37

Raghupathy R                                                              229

Rajarani A P                                                                  30

Ramachandran V G                                                     141

Rana P                                                                         263

Rani N                                                                         141

Rao A                                                                          365

Rao D N                                                                      341

Rathore A                                                                    141

Ray A K                                                                      421

Ray P                                                                           219

Ray S                                                                           198

Riazunnisa K                                                                 37

Rima Dada                                                                   116

Roy K                                                                          198

Roychoudhury J                                                            16

 

Sadasivan C                                                                   51

Sadik N A H                                                                  44

Sai Krishna T                                                                 37

Sangeetha M                                                                206

Santha I M                                                                     30

Satoh K                                                                        237

Schansker G                                                                  37

Sen S K                                                                       106

Sengupta A                                                                  289

Sengupta C                                                                  198

Sengupta P                                                                     91

Seth V                                                                          209

Seyhan N                                                                     326

Shah M                                                                          98

Shaheen A A                                                         44, 332

Shamsi M B                                                                 116

Shankar V                                                                    141

Sharma D                                                                       57

Sharma P S                                                                  416

Sharma R                                                                     416

Sharma R K                                                                 116

Shehata N I                                                                  332

Singh G                                                                       116

Singh H                                                                       116

Singh K                                                                       116

Singh M                                                                       237

Sinha N                                                                          57

Solanki G K                                                                 421

Sood V                                                                        141

Sreejith P                                                                     393

Srinivasan R                                                                365

Srivastava N                                                                410

Srivastava Rai A K                                                      410

Strasser R J                                                                    37

Suchithra T V                                                              179

Sudhakaran P R                                                           317

Suke S G                                                                     278

Suke S G                                                                         7

Sunil B                                                                           37

Sunil Kumar P N                                                           51

Suryanarayana T                                                          166

 

Taha A                                                                           57

Tak E                                                                           374

Tanwar M                                                                    116

Tao X                                                                           305

Tian L                                                                          275

Toncheva-Panova T G                                                 310

Toromanov G R                                                           310

Tripathi A K                                                            7, 278

Trivić S                                                                        157

Tyagi A                                                                        387

Tyagi R                                                                        416

 

Usha R                                                                           98

 

Varshney A                                                                 263

Vats R P                                                                      269

Velmurugan D                                                             256

Venkatesh S                                                                 116

Verma S K                                                                   350

 

Wang G C                                                                    244

Wang L                                                                        174

Wang Q                                                                       184

Wang X                                                                       174

 

Xue C B                                                                       184

 

Yadav K D S                                                               345

Yadav R S S                                                                345

Yamamoto Y                                                               237

Yasmin H                                                                      23

Ye N H                                                                        244

Yeleswarapu S J                                                          365

Yu Z                                                                            305

 

Zhang X W                                                                  275

Zuhara K F                                                                  179


 

 

 

 

Annual Subject Index

 

 


A

 

Abscisic acid (ABA)

 

Cloning and characterization of drought responsive partial gene sequence(s) from Oryza sativa L. subsp. Indica

387

Acalyphin

 

Modeling studies on PLA2-inhibitor complexes

256

Acid phosphatase

 

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

Adriamycin (ADR)

 

Effect of probucol and desferroxamine against adriamycin toxicity in cardiac and renal tissues of rats

44

Aging

 

Exogenous administration of DHEA attenuates loss of SOD activity in the brain of old rats

57

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

AIDS

 

Host genes that affect progression of AIDS/HIV in India and novel gene therapeutic approaches against HIV

141

Alanine aminotransferase (ALT)

 

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

Alcohol inhibition

 

Porcine pancreas lipase catalyzed synthesis of lauryl laurate in organic solvent media

192

Alkali metal ions

 

Effect of Na+ ions on pH-dependent conformational changes in brush border sucrase-isomaltase in mice intestine

399

Alkyldimethyl ethanolammonium bromide

 

Kinetics of a-chymotrypsin catalyzed hydrolysis of 4-nitrophenyl acetate in ethanolamine surfactants

350

Alternative oxidase (AOX)

 

Mitochondrial complex I impairment and differential carbon monoxide sensitivity of cytochrome c oxidase in wild type and CMS II mutants of Nicotiana sylvestris

126

Alzheimer's disease (AD)

 

Dietary cholesterol and estrogen administration elevate brain apolipoprotein E in mice by different mechanisms

410

Aminolevulinate (ALA)

 

Localization of heme biosyntheis pathway enzymes in Plasmodium falciparum

365

Amphotericin B

 

Sodium stibogluconate: Therapeutic use in the management of leishmaniasis

16

α-Amylase (amyA)

 

Molecular cloning, expression and characterization of a-amylase gene from a marine bacterium Pseudoalteromonas sp. MY-1

305

Anabas testudineus

 

Tri-iodothyronine alters superoxide dismutase expression in a teleost Anabas testudineus

393

Antibacterial efficacy

 

Optimized culture conditions for bacteriocin production by Pediococcus acidilactici LAB 5 and its characterization

106

Antibodies

 

Natural immunity to rotavirus infection in children

219

Antigen presenting cells (APCs)

 

Natural immunity to rotavirus infection in children

219

Antigen, soluble

 

Effects of soluble antigen-induced immune cell activation on steroidogenesis in murine lymphoid organ

250

Antimonials, pentavalent

 

Sodium stibogluconate: Therapeutic use in the management of leishmaniasis

16

Antioxidants

 

Effects of various extremely low frequency magnetic fields on the free radical processes, natural antioxidant system and respiratory burst system activities in the heart and liver tissues

326

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

QSAR study of lipid peroxidation-inhibition potential of some phenolic antioxidants

198

Tri-iodothyronine alters superoxide dismutase expression in a teleost Anabas testudineus

393

Anti-viral therapy

 

Host genes that affect progression of AIDS/HIV in India and novel gene therapeutic approaches against HIV

141

Apicoplast

 

Localization of heme biosyntheis pathway enzymes in Plasmodium falciparum

365

Aplysulphurin 1

 

Modeling studies on PLA2-inhibitor complexes

256

Apolipoprotein (apo) E

 

Dietary cholesterol and estrogen administration elevate brain apolipoprotein E in mice by different mechanisms

410

Apoptosis

 

Activation of cell mediated immune response and apoptosis towards malignant cells with turmeric treatment in murine model

23

Curcumin-induced recovery from hepatic injury involves induction of apoptosis of activated hepatic stellate cells

