Indian Journal of Experimental Biology

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VOLUME 49

NUMBER 8

AUGUST 2011

 

 

 

CODEN: IJEB (A6) 49 (8) - (2011)

ISSN: 0019-5189 (print) 0975-1009 (online)

CONTENTS

 

Review Article

 

 

 

Ethno biological usage of zoo products in rheumatoid arthritis

565

Antony Gomes, Md. Aftab Alam, Sourav Bhattacharya, Subir Chandra Dasgupta, Sanghamitra Mukherjee, Shamik Bhattacharya & Aparna Gomes

 

 

 

Papers

 

 

 

Inhibition of subcutaneous growth of Ehrlich ascites carcinoma (EAC) tumor by post-immunization with EAC-cell gangliosides and its anti-idiotype antibody in relation to tumor angiogenesis, apoptosis, cell cycle and infiltration of CD4+, CD8+ lymphocytes, NK cells, suppressor cells and APC-cells in tumor

574



Bipasha Mondal & Sandip Saha

 

 

 

Effect of b-arrestin 2 on cytokine production of CD4+ T lymphocytes of mice with allergic asthma

585

Guyi Wang, Yi Liu, Muyi Yang, Shaokun Liu, Libing Ma, Subo Gong,
Keng Li, Li Zhang & Xudong Xiang

 

 

 

Expression of rabies virus glycoprotein gene into eukaryotic system and determination of potential T-cell epitopes

594

Neha R Tomar, Rajesh Chandra, Rajiv Kumar, A K Tiwari & Anil Kumar

 

 

 

Sensitivity of last larval stadium of Chrysomya megacephala (Fabricius) to anti-allatin ageratochromene Precocene II

600

Satya Singh & Krishna Kumar

 

 

 

Possible nitric oxide mechanism in the protective effect of hesperidin against ischemic reperfusion cerebral injury in rats

609

Vaibhav Gaur, Aditi Aggarwal & Anil Kumar

 

 

 

Anti-depressant-like activity of a novel serotonin type-3 (5-HT3) receptor antagonist in rodent models of depression

619

Deepali Gupta, Thangaraj Devadoss, Shvetank Bhatt, Baldev Gautam,
Ankur Jindal, Dilip Pandey & Radhakrishnan Mahesh

 

 

Contd.

Evaluation of antioxidant and neuroprotective effect of date palm (Phoenix dactylifera L.) against bilateral common carotid artery occlusion in rats

627

Rohini R Pujari, Neeraj S Vyawahare & Virendra G Kagathara

 

 

 

Effect of exposure to extremely low electro-magnetic field during prenatal period on mice spleen

634

Parvin Dokht Bayat, Ali Ghanbari, Saeid Babaei, Mozafar Khazaei, Rostam Ghorbani & Mahmood Ayubian

 

 

 

Announcement

 

 

 

12th International Congress of Ethnopharmacology

564

 

 

Information for Authors

639

 

 

覧覧覧覧覧覧

 
Announcement

 

 

12th International Congress of Ethnopharmacology

 

17-19 February 2012, Kolkata, India

 

Organised by the School of Natural Product Studies, Jadavpur University, Kolkata, India, the theme of the Congress will be 典raditional Medicines and Globalization裕he Future of Ancient Systems of Medicine. The conference will focus on some crucial and contemporary issues on the scientific study, development and evaluation of natural products and will center on the theme of globalization of ancient systems of medicines. Topics to be covered in the conference are:
(i)
Ethno pharmacological perspectives裕raditional to modern pharmaceuticals, (ii) Traditional medicine inspired drug discovery and development, (iii) Global trade in and commercialization of Ayurveda and other ancient system of medicines, (iv) Interface between history and ethnopharmacology of traditional medicines, (v) Trends in the evaluation of traditional medicine, (vi) Promotion and development of natural products with international cooperation,
(vii) Polypharmacology of herbal medicine, (viii) Traditional medicine and neglected diseases, (ix) Natural Products from marine sources, (x) Traditional medicines and the world wide web: Globalising local knowledge or localizing global technologies, (xi) System biology and synergy in natural products, (xii) Evaluation of traditional medicines容vidence-based health care claims, (xiii) The future of traditional medicines
multidisciplinary approaches in health sciences,
(xiv) Ethno veterinary medicine, (xv) Phytochemical, pharmacological and clinical studies on natural products, (xvi) Traditional medicines and biomedicine幼ommon or conflicting interests? and (xvii) Ethnopharmacology and bio-cultural diversity. For further details please contact the Organizing Secretary,
ISE 2012, Prof. Pulok K. Mukherjee, Director, School of Natural Product Studies, Jadavpur University, Kolkata 700 032, India. Telefax: + 91 33 24146046.
E-mail: naturalproductm@gmail.com. Website: www.ise-snpsju.org

