Indian Journal of Experimental Biology

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VOLUME 50

NUMBER 1

JANUARY 2012

CODEN: IJEB (A6) 50 (1) 1-88 (2012)

ISSN: 0019-5189 (Print); 0975-1009 (Online)

 

CONTENTS

 

 

Papers

 

 

 

Manipulating thyroid status alters endoplasmic reticulum calcium homeostasis in rat cerebellum

7

J T Cole, D C McMullen, W S Kean, A M Yarnell, J J Lucky, M A Selak, J E Buonora,
N E Grunberg, A Verma & W D Watson

 

 

 

Sodium butyrate modulates pRb phosphorylation and induces cell death in human vestibular schwannomas in vitro

19

Rohan Mitra, B Indira Devi, Mohan L Gope, D K Subbakrishna & Rajalakshmi Gope

 

 

 

Ameliorative effect of daidzein: A caveolin-1 inhibitor in vascular endothelium dysfunction induced by ovariectomy

28

Saurabh Sharma, Manjeet Singh & Pyare Lal Sharma

 

 

 

Crosstalk between RXR, LXR and VDR within blood mononuclear cellular model

35

Vivek P Dave, Deepak Kaul & Monika Sharma

 

 

 

Intranasal administration of insulin lowers amyloid-b levels in rat model of diabetes

41

Sarada Subramanian & Mathew John

 

 

 

Modulation of intestinal brush border membrane chemical composition during postnatal development in rats: Effect of gestational diabetes

45

Ruchi Sharma, Shailender Singh Chauhan & Akhtar Mahmood

 

 

 

Interaction of hydroalcoholic extract of Acorus calamus Linn. with sodium valproate and carbamazepine

51

Jatinder Katyal, Vikas Sarangal & Yogendra Kumar Gupta

 

 

 

Effect of Alternanthera brasiliana (L) Kuntze on healing of dermal burn wound

56

Chandana C Barua, Archana Talukdar, Shameem A Begum, Bhaben Buragohain, Jayanti Datta Roy, Debesh C Pathak, Dilip K Sarma, Asheesh K Gupta & Rumi S Bora

 

 

 

Antinociceptive activity of Sesbania sesban (Linn) wood extracts, a preliminary study

61

S A Nirmal, J H Bairagi, A N Patil , S C Pal, C D Upasani & S C Mandal

 

 

 

Optimization of medium composition for the production of mosquitocidal toxins from
Bacillus thuringiensis subsp. israelensis

65

Subbiah Poopathi & B Archana

 

 

 

Secretion of cyclodextrin glucanotransferase in E. coli using Bacillus subtilis lipase signal peptide and optimization of culture medium

72

Dorra Ayadi-Zouari, Radhouane Kammoun, Sonia Jemli, Hichem Chouayekh & Samir Bejar

 

 

 

Staurosporine induced poly (ADP-ribose) polymerase independent cell death in
Dictyostelium discoideum

80

Hina Mir, Jyotika Rajawat & Rasheedunnisa Begum

 

 

 

Information for Authors

87

 

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Editor痴 Note

The Indian Journal of Experimental Biology is covered by the following international abstracting and indexing services:

 

Science Citation Index ExpandedTM

PubMed (http://www.ncbi.nim.nih.gov/)

MEDLINE

BIOSIS

Chemical Abstracts Service

Excerpta Medica

Informascience

Refrativnyi Zhurnal

Zoological Records

 

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Indian Journal of Experimental Biology in Open Access Mode

 

The Indian Journal of Experimental Biology (IJEB) is now an open access journal in the repository, NISCAIR Online Periodicals Repository (NOPR) [http://nopr.niscair.res.in].

Full text of all articles published in IJEB from 2006 onwards can now be accessed at NOPR in the open access mode. Papers in the current issue shall be uploaded immediately. Papers published in earlier years shall be added soon.

NOPR is based on DSpace, a digital repository software, and allows document browsing, document searching and various search options like title, author name, keywords, year, issue, etc.

