Indian Journal of Experimental Biology

http://www.niscair.res.in; http://nopr.niscair.res.in

 

Total visitors: 4502 since 03-04-2012

 

 

VOLUME 50

NUMBER 4

APRIL 2012

CODEN: IJEB (A6) 50 (4) 243-308 (2012)

ISSN: 0019-5189 (Print); 0975-1009 (Online)

 

CONTENTS

 

 

Papers

 

Quinazolinone exposure induces apoptosis and changes expressions of Fas/FasL and C-Flip in embryonic mice testicles

247

Maryam Shams Lahijani, Shirin Farivar, Mojgan Sarhady & Mitra Amiri

 

 

 

Purification of recombinant antigen BmSXP used in panLF Rapid kit for lymphatic filariasis

256

Teng Kew Khoo, Rahmah Noordin & Amutha Santhanam

 

 

 

Mosquito cell line C6/36 shows resistance to Cyt1Aa6

265

Lingling Zhang, Enjiong Huang, Baozhen Tang , Xiong Guan & Ivan Gelbič

 

 

 

Immunomodulatory effects of alcoholic and hydroalcoholic extracts of Moringa olifera Lam leaves

270

Otilia J F Banji, David Banji & Kavitha R

 

 

 

Wound healing activity of ethanolic extract of Shorea robusta Gaertn. f. resin

277

T A Wani, H H Chandrashekara, D Kumar, R Prasad, A Gopal, K K Sardar, S K Tandan & D Kumar

 

 

 

Rapid screening and cultivation of oleaginous microorganisms

282

Xinlei Gao, Ye Liu Zhongju Chen & Li Wu

 

 

 

Decolourization and degradation of C.I.Reactive Red 195 by Georgenia sp.CC-NMPT-T3

290

Madhuri Sahasrabudhe & Girish Pathade

 

 

 

Cadmium tolerance and antibiotic resistance in Escherichia coli isolated from waste stabilization ponds

300

Sova Patra, T K Das, C Avila, V Cabello, F Castillo, D Sarkar (Paria), Susmita Lahiri (Ganguly) & B B Jana

 

 

覧覧覧覧覧覧

NISCAIR痴 Policy on Plagiarism

The system of formal communication in science through publication in primary journals is based on originality and quality of information being the only criteria for publication. However, there have been tendencies to misuse the system and vitiate the process of science communication for personal benefits. One of the ills afflicting science communication is plagiarism. Attempts at plagiarism may range from verbatim, copying of extensive material of other authors, misappropriating results/data of others with minor changes in language/presentation without giving credit to original source, to publish essentially the same information more than once.

As the premier publisher in India of primary scientific journals in various disciplines of science and technology, NISCAIR strongly reiterates its policy of discouraging plagiarism of all kinds. All efforts are made detect and frustrate attempts at plagiarism through editorial screening and rigorous peer review in respect of communications received for publication in NISCAIR publications. Cooperation of the scientific community is sought in our efforts to frustrate all attempts at plagiarism.

In case any attempt to plagiarize is brought to our attention accompanied with convincing evidence, following steps would be taken:

(a)                            After consulting the respective Editorial Board Members, authors guilty of plagiarism will be debarred from publishing their papers in NISCAIR journals

(b)                           Heads of the departments/institutes of the offending authors will be intimated of such incidences of plagiarism.

(c)                            Such incidents of plagiarism will be publicized through the concerned NISCAIR journals in consultation with the respective Editorial Board Members.

 

覧覧覧覧覧覧覧

 

Editor痴 Note

 

The Indian Journal of Experimental Biology is covered by the following international abstracting and indexing services:

 

Science Citation Index ExpandedTM

PubMed (http://www.ncbi.nim.nih.gov/)

MEDLINE

BIOSIS

Chemical Abstracts Service

Excerpta Medica

Informascience

Refrativnyi Zhurnal

Zoological Records

覧覧覧覧覧覧覧

 

 

Author Index

Amiri Mitra

247

Avila C

300

 

 

Banji David

270

Banji Otilia J F

270

 

 

Cabello V

300

Castillo F

300

Chandrashekara H H

277

Chen Zhongju

282

 

 

Das T K

300

 

 

Farivar Shirin

247

 

 