 

覧覧覧覧覧

 

Author Index

Aggarwal Aditi

609

Alam Md. Aftab

565

Ayubian Mahmood

634

 

 

Babaei Saeid

634

Bayat Parvin Dokht

634

Bhatt Shvetank

619

Bhattacharya Shamik

565

Bhattacharya Sourav

565

 

 

Chandra Rajesh

594

 

 

Dasgupta Subir Chandra

565

Devadoss Thangaraj

619

 

 

Gaur Vaibhav

609

Gautam Baldev

619

Ghanbari Ali

634

Ghorbani Rostam

634

Gomes Antony

565

Gomes Aparna

565

Gong Subo

585

Gupta Deepali

619

 

 

Jindal Ankur

619

 

 

Kagathara Virendra G

627

Khazaei Mozafar

634

Kumar Anil

594

Kumar Anil

609

Kumar Krishna

600

Kumar Rajiv

594

 

 

Li Keng

585

Liu Shaokun

585

Liu Yi

585

 

 

Ma Libing

585

Mahesh Radhakrishnan

619

Mondal Bipasha

574

Mukherjee Sanghamitra

565

Pandey Dilip

619

Pujari Rohini R

627

 

 

Saha Sandip

574

Singh Satya

600

 

 

Tiwari A K

594

Tomar Neha R

594

 

 

Vyawahare Neeraj S

627

 

 

Wang Guyi

585

 

 

Xiang Xudong

585

 

 

Yang Muyi

585

 

 

Zhang Li

585

 

 

Keyword Index

b 2 adrenergic receptors

585

Alternative medicine

565

Angiogenesis

574

Anti-allatin

600

Anti-depressant-like effect

619

Anti-idiotype

574

Apoptosis

574

b -arrestin 2

585

Asthma

585

 

 

Bilateral common carotid artery occlusion

609

Bilateral common cerebral artery occlusion

627

 

 

CD4 + T lymphocytes

585

Chrysomya megacephala

600

 

 

Depression

619

DNA vaccine

594

 

 

Electromagnetic field

634

Epitope mapping

594

Flavonoids

627

Forced swim test

619

Fourth generation insecticide

600

 

 

Gangliosides

574

Glycoprotein

594

 

 

Hesperidin

609

5-HT 3 antagonist

619

 

 

Indirect fluorescent antibody test

594

Interleukin-4

585

Ischemia

609

 

 

Juvenile hormone

600

 

 

Megakaryocyte

634

Mice

634

 

 

Oxidative stress

609

Oxidative stress

627

Phoenix dactylifera

627

Precocene II

600

Precocious metamorphosis

600

Pupal-adult mosaics

600

 

 

Quinoxalin-2-carboxamide

619

 

 

Rabies virus

594

Respiratory enzyme activity

609

Rheumatoid arthritis

565

 

 

Serotonin

619

Spleen

634

Stereology

634

Stroke

609

 

 

Traditional medicine

565

Tumor

574

 

 

Zoo products

565

 

 

 

Correspondent author has been indicated by * sign

 

 

Indian Journal of Experimental Biology

Vol. 49, August 2011, pp. 565-573

 

 

Review Article

 

 