 

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Author Index

Archana B

65

Ayadi-Zouari Dorra

72

 

 

Bairagi J H

61

Barua Chandana C

56

Begum Rasheedunnisa

80

Begum Shameem A

56

Bejar Samir

72

Bora Rumi S

56

Buonora J E

7

Buragohain Bhaben

56

 

 

Chauhan Shailender Singh

45

Chouayekh Hichem

72

Cole J T

7

 

 

Dave Vivek P

35

Devi B Indira

19

 

 

Gope Mohan L

19

Gope Rajalakshmi

19

Grunberg N E

7

Gupta Asheesh K

56

Gupta Yogendra Kumar

51

Jemli Sonia

72

John Mathew

41

 

 

Kammoun Radhouane

72

Katyal Jatinder

51

Kaul Deepak

35

Kean W S

7

 

 

Lucky J J

7

 

 

Mahmood Akhtar

45

Mandal S C

61

McMullen D C

7

Mir Hina

80

Mitra Rohan

19

 

 

Nirmal S A

61

 

 

Pal S C

61

Pathak Debesh C

56

Patil A N

61

Poopathi Subbiah

65

Rajawat Jyotika

80

Roy Jayanti Datta

56

 

 

Sarangal Vikas

51

Sarma Dilip K

56

Selak M A

7

Sharma Monika

35

Sharma Pyare Lal

28

Sharma Ruchi

45

Sharma Saurabh

28

Singh Manjeet

28

Subbakrishna D K

19

Subramanian Sarada

41

 

 

Talukdar Archana

56

 

 

Upasani C D

61

 

 

Verma A

7

 

 

Watson W D

7

 

 

Yarnell A M

7

 

 

 

Keyword Index

Acorus calamus

51

Alternanthera brasiliana

56

Alzheimer痴 disease

41

Amyloid b

41

Antioxidants

56

Apoptosis

19

Apoptosis inducing factor

80

 

 

Bacillus thuringiensis subsp. israelensis

65

Behavior

7

Benzamide

80

Brain

7

Brush border membrane composition

45

 

 

Calcium

7

Carbamazepine

51

Cathepsin

80

Caveolin

28

Cell death

19

Coconut cake

65

Collagen

56

Cost-effectiveness

65

 

 

Diabetes

41

E. coli

72

Endoplasmic reticulum

7

Estrogen

28

Experimental burn

56

 

 

Gestational diabetes

45

 

 

Hot plate test

61

 

 

Human vestibular schwannoma

19

 

 

Insulin

41

 

 

LXR

35

 

 

MnCl2

65

Mosquito larvae

65

 

 

Naloxone

61

Nuclear receptors

35

 

 

Optimization

72

Ovariectomy

28

 

 

Pentylenetetrazole-induced seizures

51

Pepstatin

80

Postnatal development

45

pRb phosphorylation

19

 

 

Recombinant CGTase

72

Response surface methodology

72

RXR

35

 

 

Secretion

72

SERCA

7

Sesbania sesban

61

Signal peptide

72

Small intestine

45

Sodium butyrate

19

Sodium valproate

51

Staurosporine

80

Streptozotocin

41

 

 

Thyroid hormone

7

Toxicity assays

65

 

 

Vascular endothelium dysfunction

28

VDR

35

 

 

Wound healing

56

Writhing test

61

 

 

 

Correspondent author has been indicated by * sign

 

 

 

Indian Journal of Experimental Biology

Vol. 50, January 2012, pp. 7-18

 

 

Manipulating thyroid status alters endoplasmic reticulum calcium
homeostasis in rat cerebellum

JT Cole1,*,Ŧ, DC McMullen1,Ŧ , WS Kean1, AM Yarnell2, JJ Lucky1, MA Selak1,
JE Buonora1, NE Grunberg 2, A Verma1 & WD Watson1

1Department of Neurology, Uniformed Services University of the Health Sciences,

2Department of Medical and Clinical Psychology, Uniformed Services University of the Health Sciences,
B-3012, 4301 Jones Bridge Road, Bethesda, MD 20814, USA

