Indian Journal of Chemical Technology

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VOLUME 15

NUMBER 6

NOVEMBER 2008

CODEN:ICHTEU

 

ISSN:0971-457X

 

CONTENTS 

Papers

 

 

Removal of fluoride from aqueous solution using graphite: A kinetic and thermo-

525

dynamic study

 

M Karthikeyan & K P Elango

 

 

Sorption of phenol from aqueous solution using activated carbon prepared from

533

Manilkara zapota seed

 

Kaushik Nath, Mehul Thummar, Mahesh Vaghela & Pranab Jani

 

 

Sorption studies on cresol red modified Amberlite IR400 (Cl-) resin: Binary and

541

selective separation of Hg2+ ions

 

Amjad Mumtaz Khan, Shalini Gautam & Sajad A Ganai

 

 

Miscibility of poly(methylmethacrelate) and cellulose acetate butyrate blends

547

in dimethyl formamide

 

M Selvakumar & D Krishna Bhat

 

 

Effect of screw speed and plasticizer on the torque requirement in single screw

555

extrusion of starch based plastics and their mechanical properties

 

Madhusweta Das

 

 

Effect of Na3PO4 on the corrosion inhibition efficiency of EDTA- Zn2+ system

560

for carbon steel in aqueous solution

 

T Umamathi, J Arockia Selvi, S Agnesia Kanimozhi, Susai Rajendran &

A John Amalraj

 

 

Catalytic conversion of propane to functionalized products

566

M Asadullah, M Shamim Uddin, M Israt Jahan, M Abdul Motin &

M Asaduzzaman

 

 

Application of monolithic stirred reactor in hydrogenation reaction

572

Chintan K Joshi & Parimal A Parikh

 

 

Zinc-poly(aniline) rechargeable battery assembled with aqueous electrolyte

576

Bharat Chandra Dalui, I N Basumallick & Susanta Ghosh

 

 

Development of Zn-Co alloy coatings by pulsed current from chloride bath

581

V Thangaraj, N K Udayashankar & A Chitharanjan Hegde

 

 

Bioleaching of low-grade copper ore using indigenous microorganisms

588

D Pradhan, S Pal, L B Sukla, G Roy Chaudhury & T Das

 

 

Voltammetric DNA biosensor for the study of mechanism of action of anticancer

593

drug-adriamycin

 

Sweety Tiwari & K S Pitre

 

 

Stability-indicating HPLC method for determination of vitamins B1, B2, B3

598

and B6 in pharmaceutical liquid dosage form

 

Shino Thomas, Rakesh Kumar, Ashutosh Sharma, Roshan Issarani & Badri

Prakash Nagori

 

 

Submerged membrane bioreactor system for municipal wastewater treatment

604

process: An overview

 

Neha Gupta, N Jana & C B Majumder

 

 

 

Notes

 

 

 

Synthesis, characterization and electrical conductivity of charge transfer

613

complex of p-toluidine and chloranil

 

Rakesh Sharma, Mukesh Paliwal, Sadhana Singh, Rameshwer Ameta &

Suresh C Ameta

 

 

Validated liquid chromatographic method for simultaneous estimation of

617

albendazole and ivermectin in tablet dosage form

 

Anil Waldia, Shubash Gupta, Roshan Issarani & Badri P Nagori

 

 

 

Acknowledgement to referees

621

 

 

Annual Author Index

 

623

Annual Keyword Index

 

626

Guidelines for Authors

631

 

 

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 525-532

 

 

Removal of fluoride from aqueous solution using graphite: A kinetic and thermodynamic study

 

M Karthikeyan & K P Elango*

 

Department of Chemistry, Gandhigram Rural University, Gandhigram 624 302, India

Email: drkpelango@rediffmail.com

 

Batch sorption system using various grades of graphite as adsorbents was investigated to remove fluoride ions from aqueous solutions. The system variables studied include initial concentration of the sorbate, agitation time, adsorbent dose, pH, co-ions and temperature. The experimental data fitted well to the Freundlich and Langmuir isotherms. Thermodynamic parameters such as DHo, DSo and DGo were calculated which indicated that the adsorption was endothermic and a physical process. Kinetic studies reveal that the adsorption follows reversible first order kinetics. XRD patterns of the adsorbent before and after adsorption were recorded to get a better in sight into the mechanism of the adsorption process.