Gao Xinlei

282

Gelbič Ivan

265

Gopal A

277

Guan Xiong

265

Huang Enjiong

265

 

 

Jana B B

300

 

 

Khoo Teng Kew

256

Kumar D

277

Kumar Dinesh

277

 

 

Lahiri (Ganguly) Susmita

300

Liu Ye

282

 

 

Noordin Rahmah

256

 

 

Pathade Girish

290

Patra Sova

300

Prasad R

277

R Kavitha

270

 

 

Sahasrabudhe Madhuri

290

Santhanam Amutha

256

Sardar K K

277

Sarhady Mojgan

247

Sarkar (Paria) D

300

Shams Lahijani Maryam

247

 

 

Tandan S K

277

Tang Baozhen

265

 

 

Wani T A

277

Wu Li

282

 

 

Zhang Lingling

265

 

 

 

Keyword Index

2-2 Azinobis-(3-ethylbenthiazoline 6 sulphonate)



290

Antibiotic resistance

300

Apoptosis

247

 

 

Bacillus thuringiensis

265

BmSXP recombinant antigen


256

 

 

C6/36 cells

265

Cadmium tolerance

300

C-Flip

247

 

 

Embryonic testis

247

Escherichia coli

300

Excised wound

277

 

 

Fas/FasL

247

Fatty acid composition

282

 

 

Humoral response

270

Hydroxyproline

277

 

 

Immunomodualtor

270

Incised wound

277

Indirect immuno fluorescence assay


265

 

 

Lignin peroxidase

290

Lymphatic filariasis

256

 

 

Moringa olifera

270

Mortierella isabellina CGMCC 3.3410O


282

 

 

Nutrient

270

 

 

Oil content

282

Oleaginous strain

282

 

 

PanLF Rapid

256

Phagocytic activity

270

Purification optimizations

256

 

 

Quinazolinones

247

 

 

Rapid determination method


282

Reactive Red 195

290

Receptor binding

265

Resistance

265

 

 

Shorea robusta

277

Synthetic medium

290

 

 

Waste stabilization pond

300

 

 

 

Correspondent author has been indicated by * sign

 

Papers

Indian Journal of Experimental Biology

Vol. 50, April 2012, pp. 247-255

 

 

Quinazolinone exposure induces apoptosis and changes expressions of
Fas/FasL and C-Flip in embryonic mice testicles

 

Maryam Shams Lahijania,*, Shirin Farivara, Mojgan Sarhadya & Mitra Amiria

Developmental Biology, Animal Sciences, Faculty of Biological Sciences, Shahid-Beheshti University (SBU),
G.C. ,Velenjak Tehran, Iran

Received 28 September 2011; revised 2 February 2012

Quinazolinones represent a class of sedative and anticancer drugs. Quinazolinones-based compounds have ability to suppress prostate tumor growth via apoptosis. Apoptosis is very common in embryos and adults of normal and injured mammalian testes. Effects a new derivative of quinazolinone (4(3H) quinazolinone-2-ethyl-2-phenyl ethyl (QEPE)), on the testis of Balb/C mice embryos were investigated. QEPE was able to reduce number of germ cells and diameter of seminiferous tubules. TUNEL assay analysis indicated that reduction correlated with an increase in the number of apoptotic cell. Furthermore, electron microscope observations confirmed typical apoptotic morphologies characterized by chromatin fragmentation. Finally, RTPCR analysis showed QEPE increases the levels of Fas/Fasl and decreases C-Flip mRNAs in the testis of exposed embryos.

 

 

Indian Journal of Experimental Biology

Vol. 50, April 2012, pp. 256264

 

 

Purification of recombinant antigen BmSXP used in panLF Rapid kit for lymphatic filariasis

Teng Kew Khoo, Rahmah Noordin & Amutha Santhanam*

Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), 11800, Pulau Pinang, Malaysia