Received 18 May 2011; revised 24 August 2011

Thyroid-related hormones regulate the efficiency and expression of sarco-endoplasmic reticulum calcium ATPases in cardiac and skeletal muscle. However, little is known about the relationship between thyroid hormones and calcium (Ca2+) homeostasis in the brain. It is hypothesized that manipulating rat thyroid hormone levels would induce significant brain Ca2+ adaptations consistent with clinical findings. Adult male Sprague-Dawley rats were assigned to one of three treatment groups for 28 days: control, hypothyroid (6-n-propyl-2-thiouracil (PTU), an inhibitor of thyroxine (T4) synthesis), and hyperthyroid (T4). Throughout, rats were given weekly behavioral tests. Ca2+ accumulation decreased in the cerebellum in both hyper- and hypothyroid animals. This was specific to different ER pools of calcium with regional heterogeneity in the response to thyroid hormone manipulation. Behavioral tasks demonstrated sensitivity to thyroid manipulation, and corresponded to alterations in calcium homeostasis. Ca2+ accumulation heterogeneity in chronic hyper- and hypothyroid animals potentially explains clinical manifestations of altered thyroid status.

 

 

Indian Journal of Experimental Biology

Vol. 50, January 2012, pp. 19-27

 

 

Sodium butyrate modulates pRb phosphorylation and induces cell death
in human vestibular schwannomas in vitro

Rohan Mitra1, B Indira Devi2, Mohan L Gope4 , D K Subbakrishna3 & Rajalakshmi Gope1*

1Department of Human Genetics, 2Department of Neuro Surgery,
3Department of Biostatistics, NIMHANS, Bangalore 560029, India;
4Department of Biotechnology, Presidency College, Bangalore 560024, India.

Received 18 February 2011; revised 11 August 2011

In the present study, effect of Na-Bu on the pRb phosphorylation was analysed in the primary cultures of 12 VS tumors. Primary cultures of VS tumors were established from the fresh tumor tissues removed surgically and were treated with Na-Bu. Na-Bu treatment for 48 h led to morphological changes and apoptotic cell death in VS tumor cells. Na-Bu treatment decreased level of total pRb and phosphorylated form of pRb and caused specific dephosphorylation at Ser 249/Thr 252 and Ser 567. In the untreated and Na-Bu treated cells (when present), pRb was localised in the nucleus. Moreover, in Na-Bu treated cells the nucleus appeared highly condensed as compared to untreated cells. Results of the present study indicated that Na-Bu treatment modulated pRb phosphorylation status and caused apoptotic cell death in VS tumors.

 

 

Indian Journal of Experimental Biology

Vol. 50, January 2012, pp. 28-34

 

 

Ameliorative effect of daidzein: A caveolin-1 inhibitor in vascular
endothelium dysfunction induced by ovariectomy

Saurabh Sharma, Manjeet Singh and Pyare Lal Sharma*

Cardiovascular Division, Department of Pharmacology, ISF College of Pharmacy,
Moga 142 001, India

Received 17 February 2011; revised 6 September 2011

Estrogen deficiency was produced in female Sprague-Dawley rats by surgical removal of both the ovaries and these animals were used 4 weeks later. Endothelium-dependent and endothelium-independent relaxations due to acetylcholine and sodium nitroprusside were observed respectively, in isolated rat thoracic aortic ring preparation. Extent of lipid peroxidation was measured by estimating serum TBARS. Integrity of vascular endothelium was assessed using hematoxylin and eosin staining. Generation of nitric oxide was measured indirectly, by estimating serum and urinary nitrite/nitrate concentration. Ovariectomy produced significant vascular endothelial dysfunction, measured in terms of reduced acetylcholine-induced endothelium-dependent vasorelaxation, serum and urinary nitrite/nitrate concentration and impairment of integrity of vascular endothelium. Administration of daidzein (0.2 mgkg-1day-1, sc 0.4 mgkg-1day-1, sc and 0.8 mgkg-1day-1, sc) and Atorvastatin (30 mgkg-1day-1, po Positive Control) for one week markedly improved vascular endothelial dysfunction due to increase in nitric oxide bioavailability perhaps by inhibiting caveolin-1 and activation of PI3K-AKT pathway.