317

Archaea, thermoacidophilic

 

DNA aggregation by an archaeal DNA binding protein Sac10b and its novel DNA nicking activity

166

Arylalkylamine N-acetyl transferase (AA-NAT)

 

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Arylamine glass beads

 

Co-immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase on to aryl amine glass beads affixed on plastic strip for determination of triglycerides in serum

111

Aspartate aminotransferase (AST)

 

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

Aspergillus flavus

 

Enzymatic characteristics of quercetinases from some indigenous Aspergillus flavus strains

345

Assisted reproduction technique (ART)

 

Genetic screening in couples experiencing recurrent assisted procreation failure

116

ATP hydrolysis

 

Protein translocation pathways across the inner and outer mitochondrial membranes

149

Austin model 1

 

Semi-empirical calculations on paullones, a promising class of cyclin-dependent kinase inhibitors

416

Autoimmunity

 

Xenobiotic-induced immune alterations: Implications in health and disease

7

AZF

 

Genetic screening in couples experiencing recurrent assisted procreation failure

116

 

 

B

 

Bacteriocin

 

Optimized culture conditions for bacteriocin production by Pediococcus acidilactici LAB 5 and its characterization

106

Beta density

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

Biological rhythm

 

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Blood sugar

 

Myeloperoxidase activity in infection complicated and uninfected diabetic patients

179

Bond dissociation enthalpy (BDE)

 

QSAR study of lipid peroxidation-inhibition potential of some phenolic antioxidants

198

Brain

 

Dietary cholesterol and estrogen administration elevate brain apolipoprotein E in mice by different mechanisms

410

Exogenous administration of DHEA attenuates loss of SOD activity in the brain of old rats

57

Brassica juncea

 

Cloning and characterization of DGAT cDNA sequence from Brassica juncea cv. Pusa Bold

30

Brush border sucrase

 

Effect of Na+ ions on pH-dependent conformational changes in brush border sucrase-isomaltase in mice intestine

399

 

 

C

 

Calvin cycle

 

Thermodynamic properties of the Calvin cycle and pentose phosphate pathway

157

Cancer

 

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Carbon monoxide sensitivity

 

Mitochondrial complex I impairment and differential carbon monoxide sensitivity of cytochrome c oxidase in wild type and CMS II mutants of Nicotiana sylvestris

126

Cardamom mosaic virus (CdMV)

 

Optimized expression, solubilization and purification of nuclear inclusion protein b of cardamom mosaic virus

98

Cardiolipin

 

Pichia pastoris INO1 gene expression affects intracellular maltase activity and MAL1+ gene expression in Schizosaccharomyces pombe

174

b-Carotene

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

Carotenoids

 

Functional characterization of sll0659 from Synechocystis sp. PCC 6803

275

Catalase (CAT)

 

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

Cathepsins

 

as fundamental effectors of endolysosomal proteolysis

75

CCR5

 

Host genes that affect progression of AIDS/HIV in India and novel gene therapeutic approaches against HIV

141

Cell division

 

Functional characterization of sll0659 from Synechocystis sp. PCC 6803

275

Cellular immunity

 

Activation of cell mediated immune response and apoptosis towards malignant cells with turmeric treatment in murine model

23

Chaperone activity

 

A new method for purification of functional recombinant GST-cyclophilin A protein from E. coli

374

Charge transfer complexes

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

Chemokine receptor

 

Host genes that affect progression of AIDS/HIV in India and novel gene therapeutic approaches against HIV

141

Children

 

Natural immunity to rotavirus infection in children

219

Chlorogenic acid

 

Modeling studies on PLA2-inhibitor complexes

256

Chlorophyll

 

Application of fast chlorophyll a fluorescence transient (OJIP) analysis to monitor functional integrity of pea (Pisum sativum) mesophyll protoplasts during isolation

37

In vivo quality control of photosystem II in cyanobacteria Synechocystis sp. PCC 6803: D1 protein degradation and repair under the influence of light, heat and darkness

237

Cholesterol

 

Dietary cholesterol and estrogen administration elevate brain apolipoprotein E in mice by different mechanisms

410

Chromatofocusing

 

Plastocyanin microheterogeneity in Scenedesmus acutus MT8

310

Chromosomal abnormalities

 

Genetic screening in couples experiencing recurrent assisted procreation failure

116

a-Chymotrypsin (a-CT)

 

Kinetics of a-chymotrypsin catalyzed hydrolysis of 4-nitrophenyl acetate in ethanolamine surfactants

350

Cloning

 

Cloning and characterization of DGAT cDNA sequence from Brassica juncea cv. Pusa Bold

30

Cloning and characterization of drought responsive partial gene sequence(s) from Oryza sativa L. subsp. Indica

387

Molecular cloning, expression and characterization of a-amylase gene from a marine bacterium Pseudoalteromonas sp. MY-1

305

Optimized conditions for high-level expression and purification of recombinant human interleukin-2 in E. coli

91

Co-immobilization

 

Co-immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase on to aryl amine glass beads affixed on plastic strip for determination of triglycerides in serum

111

Coproporphyrinogen

 

Localization of heme biosyntheis pathway enzymes in Plasmodium falciparum

365

Corticosterone

 

Effects of soluble antigen-induced immune cell activation on steroidogenesis in murine lymphoid organ

250

Cotton

 

Identification and partial characterization of juvenile hormone esterase from cotton pest Dysdercus cingulatus

121

Curcuma longa Linn.

 

Activation of cell mediated immune response and apoptosis towards malignant cells with turmeric treatment in murine model

23

Curcumin-induced recovery from hepatic injury involves induction of apoptosis of activated hepatic stellate cells

317

Curcumin

 

Activation of cell mediated immune response and apoptosis towards malignant cells with turmeric treatment in murine model

23

Curcumin-induced recovery from hepatic injury involves induction of apoptosis of activated hepatic stellate cells

317

Modeling studies on PLA2-inhibitor complexes

256

Cyanobacteria

 

In vivo quality control of photosystem II in cyanobacteria Synechocystis sp. PCC 6803: D1 protein degradation and repair under the influence of light, heat and darkness

237

Cyclin-dependent kinase (CDK) inhibitor

 

Semi-empirical calculations on paullones, a promising class of cyclin-dependent kinase inhibitors

416

Cyclophilin A (CypA)

 

A new method for purification of functional recombinant GST-cyclophilin A protein from E. coli

374

Cystibiotics

 