Received 20 May 2011; revised 30 January 2012

A rapid antibody detection test is very useful for the detection of lymphatic filariasis, especially for certification and surveillance of post-mass drug administration. panLF Rapid kit is suitable for this purpose since it can detect all species of lymphatic filaria. It is based on the detection of anti-filarial IgG4 antibodies that react with recombinant B. malayi antigens, BmR1 and BmSXP. There is an increase demand for the test due to its attributes of being rapid, sensitive and specific results, as well as its field-applicability. The main aim of this paper is to obtain high recovery and purity of recombinant antigen BmSXP via a modified method of immobilized metal affinity chromatography (IMAC). The highest product yield of 11.82 mg/g dry cell weight (DCW) was obtained when IMAC was performed using the optimized protocol of 10 mM imidazole concentration in lysis buffer, 30 mM imidazole concentration in wash buffer, and 10 column volume wash buffer containing 300 mM salt concentration. This gave a 54% protein recovery improvement over the manufacturer痴 protocol which recorded a product yield of only 7.68 mg/g DCW. The recovered BmSXP recombinant antigen showed good western blot reactivity, high sensitivity (31/32, 97%) and specificity (32/32, 100%) in ELISA, thus attesting to its good purity and quality.

 

 

Indian Journal of Experimental Biology

Vol. 50, April 2012, pp. 265269

 

 

Mosquito cell line C6/36 shows resistance to Cyt1Aa6

Lingling Zhanga, Enjiong Huangb, Baozhen Tanga , Xiong Guana* & Ivan Gelbičc**

aKey Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University,
Fuzhou 350002, People痴 Republic of China

bFujian International Travel Health Care Center, 350001 Fuzhou, Fujian, People痴 Republic of China

cBiological Centre of the Academy of Sciences of the Czech Republic, Institute of Entomology, Brani嗤vsk 31, 37005
Česk Budějovice, Czech Republic

Received 28 November 2011; revised 4 February 2012

This study aimed to investigate the resistance mechanism of C6/36 cells to Cyt1Aa6 protein under selection pressure. Receptor binding properties of Cyt1Aa6 toward sensitive and resistant C6/36 cells were investigated. More sensitive cells were detected with goat-anti-rabbit-FITC-labeled antibody, and the quantity of in vitro activated Cyt1Aa6 toxin bound to resistant cells was greatly reduced. Ligand western blot assays showed that disappearance of the 26 kDa protein and weakness of the positive bands of 68 kDa from resistant cells might lead to the resistance of C6/36 cells to Cyt1Aa6 toxin. The resistance of C6/36 cells was detected under selection in vitro-activated Cyt1Aa6 toxin. Receptor binding demonstrated that reduced Cyt1Aa6 bound to resistant cells, which might be closely related to the disappearance and weakness of some proteins. The results presented here are the first to demonstrate that Cyt1Aa protein, a uniquely characteristic toxin, induced resistance at the cellular level. It might be attributed to the change of receptors.

 

 

Indian Journal of Experimental Biology

Vol. 50, April 2012, pp. 270276

 

 

Immunomodulatory effects of alcoholic and hydroalcoholic extracts of
Moringa olifera Lam leaves

Otilia J F Banji1*, David Banji1 & Kavitha R1

Department of Pharmacology and Toxicology, Nalanda College of Pharmacy, Cherlapally, Nalgonda-508001, India

Received 6 April 2011; revised 6 February 2012

Effects of 50, 100 and 200 mg/kg body weight of the alcoholic and hydro-alcoholic extract of leaves of M. olifera were studied on various immune paradigms like delayed type hypersensitivity reaction using SRBC as an antigen, determination of antibody titer, neutrophil adhesion test as an indicator for neutrophil index, total leucocyte count in cyclophosphamide induced immunosuppressed animals and carbon clearance assay as a measure of phagocytic activity. Hydro-alcoholic extract of M. olifera substantially enhanced cellular immune response, humoral immune response, neutrophil index and phagoctic activity in doses of 100 and 200 mg/kg body weight. The ethanolic extract (200 mg/kg body wieght) was efficient in improving immune response. The results suggest that M. olifera has a significant role to play as an immune stimulator.

 

 

Indian Journal of Experimental Biology

Vol. 50, April 2012, pp. 277281

 

 

Wound healing activity of ethanolic extract of Shorea robusta Gaertn. f. resin

T A Wani, H H Chandrashekara, D Kumar, R Prasad, A Gopal, K K Sardar, S K Tandan & D Kumar*

Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar 243 122 India

Received 7 June 2011; revised 30 January 2012

The ethanolic extract of S. robusta resin (10 and 30 % w/w applied locally in excised and incised wounds) produced a dose-dependent acceleration in wound contraction and increased hydroxyproline content and tensile strength of wounds in rats. The results demonstrate wound healing activity of ethanolic extract of S. robusta resin.