 

 

Indian Journal of Experimental Biology

Vol. 50, January 2012, pp. 35-40

 

 

Crosstalk between RXR, LXR and VDR within blood mononuclear cellular model

Vivek P Dave, Deepak Kaul* & Monika Sharma

Department of Experimental Medicine and Biotechnology,
Post Graduate Institute of Medical Education and Research Chandigarh, 160 012, India

Received 14 January 2011; revised 19 September 2011

An accumulation of data from in vitro to in vivo model system has established a pivotal role of three crucial ligand activated nuclear receptors RXR, LXR-α and VDR for their ability to regulate an array of genes involved in regulation of fundamental cellular processes to patho-physiological situations. Keeping in view RXR as a common heterodimeric partner for LXR-α and VDR, the present study was designed to dissect the interrelationship between these three nuclear receptors in peripheral blood mononuclear cellular model. The present study revealed that all the three nuclear receptors displayed auto regulation in response to their specific ligands; Both LXR-α and VDR regulated the expression of their heterodimeric partner RXR; and VDR was regulated by LXR-α through its ability to modulate SREBP response element present in the promoter region of VDR gene. Based on these findings, the role of these nuclear receptors could be better understood in various nuclear receptor mediated pathological processes.

 

 

Indian Journal of Experimental Biology

Vol. 50, January 2012, pp. 41-44

 

 

Intranasal administration of insulin lowers amyloid-゚ levels in
rat model of diabetes

Sarada Subramanian* & Mathew John

Department of Neurochemistry, National Institute of Mental Health & Neurosciences, Hosur Road, Bangalore 560 029, India

Received 12 January 2011; revised 8 September 2011

Alzheimer痴 disease (AD) is a neurodegenerative disorder characterized by abnormal accumulation of amyloid ゚ (A゚) peptide in brain regions subserving memory and other cognitive functions. Hyperglycemia and perturbed insulin signaling have been proposed as pathogenic factors contributing to AD. The aim of the present study is to validate the use of streptozotocin (STZ) injected rats as an experimental model of AD. Using this model, the effect of intranasal administration of insulin on reduction of A゚ levels was measured. The current findings strengthen the case for insulin as therapy for AD afflicted individuals with or without diabetes.

 

Indian Journal of Experimental Biology

Vol. 50, January 2012, pp. 45-50

 

 

Modulation of intestinal brush border membrane chemical composition during postnatal development in rats: Effect of gestational diabetes

Ruchi Sharma1, Shailender Singh Chauhan2 & Akhtar Mahmood2*

1 Department of Biotechnology, Government College for Girls, Chandigarh, 160 036, India

2 Department of Biochemistry, Panjab University, Chandigarh, 160 014, India

Received 15 July 2011; revised 26 September 2011

There was a significant increase in fucose (52%), total hexoses (16%) and hexosamine (56%) except sialic acid, which was reduced (77%) in the microvillus membrane of infants born to rat mothers made diabetic by injecting alloxan on day 3 of gestation. Expressed on the protein basis there were a significant increase in membrane, triglyceride, total cholesterol, and phospholipids content of brush border in pups from diabetic group between 5-45 days of postnatal age. Intestinal morphology in diabetic group showed, regression of tubular glands, distorted cellular organization of mucosal cells, reduction in the mucosal cell height and number of secretory goblet cells. These findings suggest that the gestational diabetes affects the sugar and lipid composition of the intestinal brush border membrane in rats during early stages of the postnatal development, which may be associated with compromised tissue functions later in life.