Optimized culture conditions for bacteriocin production by Pediococcus acidilactici LAB 5 and its characterization

106

Cytochrome c

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

Cytochrome oxidase

 

Mitochondrial complex I impairment and differential carbon monoxide sensitivity of cytochrome c oxidase in wild type and CMS II mutants of Nicotiana sylvestris

126

Cytokines

 

Alterations in immunoglobulins and cytokine levels in blood of malathion poisoning cases

209

Effects of soluble antigen-induced immune cell activation on steroidogenesis in murine lymphoid organ

250

Manipulation of cytokine production profiles as a therapeutic approach for immunologic pregnancy loss

229

Melatonin treatment prevents modulation of cell-mediated immune response induced by propoxur in rats

278

Natural immunity to rotavirus infection in children

219

Optimized conditions for high-level expression and purification of recombinant human interleukin-2 in E. coli

91

Cytotoxic T lymphocytes (CTLs)

 

Activation of cell mediated immune response and apoptosis towards malignant cells with turmeric treatment in murine model

23

 

 

D

 

DBNP (DNA binding nucleoid protein)

 

DNA aggregation by an archaeal DNA binding protein Sac10b and its novel DNA nicking activity

166

D1 degradation

 

In vivo quality control of photosystem II in cyanobacteria Synechocystis sp. PCC 6803: D1 protein degradation and repair under the influence of light, heat and darkness

237

Dehydroepiandrosterone (DHEA)

 

Effects of soluble antigen-induced immune cell activation on steroidogenesis in murine lymphoid organ

250

Exogenous administration of DHEA attenuates loss of SOD activity in the brain of old rats

57

Desferroxamine (DFO)

 

Effect of probucol and desferroxamine against adriamycin toxicity in cardiac and renal tissues of rats

44

Diabetes mellitus (DM)

 

Myeloperoxidase activity in infection complicated and uninfected diabetic patients

179

Diacylglycerol acyltransferase (DGAT)

 

Cloning and characterization of DGAT cDNA sequence from Brassica juncea cv. Pusa Bold

30

Diarrhea

 

Natural immunity to rotavirus infection in children

219

2, 6-Dichloroindophenol (DCIP)

 

Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis

244

Diclofenac (DC)

 

Protective effect of taurine and quercetin against renal dysfunction associated with the combined use of gentamycin and diclofenac

332

Differential expression

 

Cloning and characterization of drought responsive partial gene sequence(s) from Oryza sativa L. subsp. Indica

387

Dimorphism

 

Plastocyanin microheterogeneity in Scenedesmus acutus MT8

310

Dioxygenase

 

Enzymatic characteristics of quercetinases from some indigenous Aspergillus flavus strains

345

Diphenolase

 

Inhibitory effects of 2-hydroxybenzaldehyde on the activity of phenoloxidase from Pieris rapae larvae

184

DNA

 

aggregation

 

DNA aggregation by an archaeal DNA binding protein Sac10b and its novel DNA nicking activity

166

isolation

 

Cloning and characterization of DGAT cDNA sequence from Brassica juncea cv. Pusa Bold

30

nicking

 

DNA aggregation by an archaeal DNA binding protein Sac10b and its novel DNA nicking activity

166

DNA-enzymes

 

Host genes that affect progression of AIDS/HIV in India and novel gene therapeutic approaches against HIV

141

Doxorubicin hydrochloride. see Adriamycin (ADR)

 

Drought

 

Cloning and characterization of drought responsive partial gene sequence(s) from Oryza sativa L. subsp. Indica

387

Dydrogesterone

 

Manipulation of cytokine production profiles as a therapeutic approach for immunologic pregnancy loss

229

Dysdercus cingulatus

 

Identification and partial characterization of juvenile hormone esterase from cotton pest Dysdercus cingulatus

121

 

 

E

 

Electromagnetic fields (EMF)

 

Effects of various extremely low frequency magnetic fields on the free radical processes, natural antioxidant system and respiratory burst system activities in the heart and liver tissues

326

Electromagnetic waves (EMW)

 

Harmful effects of 41 and 202 MHz radiations on some body parts and tissues

269

Endosomes

 

Cathepsins: Fundamental effectors of endolysosomal proteolysis

75

Environment

 

Cloning and characterization of drought responsive partial gene sequence(s) from Oryza sativa L. subsp. Indica

387

Xenobiotic-induced immune alterations: Implications in health and disease

7

Enzyme immobilization

 

Co-immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase on to aryl amine glass beads affixed on plastic strip for determination of triglycerides in serum

111

Estrogen

 

Dietary cholesterol and estrogen administration elevate brain apolipoprotein E in mice by different mechanisms

410

Ethanolic turmeric extract (ETE)

 

Activation of cell mediated immune response and apoptosis towards malignant cells with turmeric treatment in murine model

23

Expression

 

Molecular cloning, expression and characterization of a-amylase gene from a marine bacterium Pseudoalteromonas sp. MY-1

305

Optimized conditions for high-level expression and purification of recombinant human interleukin-2 in E. coli

91

Optimized expression, solubilization and purification of nuclear inclusion protein b of cardamom mosaic virus

98

Pichia pastoris INO1 gene expression affects intracellular maltase activity and MAL1+ gene expression in Schizosaccharomyces pombe

174

Expression, differential

 

Cloning and characterization of drought responsive partial gene sequence(s) from Oryza sativa L. subsp. Indica

387

 

 

F

 

FA-MLR (Multiple linear regression with factor analysis)

 

QSAR study of lipid peroxidation-inhibition potential of some phenolic antioxidants

198

Flourescence activated cell sorter (FACS)

 

Activation of cell mediated immune response and apoptosis towards malignant cells with turmeric treatment in murine model

23

Fluorescence (Chl a)

 

Application of fast chlorophyll a fluorescence transient (OJIP) analysis to monitor functional integrity of pea (Pisum sativum) mesophyll protoplasts during isolation

37

Folic acid

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

Food spoilage

 

Optimized culture conditions for bacteriocin production by Pediococcus acidilactici LAB 5 and its characterization

106

Formic acid

 

A new method for purification of functional recombinant GST-cyclophilin A protein from E. coli

374

Free radicals

 

Effects of various extremely low frequency magnetic fields on the free radical processes, natural antioxidant system and respiratory burst system activities in the heart and liver tissues

326

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Fungal strains

 

Enzymatic characteristics of quercetinases from some indigenous Aspergillus flavus strains