Ethno biological usage of zoo products in rheumatoid arthritis

Antony Gomes , Md. Aftab Alam & Sourav Bhattacharya

Laboratory of Toxinology and Experimental Pharmacodynamics

Department of Physiology, University of Calcutta

92, Acharya Prafulla Chandra Road

Kolkata 700 009, India

Subir Chandra Dasgupta & Sanghamitra Mukherjee

Postgraduate Department of Zoology

Maulana Azad College

8, Rafi Ahmed Kidwai Road, Kolkata 700 013, India

Shamik Bhattacharya & Aparna Gomes*

Drug Development Diagnostics and Biotechnology Division

Indian Institute of Chemical Biology (IICB), CSIR

4, Raja S C Mullick Road , Kolkata 700 032, India

Rheumatoid arthritis (RA) is one of the most common autoimmune disorder which causes swelling, redness, pain, stiffness, restriction of limb movements, decreases life expectancy and early death of the patients. Available drugs include non steroidal anti-inflammatory and analgesics, disease modifying anti-rheumatic drugs and steroids (glucocorticoids etc). All these drugs have their own limitations such as gastrointestinal irritations, cardiovascular problems, and drug dependency. Search for alternative therapy from natural products are being ventured throughout the world. Zoo therapy in arthritis, a common practice of the ancient times that have been mentioned in traditional and folk medicine. The scientific basis of some of the zoo products are being explored and have been showing promising results in experimental rheumatoid arthritis. These therapies have minimum side effects and many of them have potential to give rise to drug development clues against rheumatoid arthritis. The present review is an effort to establish the folk and traditional treatment of rheumatoid arthritis using zoo products.

 

 

Indian Journal of Experimental Biology

Vol. 49, August 2011, pp. 574-584

 

 

Papers

 

 

Inhibition of subcutaneous growth of Ehrlich ascites carcinoma (EAC) tumor by post吠mmunization with EAC-cell gangliosides and its anti-idiotype antibody in relation to tumor angiogenesis, apoptosis, cell cycle and infiltration of CD4+, CD8+ lymphocytes, NK cells, suppressor cells and APC-cells in tumor

Bipasha Mondal & Sandip Saha*

Department of Metabolic Regulation, Chittaranjan National Cancer Institute (CNCI)
37, S. P. Mukherjee Road , Kolkata 700 026, India

Received 9 August 2010; revised 6 May 2011

Both EAC-tumor associated gangliosides and its anti-idiotype antibody inhibited growth of this tumor significantly. Immuno-histological studies with von Willebrand Factor (vWF) antibody indicated that tumor angiogenesis as determined by expression of vWF decreased in tumors of mice, post-immunized with EAC-cell gangliosides as well as its anti-idiotype antibody. Infiltration of various immune cells of the host in the tumor correlated to some extent with tumor-growth inhibition. Apoptosis study using AnnexinV-FITC and propidium iodide indicated that tumor growth inhibition in mice post-immunized with EAC-gangliosides and its anti-idiotype antibody were due to enhanced apoptosis and cell death. Cell cycle analysis by FACS indicated that EAC-cell associated gangliosides and its anti-idiotype antibody were acting both at the M2 i.e. S and M3 i.e. G2/M phases of the cell cycle to arrest tumor growth.

 

 

Indian Journal of Experimental Biology

Vol. 49, August 2011, pp. 585-593

 

 

Effects of ゚-arrestin 2 on cytokine production of CD4 + T lymphocytes of mice with allergic asthma

Guyi Wang 1 , Yi Liu 1 , Muyi Yang 2 , Shaokun Liu 1 , Libing Ma 1 , Subo Gong 1 , Keng Li 1 , Li Zhang 1 & Xudong Xiang 1 *

1 Department of Respiratory Medicine , the Second Xiangya Hospital of Central South University , Changsha, Hunan 410011, China

2 The Changsha Central Hospital , Changsha , Hunan 410004 , China

Received 25 October 2010; revised 18 May 2011

゚-arrestin 2 has been shown to participate in the pathogenesis of asthma by inducing Th2 cell migration to the lungs. Whether ゚-arrestin 2 regulates cytokine production of CD4 + T cells is still unknown. The aim of the present study was to investigate the effect of ゚-arrestin 2 on the cytokine production of CD4 + T lymphocytes and the mechanism involved in a mouse model for asthma. After silencing ゚-arrestin 2 expression in CD4 + T lymphocytes from asthmatic mice by RNA interference (RNAi), the interleukin-4 (IL-4) and interferon- ? (IFN-?) levels in CD4 + T lymphocyte culture supernatants with or without terbutaline stimulation were determined. Cell-surface ゚2 adrenergic receptor ( b 2AR) as well as GATA3 expression of CD4 + T lymphocytes were also measured. CD4 + T lymphocytes of mice with allergic asthma expressed higher levels of ゚-arrestin 2 on both mRNA and protein levels. ゚-arrestin 2 RNAi decreased IL-4 (43.16%) and GATA3 (protein 77.21%, mRNA 62.98%) expression after terbutaline stimulation. Cell-surface ゚2AR of CD4 + T lymphocytes decreased (15.27%) after terbutaline treatment, but recovered after ゚-arrestin 2 RNAi down-modulation. These findings demonstrate that ゚-arrestin 2 regulates IL-4 production and GATA3 expression of CD4 + T lymphocytes partly through the ゚2AR signaling pathway in an allergic asthma model.