Keywords: Adsorption, Fluoride, Graphite, Kinetics, Thermodynamics

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 533-540

 

 

Sorption of phenol from aqueous solution using activated carbon prepared from Manilkara zapota seed

Kaushik Nath*, Mehul Thummar, Mahesh Vaghela & Pranab Jani

 

Department of Chemical Engineering, G H Patel College of Engineering & Technology, Vallabh Vidyanagar 388 120, India

Email: nath_kaushik@hotmail.com

 

The potential of activated carbon prepared from the seeds of Manilkara zapota, an agricultural waste, was assessed for adsorptive dephenolation from aqueous solution. ZnCl2 was used as the activating agent. Batch adsorption experiments were conducted to study the effect of various physicochemical parameters such as initial concentration, dose of adsorbent, initial pH, and temperature. The percentage removal of phenol was found to increase with the decrease in initial concentration of phenol. Maximum removal efficiency of 96% was achieved with 25 mg/L of initial phenol concentration at pH 4.0 and temperature 30oC. Equilibrium modelling by linearized adsorption isotherms revealed that Freundlich isotherm could well represent the observed data for phenol adsorption on activated carbon as compared to Langmuir isotherm. Thermodynamic studies revealed that the sorption of phenol by activated carbon is an endothermic process, showing increase in sorption at higher temperature. Comparison of various kinetic models based on correlation coefficients revealed that the pseudo second order model, an indication of chemisorption mechanism, fits better the experimental data than the pseudo first order Lagergren model.

Keywords: Activated carbon, Adsorption, Phenol, Equilibrium, Kinetics

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 541-546

 

 

Sorption studies on cresol red modified Amberlite IR400 (Cl-) resin: Binary and selective separation of Hg2+ions

 

Amjad Mumtaz Khan*a, Shalini Gautamb & Sajad A Ganaib

 

aDepartment of Applied Chemistry, Z H College of Engineering & Technology

bDepartment of Chemistry, Aligarh Muslim University, Aligarh 202 002, India

Email: amjad.mt.khan@gmail.com

 

Strong acid anion exchange resin Amberlite IR400 (Cl-) has been modified by sorption of cresol red. Equibration studies such as concentration, pH, time and temperature of the reagent on the adsorption of dye have been studied. The maximum uptake of cresol red was found to be 2.20 mol per 0.3 103 g resin at pH 1.0. On the basis of Kd values in solvents having different polarity and dissociation constant, important binary separations of metal ions namely Pb2+, Bi3+, Mn2+, Zn2+, Ni2+, Sn4, Hg2+, Mg2+, Zr4+, Ca2+, Ba2+ and Sr2+ have been achieved. To check the selectivity and the reproducibility of the method, separation of different amounts of Hg2+ has been achieved in a synthetic mixture containing Fe3+(5.58 mg), Cu2+ (6.35 mg), Al3+ (2.69 mg) and Ni2+ (5.68 mg). The results show that the cresol red modified resin may be used for the removal of Hg2+ from industrial and domestic wastes. Results also indicate that the modified resin may also be suitable as a packing material in column chromatography for pre-concentration and recovery of some metal ions from industrial effluents and wastewater.

Keywords: Sorption, Cresol red, Amberlite IR400 (Cl-), Mercury separation, Anion exchange resin

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 547-554

 

 

Miscibility of poly(methylmethacrelate) and cellulose acetate butyrate blends in dimethyl formamide

 

M Selvakumar & D Krishna Bhat*

 

Department of Chemistry, National Institute of Technology Karnataka, Surathkal, Mangalore 575 025, India

Email:denthaje@gmail.com

The miscibility of poly(methylmethacrelate) (PMMA) and cellulose acetate butyrate (CAB) blends in dimethyl formamide (DMF) have been investigated by viscosity, density, refractive index and ultrasonic velocity studies. The polymer-solvent and blend-solvent interaction parameters and heat of mixing have been calculated using the viscosity, density and ultrasonic velocity data. The results indicated the existence of positive interactions in the blend polymer solutions and that they are miscible in dimethyl formamide in the entire composition range between 303-323 K. The study also revealed that variation in the temperature does not affect the miscibility of PMMA and CAB blends in DMF significantly. The presence of hydrogen bonding in the blends in the solid state has also been indicated by FTIR studies. SEM images also supported the miscibility of blends.