345

 

 

G

 

Gender

 

Non-invasive assessment of oxidative capacity in young Indian men and women

263

Gene expression

 

Molecular cloning, expression and characterization of a-amylase gene from a marine bacterium Pseudoalteromonas sp. MY-1

305

Optimized conditions for high-level expression and purification of recombinant human interleukin-2 in E. coli

91

Optimized expression, solubilization and purification of nuclear inclusion protein b of cardamom mosaic virus

98

Pichia pastoris INO1 gene expression affects intracellular maltase activity and MAL1+ gene expression in Schizosaccharomyces pombe

174

Gene sequencing

 

Cloning and characterization of DGAT cDNA sequence from Brassica juncea cv. Pusa Bold

30

Gentamycin

 

Protective effect of taurine and quercetin against renal dysfunction associated with the combined use of gentamycin and diclofenac

332

Gibbs free energy

 

Thermodynamic properties of the Calvin cycle and pentose phosphate pathway

157

Glucocorticoid (GC)

 

Effects of soluble antigen-induced immune cell activation on steroidogenesis in murine lymphoid organ

250

Glucose repression

 

Pichia pastoris INO1 gene expression affects intracellular maltase activity and MAL1+ gene expression in Schizosaccharomyces pombe

174

Gluthatione-S-transferase (GST) fusion protein

 

A new method for purification of functional recombinant GST-cyclophilin A protein from E. coli

374

Glycerol kinase (GK)

 

Co-immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase on to aryl amine glass beads affixed on plastic strip for determination of triglycerides in serum

111

Glycerol-3-phosphate oxidase (GPO)

 

Co-immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase on to aryl amine glass beads affixed on plastic strip for determination of triglycerides in serum

111

Gracilin A

 

Modeling studies on PLA2-inhibitor complexes

256

Gramicidin

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

 

 

H

 

Health

 

Xenobiotic-induced immune alterations: Implications in health and disease

7

Heart

 

Effect of probucol and desferroxamine against adriamycin toxicity in cardiac and renal tissues of rats

44

Heat-shock protein (HSPs)

 

Localization of heme biosyntheis pathway enzymes in Plasmodium falciparum

365

Heavy metals

 

Xenobiotic-induced immune alterations: Implications in health and disease

7

Heme

 

Purification and characterization of sulfite oxidase from goat liver

379

biosynthesis

 

Localization of heme biosyntheis pathway enzymes in Plasmodium falciparum

365

Hepatic fibrosis

 

Curcumin-induced recovery from hepatic injury involves induction of apoptosis of activated hepatic stellate cells

317

Hepatic stellate cells (HSCs)

 

Curcumin-induced recovery from hepatic injury involves induction of apoptosis of activated hepatic stellate cells

317

Hepatotoxicity

 

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

Highest occupied molecular orbital (HOMO)

 

Semi-empirical calculations on paullones, a promising class of cyclin-dependent kinase inhibitors

416

HIV (Human immunodeficiency virus)

 

Host genes that affect progression of AIDS/HIV in India and novel gene therapeutic approaches against HIV

141

Hormones

 

Effects of soluble antigen-induced immune cell activation on steroidogenesis in murine lymphoid organ

250

Juvenile hormone esterase (JHE)

 

Identification and partial characterization of juvenile hormone esterase from cotton pest Dysdercus cingulatus

121

HSNP (helix-stabilizing nucleoid proteins)

 

DNA aggregation by an archaeal DNA binding protein Sac10b and its novel DNA nicking activity

166

Human lactoferrin (HLf)

 

Conformation of a residue substituted fragment (349-364) of human lactoferrin protein in DMSO-d6 by 1H NMR and restrained molecular dynamics

51

2-Hydroxybenzaldehyde (2-HBD)

 

Inhibitory effects of 2-hydroxybenzaldehyde on the activity of phenoloxidase from Pieris rapae larvae

184

Hydroxyindole-O-methyl transferase (HIOMT)

 

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Hypothalamic pituitary adrenal (HPA)

 

Effects of soluble antigen-induced immune cell activation on steroidogenesis in murine lymphoid organ

250

 

 

I

 

Immune response

 

Natural immunity to rotavirus infection in children

219

IgE antibody response

 

Suppressive effect of Strychnos nux-vomica on induction of ovalbumin-specific IgE antibody response in mice

341

Melatonin treatment prevents modulation of cell-mediated immune response induced by propoxur in rats

278

Immune response activation

 

Activation of cell mediated immune response and apoptosis towards malignant cells with turmeric treatment in murine model

23

Immunoglobulins

 

Alterations in immunoglobulins and cytokine levels in blood of malathion poisoning cases

209

IgA/IgG

 

Natural immunity to rotavirus infection in children

219

Immunosuppression

 

Suppressive effect of Strychnos nux-vomica on induction of ovalbumin-specific IgE antibody response in mice

341

Immunotoxicity

 

Xenobiotic-induced immune alterations: Implications in health and disease

7

Inclusion bodies

 

Optimized conditions for high-level expression and purification of recombinant human interleukin-2 in E. coli

91

Optimized expression, solubilization and purification of nuclear inclusion protein b of cardamom mosaic virus

98

Infection bacterial

 

Optimized culture conditions for bacteriocin production by Pediococcus acidilactici LAB 5 and its characterization

106

natural

 

Natural immunity to rotavirus infection in children

219

Infertility

 

Genetic screening in couples experiencing recurrent assisted procreation failure

116

Inflammation

 

Modeling studies on PLA2-inhibitor complexes

256

Infrared (IR) spectra

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

Inhibition kinetics

 

Inhibitory effects of 2-hydroxybenzaldehyde on the activity of phenoloxidase from Pieris rapae larvae

184

Inner membrane (IM) proteins

 

Protein translocation pathways across the inner and outer mitochondrial membranes

149

INO1 gene expression

 

Pichia pastoris INO1 gene expression affects intracellular maltase activity and MAL1+ gene expression in Schizosaccharomyces pombe

174

Interferon-g (IFN-g)

 

Alterations in immunoglobulins and cytokine levels in blood of malathion poisoning cases

209

Effects of soluble antigen-induced immune cell activation on steroidogenesis in murine lymphoid organ

250

Manipulation of cytokine production profiles as a therapeutic approach for immunologic pregnancy loss

229

Melatonin treatment prevents modulation of cell-mediated immune response induced by propoxur in rats

278

Interleukins

 