 

 

Indian Journal of Experimental Biology

Vol. 50, April 2012, pp. 282289

 

 

Rapid screening and cultivation of oleaginous microorganisms

Xinlei Gao, Ye Liu & Zhongju Chen

College of Chemical and Environmental Engineering, Wuhan Polytechnic University, Wuhan 430023, China

and

Li Wu*

School of Chemical Engineering and Pharmacy, Hubei Key Lab of Novel Reactor and Green Chemical Technology,
Wuhan Institute of Technology, Wuhan
430073, China

Received 28 June 2011; revised 1February 2012

Oleaginous microbial strains were cultivated to identify the best oil-producing strain amongst Yarrowia lipolytica (CGMCC 2.1398), Lipomyces starkeyi (CGMCC 2.1608), Rhodosporidium toruloides (CGMCC 2.1389), Mortierella isabellina (CGMCC 3.3410), Cunninghamella blakeleana (CGMCC 3.970), and Mycobacterium QJ311. A method for rapid determination of oil content and fatty acid composition was established to identify the optimum oil-producing strains. This method had a relative standard deviation of 4.09%, an average recovery ratio of 97.09% and a detection limit of 0.11.0 g. Mortierella isabellina CGMCC 3.3410 was identified as the best oil-producing strain amongst the six strains tested, with a total biomass of 75 g/10 L and a lipid content of 35%. A rapid screening method of oleaginous microorganisms is discussed for the first time.

 

 

Indian Journal of Experimental Biology

Vol. 50, April 2012, pp. 290299

 

 

Decolourization and degradation of C.I.Reactive Red 195 by
Georgenia sp.CC-NMPT-T3

Madhuri Sahasrabudhe*

Department of Microbiology, Maulana Azad College, Aurangabad, 431 001 India

and

Girish Pathade

Department of Biotechnology, Fergusson College, Pune, 411 004 India

Received 5 July 2011; revised 23 January 2012

Azo dye Reactive Red 195 was selected for decolourization and degradation studies by Georgenia sp.CC-NMPT-T3. Optimization of parameters for dye decolourization was studied under static anoxic condition. Under optimized condition decolourization of Reactive Red 195 by Georgenia sp.CC-NMPT-T3 was found to be 95.93 % at 50 mg/L within five hours in static anoxic condition. The optimum pH and temperature for the decolourization was 7.0 and 40 ーC respectively. The biodegradation was monitored by UV-Vis, and TLC and HPLC. Toxicity study demonstrated no toxicity of the biodegraded product. The results suggest that the isolated organism Georgenia sp.CC-NMPT-T3 as a useful tool to treat wastewater containing reactive dyes.

 

 

Indian Journal of Experimental Biology

Vol. 50, April 2012, pp. 300307

 

 

Cadmium tolerance and antibiotic resistance in Escherichia coli isolated from waste stabilization ponds

Sova Patra, T K Das*, C Avila, V Cabello, F Castillo, D Sarkar (Paria), Susmita Lahiri (Ganguly) & B B Jana**

International Centre for Ecological Engineering

and

*Department of Biochemistry & Biophysics, University of Kalyani, Kalyani 741 235, India

Received 23 March 2009; revised 1 February 2012

The incidence pattern of cadmium tolerance and antibiotics resistance by Escherichia coli was examined periodically from the samples of water, sludge and intestine of fish raised in waste stabilization ponds in a sewage treatment plant. Samples of water and sludge were collected from all the selected ponds and were monitored for total counts of fecal coliform (FC), total coliform (TC) and the population of Escherichia coli, which was also obtained from the intestine of fishes. Total counts of both FC and TC as well as counts of E. coli were markedly reduced from the facultative pond to the last maturation pond. Tolerance limit to cadmium by E. coli tended to decline as the distance of the sewage effluent from the source increased; the effective lethal concentration of cadmium ranged from 0.1 mM in split chamber to 0.05 mM in first maturation pond. E. coli isolated from water, sludge and fish gut were sensitive to seven out of ten antibiotics tested. It appears that holistic functions mediated through the mutualistic growth of micro algae and heterotrophic bacteria in the waste stabilization ponds were responsible for the promotion of water quality and significant reduction of coliform along the sewage effluent gradient.