Keywords: Polymer solutions, Ultrasonic velocity, Viscosity, Interaction parameter, Polymer blends, Miscibility, PMMA, CAB, DMF

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 555-559

 

 

Effect of screw speed and plasticizer on the torque requirement in single screw extrusion of starch based plastics and their mechanical properties

 

Madhusweta Das

 

Department of Agricultural and Food Engineering, Indian Institute of Technology, Kharagpur 721 302, India

Email: madhu@agfe.iitkgp.ernet.in

 

Rod-shaped plastics have been prepared from blends of corn starch (69%) and plasticisers (31%), in the form of glycerol and water in different proportions (ranging from no glycerol to full glycerol) using a single screw extruder (compression ratio 1:1) (Length: Diameter ratio of 20:1) operated at specified constant temperatures, viz., 70, 110 and 110C at feed, mixing (plasticizing) and metering zones of the barrel, respectively. The screw speed was varied between 20 and 80 rpm. The torque generated during extrusion was recorded and then ultimate tensile strength (UTS) and elongation at break (EB) were measured after storing the extrudates for nine months at ambient condition. There was no significant difference in torque requirement for extrusion for use of zero to 9% glycerol content in the blends; however, for glycerol content greater than 9%, the developed torque gradually increased over the full range of rpm used. The range of torque was from 4.5 to 28 Nm, and for each blend it passed through a minimum at 60 rpm. Ultimate tensile strength followed a decreasing trend with increase in glycerol content in the plasticizer mix. However, elongation at break followed an increasing trend for increase in glycerol content up to 24%, and thereafter followed a decreasing trend with further increase in glycerol content. For glycerol content less than 24%, screw speed had a strong and direct bearing on UTS, but for EB, screw speed had no specific trend.

Keywords: Single screw extrusion, Plasticizer, Starch based plastics

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 560-565

 

 

Effect of Na3PO4 on the corrosion inhibition efficiency of EDTA Zn2+ system for carbon steel in aqueous solution

 

T Umamathi1, J Arockia Selvi2, S Agnesia Kanimozhi2, Susai Rajendran2* & A John Amalraj3

 

1Department of Chemistry, Kamaraj College of Engineering &Technology, Virudhunagar 626 001, India

2Corrosion Research Centre, Postgraduate and Research Department of Chemistry, GTN Arts College, Dindigul 624 005, India

3Department of Chemistry, Loyola College, Chennai 600 034, India

Email: srmjoany@sify.com

 

The synergistic effect of sodium salt of ethylene diamine tetra acetic acid (EDTA) and Zn2+in controlling corrosion of carbon steel in an aqueous environment containing 60 ppm Cl- was evaluated by weight loss and electro chemical methods. A formulation consisting of 50 ppm EDTA and 50 ppm Zn2+ was observed to achieve 73% inhibition efficiency. Influence of Na3PO4 on EDTA - Zn2+ was studied and IE was found to increase from 73 to 98%. A suitable mechanism of corrosion inhibition has been proposed which is based on the results obtained from weight loss method, polarization study, AC impedance spectra and FTIR spectra. The protective film consisted of Fe2+ - EDTA, Fe2+ - Na3PO4 complexes and Zn(OH)2.

Keywords: Carbon steel, Corrosion inhibition, EDTA, Na3PO4 , Synergistic effect

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 566-571

 

 

Catalytic conversion of propane to functionalized products

 

M Asadullah*, M Shamim Uddin, M Israt Jahan, M Abdul Motin & M Asaduzzaman

 

Department of Applied Chemistry and Chemical Technology, University of Rajshahi, Rajshahi 6205, Bangladesh

Email: asad@ru.ac.bd; asadullah8666@yahoo.com

 

Functionalization of propane has been investigated using various types of transition metal catalysts in the presence of K2S2O8 as oxidant in CF3COOH (TFA) solvent. In this reaction the primary products were iso-propyl alcohol and n-propyl alcohol. However, when the reaction was carried out in the TFA solvent, the primary products subsequently reacted with carboxylic acid, yielding iso-propyl trifluoroacetate as the dominant product and n-propyl trifluoroacetate as a minor product. Vanadyl acetylacetonate, VO(acac)2 ([CH3COCH=C(O-)CH3]2VO), exhibited the highest activity for propane functionalization. The optimum reaction conditions were 70C, 10 mmol of K2S2O8, 5 mL of trifluoroacetic acid, 0.1 mmol of catalyst and 0.75 mmol of propane in a 25 mL autoclave. The turn over number was found to be 15.5 and the yield was 1.61 mol% based on propane when 98 mmol (7 atm) of propane was used in a 200 mL autoclave. The yield was as high as 89 mol% when 0.75 mmol (1 atm) of propane was used in a 25 mL autoclave under optimum reaction conditions.