Alterations in immunoglobulins and cytokine levels in blood of malathion poisoning cases

209

Manipulation of cytokine production profiles as a therapeutic approach for immunologic pregnancy loss

229

Optimized conditions for high-level expression and purification of recombinant human interleukin-2 in E. coli

91

Intermembrane space (IMS) proteins

 

Protein translocation pathways across the inner and outer mitochondrial membranes

149

Intracellular proteolysis

 

Cathepsins: Fundamental effectors of endolysosomal proteolysis

75

Intracytoplasmic sperm injection (ICSI)

 

Genetic screening in couples experiencing recurrent assisted procreation failure

116

Isoelectric focusing (IEF)

 

Plastocyanin microheterogeneity in Scenedesmus acutus MT8

310

 

 

J

 

Jet-lag

 

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Juvenile hormone esterase (JHE)

 

Identification and partial characterization of juvenile hormone esterase from cotton pest Dysdercus cingulatus

121

 

 

K

 

Kennedy pathway

 

Cloning and characterization of DGAT cDNA sequence from Brassica juncea cv. Pusa Bold

30

Kidney

 

Effect of probucol and desferroxamine against adriamycin toxicity in cardiac and renal tissues of rats

44

Protective effect of taurine and quercetin against renal dysfunction associated with the combined use of gentamycin and diclofenac

332

 

 

L

 

Lactate dehydrogenase (LDH)

 

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

Lactoferrin, human (HLf)

 

Conformation of a residue substituted fragment (349-364) of human lactoferrin protein in DMSO-d6 by 1H NMR and restrained molecular dynamics

51

Lauryl laurate

 

Porcine pancreas lipase catalyzed synthesis of lauryl laurate in organic solvent media

192

Learning

 

Exogenous administration of DHEA attenuates loss of SOD activity in the brain of old rats

57

Leishmania donovani

 

Sodium stibogluconate: Therapeutic use in the management of leishmaniasis

16

Leishmaniasis, visceral (VL)

 

Sodium stibogluconate: Therapeutic use in the management of leishmaniasis

16

Lentiviral vectors

 

Host genes that affect progression of AIDS/HIV in India and novel gene therapeutic approaches against HIV

141

Leucocyte migration inhibition (LMI)

 

Melatonin treatment prevents modulation of cell-mediated immune response induced by propoxur in rats

278

Lipase

 

Co-immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase on to aryl amine glass beads affixed on plastic strip for determination of triglycerides in serum

111

Porcine pancreas lipase catalyzed synthesis of lauryl laurate in organic solvent media

192

Lipid peroxidation (LPO)

 

Effects of various extremely low frequency magnetic fields on the free radical processes,natural antioxidant system and respiratory burst system activities in the heart and liver tissues

326

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

Protective effect of taurine and quercetin against renal dysfunction associated with the combined use of gentamycin and diclofenac

332

QSAR study of lipid peroxidation-inhibition potential of some phenolic antioxidants

198

Liver

 

Curcumin-induced recovery from hepatic injury involves induction of apoptosis of activated hepatic stellate cells

317

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

Purification and characterization of sulfite oxidase from goat liver

379

Lowest unoccupied molecular orbital (LUMO)

 

Semi-empirical calculations on paullones, a promising class of cyclin-dependent kinase inhibitors

416

Lycopene cyclase

 

Functional characterization of sll0659 from Synechocystis sp. PCC 6803

275

Lymphocytes

 

Activation of cell mediated immune response and apoptosis towards malignant cells with turmeric treatment in murine model

23

Alterations in immunoglobulins and cytokine levels in blood of malathion poisoning cases

209

Lymphoid organ

 

Effects of soluble antigen-induced immune cell activation on steroidogenesis in murine lymphoid organ

250

Lysozyme

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

 

 

M

 

Macrophage migration inhibition (MMI)

 

Melatonin treatment prevents modulation of cell-mediated immune response induced by propoxur in rats

278

Magnetic fields (MFs)

 

Effects of various extremely low frequency magnetic fields on the free radical processes, natural antioxidant system and respiratory burst system activities in the heart and liver tissues

326

Magnetic resonance spectroscopy (MRS)

 

Non-invasive assessment of oxidative capacity in young Indian men and women

263

Malaria

 

Localization of heme biosyntheis pathway enzymes in Plasmodium falciparum

365

Malathion

 

Alterations in immunoglobulins and cytokine levels in blood of malathion poisoning cases

209

MAL1+ gene expression

 

Pichia pastoris INO1 gene expression affects intracellular maltase activity and MAL1+ gene expression in Schizosaccharomyces pombe

174

Malondialdehyde (MDA)

 

Effect of probucol and desferroxamine against adriamycin toxicity in cardiac and renal tissues of rats

44

Effects of various extremely low frequency magnetic fields on the free radical processes, natural antioxidant system and respiratory burst system activities in the heart and liver tissues

326

Maltase activity

 

Pichia pastoris INO1 gene expression affects intracellular maltase activity and MAL1+ gene expression in Schizosaccharomyces pombe

174

Maltose-binding protein (MBP)

 

Optimized expression, solubilization and purification of nuclear inclusion protein b of cardamom mosaic virus

98

Marker enzymes

 

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

MAXDP (Maximal electrotopological positive variation)

 

QSAR study of lipid peroxidation-inhibition potential of some phenolic antioxidants

198

Melatonin (MT)

 

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Melatonin treatment prevents modulation of cell-mediated immune response induced by propoxur in rats

278

Membrane potential

 

Protein translocation pathways across the inner and outer mitochondrial membranes

149

Memory

 

Exogenous administration of DHEA attenuates loss of SOD activity in the brain of old rats

57

Mesophyll protoplasts

 

Application of fast chlorophyll a fluorescence transient (OJIP) analysis to monitor functional integrity of pea (Pisum sativum) mesophyll protoplasts during isolation

37

Micellar enzymology

 

Kinetics of a-chymotrypsin catalyzed hydrolysis of 4-nitrophenyl acetate in ethanolamine surfactants

350

Microheterogeneity

 

Plastocyanin microheterogeneity in Scenedesmus acutus MT8

310

Mitochondria

 

Localization of heme biosyntheis pathway enzymes in Plasmodium falciparum

365

Protein translocation pathways across the inner and outer mitochondrial membranes

149

Mitochondrial complex I

 

Mitochondrial complex I impairment and differential carbon monoxide sensitivity of cytochrome c oxidase in wild type and CMS II mutants of Nicotiana sylvestris