 

 

Indian Journal of Experimental Biology

Vol. 49, August 2011, pp. 594-599

 

 

Expression of rabies virus glycoprotein gene into eukaryotic system
and determination of potential T-cell epitopes

Neha R Tomar 1* , Rajesh Chandra 2 , Rajiv kumar 3 , A K Tiwari 4 & Anil Kumar 1

1 Department of Molecular Biology and Genetic Engineering,

College of Basic Sciences and Humanities, 2 Department of Veterinary

Microbiology and Immunology, College of Veterinary and Animal Sciences,

G.B Pant University of Agriculture and Technology, Pantnagar 263145, India .

3 Animal Biotechnology Section, Central Sheep and Wool Research Institute, Avikanagar 304501, India .

4 Molecular Biology Laboratory, Division of Animal Biotechnology, Indian Veterinary Research Institute,

Izatnagar 243122, India .

Received 24 December 2010; revised 18 April 2011

The present study was undertaken to clone, express rabies virus glycoprotein (RVG) and to identify potential T-cell epitopes on it. RVG gene (1590 bp) was amplified using gene specific primers. The amplified product was cloned into pTZ57R/T cloning vector by TA cloning. RVG gene was subcloned into pcDNA3.1 (+) expression vector. In this study, cloning and expression of rabies virus glycoprotein gene was done under CMV promoter and an expression construct (pcDNA.RVG) was prepared and clones were confirmed by restriction digestion, colony PCR and nucleotide sequencing. The expression construct was further characterized by western blotting and indirect fluorescent antibody test (IFAT).
In silico
analysis of this protein was done to find out potential antigenic sites so that it can be further evaluated for its potential as candidate for epitope vaccine against rabies.

 

 

Indian Journal of Experimental Biology

Vol. 49, August 2011, pp. 600-608

 

 

Sensitivity of last larval stadium of Chrysomya megacephala (Fabricius) to anti-allatin ageratochromene Precocene II

Satya Singh & Krishna Kumar*

Department of Zoology, University of Allahabad , Allahabad 211 002, India

Received 8 November 2010; revised 19 April 2011

Treatment of early and late third instar larvae of economically important insect C. megacephala with the ageratochromene precocene II resulted into precocious metamorphosis. The other effects included mortality, production of pupal-adult mosaics, reduction in percentage of adult emergence and formation of non-viable deformed adults that died after a few hours of emergence. The application of precocene II adversely hampered the normal growth, development and metamorphosis of the insect and the results show that precocene II has vast potential for excellent control of this insect of medico-veterinary importance and can be used in insect pest management programs.

 

 

Indian Journal of Experimental Biology

Vol. 49, August 2011, pp. 609-618

 

 

Possible nitric oxide mechanism in the protective effect of hesperidin against ischemic reperfusion cerebral injury in rats

Vaibhav Gaur, Aditi Aggarwal & Anil Kumar*

Pharmacology d ivision, University Institute of Pharmaceutical Sciences,

Panjab University , c handigarh 160014, India

Received 16 October 2010; revised 2 May 2011

Stroke is the third leading cause of death and disability around the globe. The aim of the present investigation was to evaluate the protective effect of hesperidin and its nitric oxide mechanism against cerebral ischemia reperfusion injury. Bilateral common carotid artery occlusion for 30 min followed by 24 h reperfusion was given to induce ischemia in rats. Animals were pretreated with hesperidin (50 and 100 mg/kg, po) for 7 days. Various behavioural tests, oxidative stress parameters, endogenous antioxidant system, antioxidant enzyme activity and mitochondrial enzyme complex (I, II, III and IV) dysfunctions in cortex and striatum were assessed subsequently. Hesperidin (50 and 100 mg/kg) significantly improved neurobehavioral alterations (neurological score, locomotor activity, resistance to lateral push and hanging wire latency), attenuated oxidative damage, restored antioxidant and mitochondrial complex enzyme activities in cortex and in striatum regions of the brain as compared to their respective controls. L-arginine (100 mg/kg) or L-NAME (10 mg/kg) pretreatment with lower dose of hesperidin (50 mg/kg) significantly reversed or potentiated its protective effect, respectively which was significant as compared to hesperidin (50 mg/kg). The results highlight the involvement of nitric oxide mechanism in the protective effect of hesperidin against ischemia reperfusion injury induced alterations.