Keywords: Homogeneous catalysis, Propane, Functionalization, Potassium persulphate, Trifluoroacetic acid

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 572-575

 

 

Application of monolithic stirred reactor in hydrogenation reaction

 

Chintan K Joshi & Parimal A Parikh*

 

Chemical Engineering Department, S.V. National Institute of Technology, Surat 395 007, India

 

Hydrogenation of 1-octene was carried out in a monolithic stirred reactor. A cordierite monolith with HZSM-5 washcoat, impregnated with Pd was used as catalyst. The reactions were conducted between 50-65C and 250-400 psi hydrogen pressure. The effects of temperature and pressure on conversion and selectivity were measured. Major part of 1-octene was converted to n-octane and some isomers of octene were formed. However, at higher operating condition they were converted to n-octane. The experiments proved that the monolithic reactor is an attractive alternative reactor.

Keywords: Multiphase reactor, Pd/HZSM-5 monolith catalyst, Hydrogenation

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 576-580

 

 

Zinc-poly(aniline) rechargeable battery assembled with aqueous electrolyte

 

Bharat Chandra Dalui, I N Basumallick & Susanta Ghosh*

 

Department of Chemistry, Visva-Bharati, Santiniketan 731 235, India

Email: susanta.ghosh@visva-bharati.ac.in

 

The electrochemical behaviours of electro-synthesized poly(aniline) (PANI) cathode and commercial zinc anode in an aqueous electrolyte were investigated. The poly(aniline) sulphate cathodes were synthesized by galvanostatic oxidation of aniline from a sulphuric acid bath on platinum substrate and characterized by UV-Visible and FTIR spectroscopy. The electrolyte is comprised of ammonium sulphate and zinc sulphate dissolved in double distilled water. The slow scan linear voltammetry (Tafel plot) for the commercial zinc anode was recorded in this electrolyte and compared with its behaviour in chloride electrolyte. A negative shift of the open circuit potential of 55 mV, decrease in exchange current density of one order, and increase of cathodic Tafel slope in this sulphate electrolyte was observed. The above facts enhance the cell potential and the reversibility of the cell having the configuration, PANI || (NH4)2SO4, ZnSO4 (aq) || Zn. A discharge pleato with an average discharge potential of 1.1 V, which varied depending on the discharge current density, was observed for this reversible cell. The maximum discharge capacity, observed from this cell in the sulphate electrolyte, is 137 mAh.g-1.

Keywords: Poly(aniline), Ammonium sulphate, Zinc, Galvanostatic, Tafel plot

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 581-587

 

 

Development of Zn-Co alloy coatings by pulsed current from chloride bath

 

V Thangaraj, N K Udayashankar+ & A Chitharanjan Hegde*

 

Department of Chemistry, +Department of Physics, National Institute of Technology Karnataka,
Srinivasnagar 575 025, India

Email: achegde@rediffmail.com

 

Zinc-M (where M = Ni, Co and Fe) alloy is of great interest owing to their better mechanical and corrosion properties compared with pure zinc coatings. Corrosion resistance of Zn-Co alloy coatings can be improved considerably by pulse plating. The paper details the optimization of Zn-Co alloy bath using pulsed current and details the superiority of pulse plating over direct current plating. Electroplating of Zn-Co alloys over mild steel was carried out under different conditions of pulse parameters like duty cycle, frequency and peak current density. The production and properties of the deposits were found to be influenced by pulse parameters employed. Within the ranges studied, the bath follows anomalous codeposition with preferential deposition of less noble zinc. The influence of current density on %wt. of Co in the deposit and cathode current efficiencies was studied. It was observed that the deposit at average current density of 5.0 A.dm-2, 50% duty cycle and 100 Hz frequency showed excellent corrosion resistance with fine structure. The peak performance of pulse electrodeposit against corrosion was attributed to the change in the surface homogeneity as evidenced by scanning electron microscope (SEM) image. The drastic decrease of corrosion rate in pulse electrodeposit was attributed to the formation of semiconductor films on the surface as supported by impedance spectroscopy signals.