126

Mitochondrial precursor proteins

 

Protein translocation pathways across the inner and outer mitochondrial membranes

149

Molecular operating environment (MOE) software

 

Modeling studies on PLA2-inhibitor complexes

256

Molybdenum

 

Purification and characterization of sulfite oxidase from goat liver

379

Monocyte chemotactic protein 1 (MCP-1)

 

Effects of soluble antigen-induced immune cell activation on steroidogenesis in murine lymphoid organ

250

Monophenolase

 

Inhibitory effects of 2-hydroxybenzaldehyde on the activity of phenoloxidase from Pieris rapae larvae

184

Mustard. see Brassica juncea

 

Mutation

 

Mitochondrial complex I impairment and differential carbon monoxide sensitivity of cytochrome c oxidase in wild type and CMS II mutants of Nicotiana sylvestris

126

Myeloperoxidase (MPO)

 

Effects of various extremely low frequency magnetic fields on the free radical processes, natural antioxidant system and respiratory burst system activities in the heart and liver tissues

326

Myeloperoxidase activity in infection complicated and uninfected diabetic patients

179

 

 

N

 

N-acetyl- b-D-glucosaminidase (NAG)

 

Protective effect of taurine and quercetin against renal dysfunction associated with the combined use of gentamycin and diclofenac

332

N-acetyl-5-methoxytryptamine. see Melatonin

 

Natural infection

 

Natural immunity to rotavirus infection in children

219

Nephrotoxicity

 

Protective effect of taurine and quercetin against renal dysfunction associated with the combined use of gentamycin and diclofenac

332

Nicotiana sylvestris

 

Mitochondrial complex I impairment and differential carbon monoxide sensitivity of cytochrome c oxidase in wild type and CMS II mutants of Nicotiana sylvestris

126

Nitrate reductase induction

 

Mitochondrial complex I impairment and differential carbon monoxide sensitivity of cytochrome c oxidase in wild type and CMS II mutants of Nicotiana sylvestris

126

Nitric oxide (NO)

 

Effects of various extremely low frequency magnetic fields on the free radical processes, natural antioxidant system and respiratory burst system activities in the heart and liver tissues

326

4-Nitrophenyl acetate

 

Kinetics of a-chymotrypsin catalyzed hydrolysis of 4-nitrophenyl acetate in ethanolamine surfactants

350

NSAIDs (Non-steroidal anti-inflammatory drugs)

 

Protective effect of taurine and quercetin against renal dysfunction associated with the combined use of gentamycin and diclofenac

332

Nuclear encoded apicoplast targeted (NEAT) proteins

 

Localization of heme biosyntheis pathway enzymes in Plasmodium falciparum

365

Nuclear encoded mitochondrion targeted (NEMT) proteins

 

Localization of heme biosyntheis pathway enzymes in Plasmodium falciparum

365

Nuclear inclusion b (NIb)

 

Optimized expression, solubilization and purification of nuclear inclusion protein b of cardamom mosaic virus

98

Nuclear magnetic resonance (NMR)

 

Conformation of a residue substituted fragment (349-364) of human lactoferrin protein in DMSO-d6 by 1H NMR and restrained molecular dynamics

51

 

 

O

 

Oryza sativa L.

 

Cloning and characterization of drought responsive partial gene sequence(s) from Oryza sativa L. subsp. Indica

387

Oxidative capacity

 

Non-invasive assessment of oxidative capacity in young Indian men and women

263

Oxidative stress

 

Effect of probucol and desferroxamine against adriamycin toxicity in cardiac and renal tissues of rats

44

Effects of various extremely low frequency magnetic fields on the free radical processes, natural antioxidant system and respiratory burst system activities in the heart and liver tissues

326

Exogenous administration of DHEA attenuates loss of SOD activity in the brain of old rats

57

Protective effect of taurine and quercetin against renal dysfunction associated with the combined use of gentamycin and diclofenac

332

 

 

P

 

Passive cutaneous anaphylaxis (PCA)

 

Suppressive effect of Strychnos nux-vomica on induction of ovalbumin-specific IgE antibody response in mice

341

Paullone

 

Semi-empirical calculations on paullones, a promising class of cyclin-dependent kinase inhibitors

416

PCC6803

 

Functional characterization of sll0659 from Synechocystis sp. PCC 6803

275

Pea. see Pisum sativum

 

Pediococcus acidilactici

 

Optimized culture conditions for bacteriocin production by Pediococcus acidilactici LAB 5 and its characterization

106

Pentavalent antimonials

 

Sodium stibogluconate: Therapeutic use in the management of leishmaniasis

16

Pentose phosphate pathway

 

Thermodynamic properties of the Calvin cycle and pentose phosphate pathway

157

Peptides,  conformation/synthesis

 

Conformation of a residue substituted fragment (349-364) of human lactoferrin protein in DMSO-d6 by 1H NMR and restrained molecular dynamics

51

Polypeptide composition

 

Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis

244

Peroxidase (POD)

 

Co-immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase on to aryl amine glass beads affixed on plastic strip for determination of triglycerides in serum

111

Pesticides

 

Alterations in immunoglobulins and cytokine levels in blood of malathion poisoning cases

209

Xenobiotic-induced immune alterations: Implications in health and disease

7

Pests

 

Identification and partial characterization of juvenile hormone esterase from cotton pest Dysdercus cingulatus

121

Peyer’s patches (PP)

 

Natural immunity to rotavirus infection in children

219

Phenoloxidase (PO)

 

Inhibitory effects of 2-hydroxybenzaldehyde on the activity of phenoloxidase from Pieris rapae larvae

184

Phosphate anion effect

 

Optimization of extracellular xylanase production by Sclerotinia sclerotiorum S2 using factorial design

404

Phosphatidylinositol (PI)

 

Pichia pastoris INO1 gene expression affects intracellular maltase activity and MAL1+ gene expression in Schizosaccharomyces pombe

174

Phosphocreatine (PCr) recovery

 

Non-invasive assessment of oxidative capacity in young Indian men and women

263

Phospholipase A2 (PLA2)

 

Modeling studies on PLA2-inhibitor complexes

256

Photoinhibition

 

In vivo quality control of photosystem II in cyanobacteria Synechocystis sp. PCC 6803: D1 protein degradation and repair under the influence of light, heat and darkness

237

Photoreduction

 

Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis

244

Photosystem

 

Application of fast chlorophyll a fluorescence transient (OJIP) analysis to monitor functional integrity of pea (Pisum sativum) mesophyll protoplasts during isolation

37

Photosystem II (PSII)

 

Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis

244

In vivo quality control of photosystem II in cyanobacteria Synechocystis sp. PCC 6803: D1 protein degradation and repair under the influence of light, heat and darkness

237

Pichia pastoris

 

Pichia pastoris INO1 gene expression affects intracellular maltase activity and MAL1+ gene expression in Schizosaccharomyces pombe

174

Pieris rapae (L.)