 

 

Indian Journal of Experimental Biology

Vol. 49, August 2011, pp. 619-626

 

 

Anti-depressant-like activity of a novel serotonin type-3 (5-HT 3 ) receptor antagonist in rodent models of depression

Deepali Gupta*, Thangaraj Devadoss, Shvetank Bhatt, Baldev Gautam, Ankur Jindal, Dilip Pandey &
Radhakrishnan Mahesh

Department of Pharmacy, FD-III, Birla Institute of Technology & Science (BITS), Pilani, 333 031, India .

Received 25 October 2010; revised 6 May 2011

N -Cyclohexyl-3-methoxyquinoxalin-2-carboxamide (QCM-13) , a novel 5-HT 3 antagonist identified from a series of compounds with higher pA 2 (7.6) and good log P (2.91) value was screened in rodent models of depression such as forced swim test (FST), tail suspension test (TST), interaction studies with standard anti-depressants and confirmatory studies such as reversal of parthenolide induced depression and reserpine induced hypothermia. In FST (2 and 4 mg/kg) and TST (2 and 4 mg/kg), QCM-13 significantly reduced the duration of immobility in mice without affecting the base line locomotion. QCM-13 (2 and 4 mg/kg) was also found to have significant interaction with standard anti-depressants (fluoxetine and bupropion in FST and TST respectively). Further, reversal of parthenolide induced depression in mice and reserpine induced hypothermia in rat models indicate the serotonergic influence of QCM-13 for anti-depressant potential.

 

 

Indian Journal of Experimental Biology

Vol. 49, August 2011, pp. 627-633

 

 

Evaluation of antioxidant and neuroprotective effect of date palm
( Phoenix dactylifera L.) against bilateral common carotid
artery occlusion in rats

Rohini R Pujari 1, *, Neeraj S Vyawahare 2 & Virendra G Kagathara 3

Department of Pharmacology, AISSMS College of Pharmacy , Kennedy Road , Near RTO, Pune-411 001, India

Received 9 July 2010; revised 13 May 2011

The cerebral ischemia in rats was induced by occluding bilateral common carotid arteries (BCCAO) for 30 min., followed by 45 min reperfusion. BCCAO caused significant depletion in superoxide dismutase, catalase, glutathione, glutathione peroxidase, glutathione-S-transferase, glutathione reductase and significant increase in lipid peroxidation along with severe neuronal damage in the brain. All the alterations except depletion in glutathione peroxidase and glutathione-S-transferase levels induced by cerebral ischemia were significantly attenuated by 15 days pretreatment with methanolic extract of P. dactylifera fruits (100, 300 mg/kg), whereas 30 mg/kg dose was insignificant in this regard. These results suggest the possible use P. dactylifera against bilateral common carotid artery occlusion induced oxidative stress and neuronal damage.

 

 

Indian Journal of Experimental Biology

Vol. 49, August 2011, pp. 634-638

 

 

Effect of exposure to extremely low electro-magnetic field during
prenatal period on mice spleen

Parvin Dokht Bayat 1 , Ali Ghanbari 2 *, Saeid Babaei 1 , Mozafar Khazaei 2 , Rostam Ghorbani 2 & Mahmood Ayubian 3

1 Department of Anatomy, Arak University of Medical Sciences, Arak , Iran

2 Fertility and Infertility Research Center , Kermanshah University of Medical Sciences,
P.O. Box 1568 , Kermanshah , Iran

3 Department of Anatomy, Tehran University of Medical Sciences, Tehran , Iran

Received 8 November 2010; revised 5 May 2011

Total body weight of newborns, the volume of spleen, and the number of megakaryocytes decreased following the exposure to ELF-MF (6x10 -3 T and 50 Hz) at 1-5, 6-10, 11-15, and 16-20 days of pregnancy of mice. The complete period of gestation was sensitive to ELF-MF exposure; the initial days were more prone to exposure. The results suggest that the use of ELF-MF producing instruments should be limited during gestation.