Keywords: Zn-Co alloy, Electrodeposition, Pulse plating, Corrosion, SEM

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 588-592

 

 

Bioleaching of low-grade copper ore using indigenous microorganisms

 

D Pradhan, S Pal, LB Sukla, G Roy Chaudhury & T Das*

 

Institute of Minerals and Materials Technology, Bhubaneswar 751 013, India

Email: truptidas@immt.res.in, trupti.sreyas@gmail.com

 

Investigations have been carried out on the iron oxidation rate of Acidithiobacillus ferrooxidans, the bacteria used in bioleaching process. It was observed that the regeneration time of the bacteria is reduced from 168 to 16 h by repeated sub-culturing, which in turn gave rise to higher iron oxidation rate thus increasing the kinetics of the process. This active strain was utilized for bioleaching of low-grade copper ore by varying two parameters namely, pH and pulp density. It was observed that around 30% of copper could be leached at an initial pH and pulp density of 2 and 20% (m/v) respectively.

Keywords: Bioleaching, Low-grade copper ore, Acidithiobacillis ferrooxidans

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 593-597

 

 

Voltammetric DNA biosensor for the study of mechanism of action of
anticancer drug-adriamycin

Sweety Tiwari & K S Pitre*

 

Department of Chemistry, Dr. Harisingh Gour Vishwavidyalaya, Sagar 470 003, India

Email: kspitre@rediffmail.com

 

The interaction of anticancer drug adriamycin with DNA has been studied using voltammetric DNA biosensor. The binding mechanism of adriamycin was elucidated by using differential pulse voltammetry (DPV) at DNA modified glassy carbon fiber electrodes (GCFE). The decrease in guanine oxidation peak current at +0.9 V was used as an indicator for interaction mechanism in acetate buffer (pH 4.5). The studied drug-DNA interaction mechanism at charged electrode surface is similar to the in-vivo DNA-drug complex formation, where DNA is in close contact with charged phospholipid membranes and proteins. Thus, the fabricated biosensor helps in understanding the in-vivo mechanism of action of this anticancer drug.

Keywords: Modified electrode, Biosensor, Adriamycin, Anthracycline compound

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 598-603

 

 

Stability-indicating HPLC method for determination of vitamins B1, B2, B3 and B6 in pharmaceutical liquid dosage form

 

Shino Thomasa, Rakesh Kumarb, Ashutosh Sharmab, Roshan Issarania & Badri Prakash Nagoria*

 

aPharmacy Wing, Lachoo Memorial College of Science and Technology, Jodhpur 324 003, India

bDepartment of Analytical Research, Ranbaxy Research Lab., Gurgaon 122 015, India

Email: bpnagori@sancharnet.in

 

A simple, selective, precise and stability-indicating high-performance liquid chromatographic (HPLC) method for determination of vitamins B1, B2, B3 and B6 in pharmaceutical liquid dosage form was developed and validated. The chromatographic conditions comprised a reversed-phase C18 column (250 4.6 mm), 5 with a mobile phase consisting of a mixture of solution (0.015 M 1-hexane sulphonic acid sodium salt, pH 3.0 0.05) and methanol in gradient elution. The flow rate was kept at 1.5 mL/min. and the detection was carried out at 280 nm. The retention times of vitamins B3, B2, B6 and B1 were 6.3, 15.1, 19.9 and 42.7 min. respectively. The linear regression analysis data for the calibration plots showed good linear relationship with coefficient of correlation values, r = 0.999 for vitamins B1, B2, B3 and B6 in the concentration ranges of 23.08-42.85, 9.61-17.84, 116.36-216.09 and 7.01-13.02 g/mL respectively. The method was validated for precision, recovery and robustness. The vitamins undergo degradation under acidic, basic, peroxide, photochemical and thermal conditions. Statistical analysis proves that the method is reproducible and selective for the estimation of vitamins under study. As the method could effectively estimate the vitamins in presence of their degradation products, it can be employed as a stability-indicating method.