 

Inhibitory effects of 2-hydroxybenzaldehyde on the activity of phenoloxidase from Pieris rapae larvae

184

Pigments

 

Functional characterization of sll0659 from Synechocystis sp. PCC 6803

275

Pineal gland

 

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Ping-Pong Bi-Bi

 

Porcine pancreas lipase catalyzed synthesis of lauryl laurate in organic solvent media

192

Pisum sativum

 

Application of fast chlorophyll a fluorescence transient (OJIP) analysis to monitor functional integrity of pea (Pisum sativum) mesophyll protoplasts during isolation

37

Plasmodium falciparum

 

Localization of heme biosyntheis pathway enzymes in Plasmodium falciparum

365

Plastocyanin (PC)

 

Plastocyanin microheterogeneity in Scenedesmus acutus MT8

310

Plastoquinone

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

Polymorphisms

 

Host genes that affect progression of AIDS/HIV in India and novel gene therapeutic approaches against HIV

141

Porcine pancreas lipase

 

Porcine pancreas lipase catalyzed synthesis of lauryl laurate in organic solvent media

192

Porphyra yezoensis

 

Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis

244

PPIase activity

 

A new method for purification of functional recombinant GST-cyclophilin A protein from E. coli

374

Pregnancy loss

 

Manipulation of cytokine production profiles as a therapeutic approach for immunologic pregnancy loss

229

Pre-term delivery (PTD)

 

Manipulation of cytokine production profiles as a therapeutic approach for immunologic pregnancy loss

229

Probucol (PROB)

 

Effect of probucol and desferroxamine against adriamycin toxicity in cardiac and renal tissues of rats

44

Progestogens

 

Manipulation of cytokine production profiles as a therapeutic approach for immunologic pregnancy loss

229

Propoxur

 

Melatonin treatment prevents modulation of cell-mediated immune response induced by propoxur in rats

278

Protease

 

In vivo quality control of photosystem II in cyanobacteria Synechocystis sp. PCC 6803: D1 protein degradation and repair under the influence of light, heat and darkness

 

Proteolysis, intracellular

 

Cathepsins: Fundamental effectors of endolysosomal proteolysis

75

Protoplast isolation

 

Application of fast chlorophyll a fluorescence transient (OJIP) analysis to monitor functional integrity of pea (Pisum sativum) mesophyll protoplasts during isolation

37

Pseudoalteromonas

 

Molecular cloning, expression and characterization of a-amylase gene from a marine bacterium Pseudoalteromonas sp. MY-1

305

Purification

 

A new method for purification of functional recombinant GST-cyclophilin A protein from E. coli

374

Optimized conditions for high-level expression and purification of recombinant human interleukin-2 in E. coli

91

Optimized expression, solubilization and purification of nuclear inclusion protein b of cardamom mosaic virus

98

Purification and characterization of sulfite oxidase from goat liver

379

 

 

Q

 

QSAR (Quantitative structure activity relationship)

 

QSAR study of lipid peroxidation-inhibition potential of some phenolic antioxidants

198

Semi-empirical calculations on paullones, a promising class of cyclin-dependent kinase inhibitors

416

Quercetin

 

Protective effect of taurine and quercetin against renal dysfunction associated with the combined use of gentamycin and diclofenac

332

Quercetinase

 

Enzymatic characteristics of quercetinases from some indigenous Aspergillus flavus strains

345

 

 

R

 

Radiation

 

Harmful effects of 41 and 202 MHz radiations on some body parts and tissues

269

Rapid eye movement (REM)

 

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Reactive oxygen species (ROS)

 

Effect of probucol and desferroxamine against adriamycin toxicity in cardiac and renal tissues of rats

44

Tri-iodothyronine alters superoxide dismutase expression in a teleost Anabas testudineus

393

Recurrent spontaneous miscarriage (RSM)

 

Manipulation of cytokine production profiles as a therapeutic approach for immunologic pregnancy loss

229

Reduced glutathione (GSH)

 

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

Refolding

 

Optimized conditions for high-level expression and purification of recombinant human interleukin-2 in E. coli

91

Renal blood flow (RBF)

 

Protective effect of taurine and quercetin against renal dysfunction associated with the combined use of gentamycin and diclofenac

332

Reproduction

 

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Yq microdeletion assisted

 

Genetic screening in couples experiencing recurrent assisted procreation failure

116

Retinol

 

Curcumin-induced recovery from hepatic injury involves induction of apoptosis of activated hepatic stellate cells

317

Ribozyme

 

Host genes that affect progression of AIDS/HIV in India and novel gene therapeutic approaches against HIV

141

Rice

 

Cloning and characterization of drought responsive partial gene sequence(s) from Oryza sativa L. subsp. Indica

387

 siRNA

 

Host genes that affect progression of AIDS/HIV in India and novel gene therapeutic approaches against HIV

141

RNA-dependent RNA polymerase (RdRP)

 

Optimized expression, solubilization and purification of nuclear inclusion protein b of cardamom mosaic virus

98

RNA isolation

 

Cloning and characterization of DGAT cDNA sequence from Brassica juncea cv. Pusa Bold

30

Rotavirus

 

Natural immunity to rotavirus infection in children

219

 

 

S

 

Sac10b

 

DNA aggregation by an archaeal DNA binding protein Sac10b and its novel DNA nicking activity

166

Scenedesmus acutus

 

Plastocyanin microheterogeneity in Scenedesmus acutus MT8

310

Schizosaccharomyces pombe

 

Pichia pastoris INO1 gene expression affects intracellular maltase activity and MAL1+ gene expression in Schizosaccharomyces pombe

174

Sclerotinia sclerotiorum

 

Optimization of extracellular xylanase production by Sclerotinia sclerotiorum S2 using factorial design

404

Seasonal affective disorder (SAD)

 

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Sequencing

 