 

 

Indian Journal of Experimental Biology

Vol. 50, January 2012, pp. 51-55

 

 

Interaction of hydroalcoholic extract of Acorus calamus Linn. with sodium valproate and carbamazepine

Jatinder Katyal*, Vikas Sarangal & Yogendra Kumar Gupta

Neuropharmacology Laboratory, Department of Pharmacology, All India Institute of Medical Sciences,
New Delhi 110 029, India

Received 13 May 2011; revised 29 August 2011

Anticonvulsant property of Acorus calamus is known. Since combination therapy can lower the dose of individual drug and dose related toxicities, in this study, the effect of co-administration of hydroalcoholic extract of A. calamus (HAEAC) on conventional antiepileptic drugs (AEDs), sodium valproate and carbamazepine was determined using pentylenetetrazole -induced seizures model in rats. On combining the subanticonvulsant doses of HAEAC with sodium valproate and carbamazepine, greater protection as compared to either drug alone was observed. This was not related to change in levels of the AEDs. Thus, the results further substantiate anticonvulsant effect of HAEAC and suggest a potential for add on therapy with AEDs.

 

 

Indian Journal of Experimental Biology

Vol. 50, January 2012, pp. 56-60

 

 

Effect of Alternanthera brasiliana (L) Kuntze on healing of dermal burn wound

Chandana C Barua1,* Archana Talukdar1, Shameem A Begum1, Bhaben Buragohain1, Jayanti Datta Roy1,
Debesh C Pathak2, Dilip K Sarma3, Asheesh K Gupta & Rumi S Bora4

Department of 1Veterinary Pharmacology and Toxicology, 2Veterinary Pathology, 3Veterinary Microbiology and 4Animal Production & Management, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati 781 022, India
and
Department of Biochemical Pharmacology, Defence Institute of Physiology and Allied Sciences, Delhi 110 054, India

Received 25 March 2011; revised 11 October 2011

Wound healing activity of methanol extract of Alternanthera brasiliana [5% (w/w) ointment] was evaluated in experimental burn wound model in rats. Healing potential was assessed by the rate of wound contraction, estimation of anti-oxidants like catalase, superoxide dismutase, reduced glutathione, protein, vitamin C and hydroxyproline, along with histopathological examination on 8th day post wounding. The statistical data indicated that there was significant increase in wound contraction along with augmented level of antioxidants in granulation tissues in A. brasiliana treated group. Histopathological assessment of the granulation tissue revealed formation of epidermis with keratin layer and deposition of collagen fibers after treatment with the plant extract.

 

 

Indian Journal of Experimental Biology

Vol. 50, January 2012, pp. 61-64

 

 

Antinociceptive activity of Sesbania sesban (Linn) wood extracts,
a preliminary study

S A Nirmal1*, J H Bairagi1, A N Patil1, S C Pal2, C D Upasani3 & S C Mandal4

1Department of Pharmacognosy, Pravara Rural College of Pharmacy, Loni, 413 736, India

2Department of Pharmacognosy, NDMVP College of Pharmacy, Nasik, 422 002, India

3Department of Pharmacology, SSJ College of Pharmacy, Chandawad, Nashik, 423 101, India

4Pharmacognosy and Phytotherapy Research Laboratory, Department of Pharmaceutical Technology,
Jadavpur University, Kolkata, 700 032, India

Received 4 April 2011; revised 2 September 2011

The wood of the plant Sesbania sesban, is reported to have antinociceptive activity. To validate its folk use in the treatment of pain, wood was extracted successively with petroleum ether, chloroform, ethyl acetate, ethanol, and water to produce respective extracts. The extracts (50 and 100 mg/kg, ip) were screened for antinociceptive activity using hot plate test and acetic acid-induced writhing test in mice. Petroleum ether, chloroform, and ethyl acetate extracts showed significant and dose-dependent activity in both the tests. In order to find out the involvement of opioid receptors, effect of naloxone
(1 mg/kg, sc) on the action of extracts was checked in hot plate test. Petroleum ether, chloroform, and ethyl acetate extracts showed significant and dose dependant antinociceptive activity. The antinociceptive action of the extracts was blocked by naloxone, suggesting involvement of opioid receptors in the action.