Keywords: Vitamin B1, B2, B3 and B6, HPLC, Stability indicating, Validation

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 604-612

 

 

Submerged membrane bioreactor system for municipal wastewater treatment process: An overview

 

Neha Gupta, N Jana & C B Majumder*

 

Department of Chemical Engineering, Indian Institute of Technology, Roorkee 247667, India

Email: chandfch@iitr.ernet.in

 

The submerged membrane bioreactor (SMBR) is a promising technology for wastewater treatment and water reclamation. This paper provides an overview of wastewater treatment in a submerged membrane bioreactor process with a special focus on municipal wastewater systems. SMBRs predict more than 95% organic removal with relative short hydraulic retention times (HRTs) of 1-8 h and NH3 removal of more than 90% in the municipal wastewater treatment. It achieves 30% more removal of organic matter than activated sludge process. The COD can be reduced by 95%. Nitrification was complete and up to 82% of the total nitrogen could be denitrified. Nitrification/ denitrification is sensitive to the feed quality, such as dissolved oxygen (DO) concentration, temperature, organic loads, inorganic/ organic compounds and pH. The feed water composition, membrane geometry/configuration, membrane materials, and hydrodynamic effects are responsible for membrane fouling. These drawbacks can be reduced by maintaining turbulent conditions, operating at critical flux, and by selection of a suitable fouling resistance membrane material. Membrane washing is performed when the permeability is less than 10% of initial permeability. The reactor performance and the stability of the process and the membrane capacity are also discussed. Details of the various methods for washing are also included.

Keywords: Submerged membrane, Bioreactor, Wastewater treatment, Nirification, Hydrarulic retention time

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 613-616

 

 

Synthesis, characterization and electrical conductivity of charge transfer complex of
p-toluidine and chloranil

 

 

Rakesh Sharma, Mukesh Paliwal1, Sadhana Singh, Rameshwer Ameta2 & Suresh C Ameta1*

 

Department of Chemistry, B N P G College, Udaipur 313 001, India

1Department of Chemistry, M L Sukhadia University, Udaipur 313 001, India

2Government Meera Girls College, Udaipur 313 001, India

Email: ameta_sc@yahoo.com

 

The charge transfer (CT) complex of p-toluidine chloranil (CA) was prepared by solution growth and microwave techniques and characterized by elemental analysis, UV-Vis, IR and 1H NMR spectroscopic data. The data obtained indicated the formation of the CT complex having 1:1 stoichiometry. It indicates that the charge transfer interaction is associated with a proton migration from the acceptor to the donor followed by intermolecular hydrogen bonding. The CT complex was found to behave as semiconductor at room temperature.

Keywords: CT complex, Microwave method, p-Toluidine, Chloranil (CA)

 

 

Indian Journal of Chemical Technology

Vol. 15, November 2008, pp. 617-620

 

 

Validated liquid chromatographic method for simultaneous estimation of albendazole and ivermectin in tablet dosage form

 

Anil Waldiaa, Shubash Guptab, Roshan Issarania & Badri P Nagoria*

 

aPharmacy Wing, Lachoo Memorial College of Science and Technology, Jodhpur, 342 003, India

bOasis Test House Ltd., 22 Godam Industrial Area, Jaipur, India

Email: bpnagori@sancharnet.in

 

A reverse phased liquid chromatography (LC) method was developed and validated for simultaneous estimation of albendazole and ivermectin in tablet dosage form. The isocratic LC analysis was performed on NUCLEODUR C18 RP column (250 x 4.6 mm, 5 ) using mobile phase composed of acetonitrile, methanol and water in ratio of 60:30:10 (v/v/v) at a flow rate of 1.8 mL/min. Quantitation was performed using UV detector at 245 nm and the run time was 20 min. The retention times were found to be 3.56 min for albendazole and 10.08 min for ivermectin. The analytical method was validated according to ICH guidelines. The linearity was observed in the range of 400800 and 612 mg/mL with correlation coefficient, r=0.9975 and 0.9969 for albendazole and ivermectin respectively. The relative standard deviation values for repeatability and intermediate precision studies were less than 2%, and the accuracy (% recovery) was greater than 98% for both the drugs. The method was successfully applied for market sample analysis and mean percentage assay values were 98.680.28 and 98.670.50 for albendazole and ivermectin respectively. The present method is precise and accurate and can be used for the routine estimation of albendazole and ivermectin in tablet dosage forms.

Keywords: RP-HPLC, Albendazole, Ivermectin, Validation