Cloning and characterization of DGAT cDNA sequence from Brassica juncea cv. Pusa Bold

30

Severe combined immuno deficiency (SCID)

 

Natural immunity to rotavirus infection in children

219

Skeletal muscle

 

Non-invasive assessment of oxidative capacity in young Indian men and women

263

Sleep

 

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Sll0659

 

Functional characterization of sll0659 from Synechocystis sp. PCC 6803

275

Sodium nitrite

 

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

Sodium stibogluconate (SSG)

 

in therapeutic management of leishmaniasis

16

Solubilization

 

Optimized expression, solubilization and purification of nuclear inclusion protein b of cardamom mosaic virus

98

Specific absorption rate (SAR)

 

Harmful effects of 41 and 202 MHz radiations on some body parts and tissues

269

Spectroscopy

 

solid-state

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

UV-CD fluorescence

 

Effect of Na+ ions on pH-dependent conformational changes in brush border sucrase-isomaltase in mice intestine

399

Sporophytes, filamentous

 

Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis

244

Stearic acid

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

Steroidogenesis

 

Effects of soluble antigen-induced immune cell activation on steroidogenesis in murine lymphoid organ

250

Stigmasterol

 

Modeling studies on PLA2-inhibitor complexes

256

Stress

 

Cloning and characterization of drought responsive partial gene sequence(s) from Oryza sativa L. subsp. Indica

387

Xenobiotic-induced immune alterations: Implications in health and disease

7

Strychnos nux-vomica Linn

 

Suppressive effect of Strychnos nux-vomica on induction of ovalbumin-specific IgE antibody response in mice

341

Sucrase activity

 

Effect of Na+ ions on pH-dependent conformational changes in brush border sucrase-isomaltase in mice intestine

399

Sucrose density gradient ultracentrifugation (SDGUC)

 

Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis

244

Sulfite oxidase

 

Purification and characterization of sulfite oxidase from goat liver

379

Sulfolobus acidocaldarius

 

DNA aggregation by an archaeal DNA binding protein Sac10b and its novel DNA nicking activity

166

Superoxide dismutase (SOD)

 

Exogenous administration of DHEA attenuates loss of SOD activity in the brain of old rats

57

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

Tri-iodothyronine alters superoxide dismutase expression in a teleost Anabas testudineus

393

Suprachiasmatic nucleus (SCN)

 

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Surfactants

 

Kinetics of a-chymotrypsin catalyzed hydrolysis of 4-nitrophenyl acetate in ethanolamine surfactants

350

Synechocystis sp.

 

Functional characterization of sll0659 from Synechocystis sp. PCC 6803

275

In vivo quality control of photosystem II in cyanobacteria Synechocystis sp. PCC 6803: D1 protein degradation and repair under the influence of light, heat and darkness

237

 

 

T

 

Taurine

 

Protective effect of taurine and quercetin against renal dysfunction associated with the combined use of gentamycin and diclofenac

332

T cells

 

Natural immunity to rotavirus infection in children

219

T helper (Th) cells

 

Manipulation of cytokine production profiles as a therapeutic approach for immunologic pregnancy loss

229

Tectoridin

 

Modeling studies on PLA2-inhibitor complexes

256

Teleost

 

Tri-iodothyronine alters superoxide dismutase expression in a teleost Anabas testudineus

393

Thylakoid membranes

 

Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis

244

Thyroid hormone (TH)

 

Tri-iodothyronine alters superoxide dismutase expression in a teleost Anabas testudineus

393

Toxins

 

Xenobiotic-induced immune alterations: Implications in health and disease

7

Translocase of inner/outer membrane (TIM/TOM)

 

Localization of heme biosyntheis pathway enzymes in Plasmodium falciparum

365

Translocation

 

Protein translocation pathways across the inner and outer mitochondrial membranes

149

Triacylglycerol (TAG)

 

Cloning and characterization of DGAT cDNA sequence from Brassica juncea cv. Pusa Bold

30

Tricyclic antidepressants (TCA)

 

Melatonin: from discovery in bovine pineal gland to delineation of functions in human

289

Triglycerides (Tgs)

 

Co-immobilization of lipase, glycerol kinase, glycerol-3-phosphate oxidase and peroxidase on to aryl amine glass beads affixed on plastic strip for determination of triglycerides in serum

111

Tripalmitin

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

Tumor cells

 

Activation of cell mediated immune response and apoptosis towards malignant cells with turmeric treatment in murine model

23

Tumor necrosis factor-a (TNF-a)

 

Alterations in immunoglobulins and cytokine levels in blood of malathion poisoning cases

209

 

Effects of soluble antigen-induced immune cell activation on steroidogenesis in murine lymphoid organ

250

Manipulation of cytokine production profiles as a therapeutic approach for immunologic pregnancy loss

229

Melatonin treatment prevents modulation of cell-mediated immune response induced by propoxur in rats

278

Turmeric

 

Activation of cell mediated immune response and apoptosis towards malignant cells with turmeric treatment in murine model

23

Curcumin-induced recovery from hepatic injury involves induction of apoptosis of activated hepatic stellate cells

317

 

 

U

 

Ultracentrifugation (SDGUC)

 

Isolation and characterization of photosystem II from the filamentous sporophyte of Porphyra yezoensis

244

UV-CD fluorescence spectroscopy

 

Effect of Na+ ions on pH-dependent conformational changes in brush border sucrase-isomaltase in mice intestine

399

 

 

V

 

Valinomycin

 

Polaron hopping in some biomolecular solids and their charge transfer complexes

421

Viral protein (VP)

 

Natural immunity to rotavirus infection in children

219

Visceral leishmaniasis (VL)

 

Sodium stibogluconate: Therapeutic use in the management of leishmaniasis

16

Vitamin C

 

Hepatoprotective effect of vitamin C on sodium nitrite-induced lipid peroxidation in albino rats

206

 

 

W

 

Water deficit stress (WDS)

 

Cloning and characterization of drought responsive partial gene sequence(s) from Oryza sativa L. subsp. Indica

387

Wound healing

 

Myeloperoxidase activity in infection complicated and uninfected diabetic patients

179

 

 

X

 

Xenobiotics

 

Xenobiotic-induced immune alterations: Implications in health and disease

7

Xylanase production

 

Optimization of extracellular xylanase production by Sclerotinia sclerotiorum S2 using factorial design

404

 

 

Y

 

Yq microdeletion

 

Genetic screening in couples experiencing recurrent assisted procreation failure

116