 

 

 

Indian Journal of Experimental Biology

Vol. 50, January 2012, pp. 65-71

 

 

Optimization of medium composition for the production of mosquitocidal toxins from Bacillus thuringiensis subsp. israelensis

Subbiah Poopathi* & B Archana

Unit of Microbiology and Immunology, Vector Control Research Centre (ICMR)
Ministry of Health and Family Welfare, Government of India, Indira Nagar, Pondicherry 60 5006, India

Received 16 May 2011; revised 26 September 2011

Optimization of chicken feather (CF) based culture medium for the production of Bacillus thuringiensis subsp. israelensis (Bti) biomass in combination with the agro industrial by-product (coconut cake, CC) and manganese chloride (MnCl2) has been evaluated. The biomass yield of Bti spore/crystal toxin was highest (12.06 g/L) from the test medium (CF+CC+MnCl2) compared to the reference medium (Luria Bertani, LB). Toxicity assay with Bti produced from the test medium against mosquito vectors (Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti) was also satisfactory and results were comparable with bacteria produced from LB. The results suggest that Bti can be produced to the maximum extent possible as a potential mosquitocidal activity as suggested by the test medium (CF+CC+MnCl2).

 

 

Indian Journal of Experimental Biology

Vol. 50, January 2012, pp. 72-79

 

 

Secretion of cyclodextrin glucanotransferase in E. coli using Bacillus subtilis lipase signal peptide and optimization of culture medium

Dorra Ayadi-Zouari*, Radhouane Kammoun, Sonia Jemli, Hichem Chouayekh & Samir Bejar

Laboratory of Microorganisms and Biomolecules, Centre of Biotechnology of Sfax, Universit de Sfax, BP 1177 Sfax, Tunisia

Received 18 May 2011; revised 23 August 2011

The cyclodextrin glycosyltransferase (CGTase) of Paenibacillus pabuli US132 was fused to the secretive lipase signal peptide of B. subtilis. This leads to an efficient secretion of the recombinant enzyme into the culture medium of E. coli as an active and soluble form contrasting with the native construction leading to a periplasmic production. In order to enhance the yield of CGTase production, an experimental design methodology was applied for the optimization of the culture composition. Hence, the media components were submitted to preliminary screening using a Plakett-Burman design. The concentrations of the major operating ones were then optimized to enhance the secretion of CGTase using response surface methodology. The findings revealed that concentrations of 0.5% potato starch, 3% yeast extract, 3% tryptone, 1.5% casein hydrolysate, 0.5% NaCl, 0.2% KH2PO4, and 0.02% MgSO4 were the optimal conditions for CGTase production. The experimental value (9.43 U/ml) obtained for CGTase activity was very close to the predicted value (9.27 U/ml).

 

 

 

Indian Journal of Experimental Biology

Vol. 50, January 2012, pp. 80-86

 

 

Staurosporine induced poly (ADP-ribose) polymerase independent cell death in Dictyostelium discoideum

Hina Mir, Jyotika Rajawat & Rasheedunnisa Begum*

Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of

Baroda, Vadodara 390 002, India

Received 20 May 2011; revised 9 August 2011

In the present study D. discoideum has been used as a model organism to understand the role of poly (ADP-ribose) polymerase (PARP) in caspase independent paraptotic cell death pathways. D. discoideum lacks caspases and Bcl-2 family proteins; nevertheless it has 9 potential genes for PARP. PARP has been known to get activated in various cell death associated diseases. In this study kinetics of cell death induced by staurosporine (STS), a bacterial alkaloid, was established to unravel the role of PARP. It was found that STS induced cell death in D. discoideum did not involve PARP activation, however it involved cathepsin D. Results indicated that an alternative mechanism may be existing in D. discoideum that lacks Bcl-2 family proteins for STS induced cell death that evades Bax involvement.

 

 

 

Indian Journal of Experimental Biology

Vol. 50, January 2012, pp. 87-88

 

 

 

Information for Authors