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Indian Journal of Experimental Biology http://www.niscair.res.in; http://nopr.niscair.res.in
Total visitors: 1290 since 15-05-2012
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VOLUME 50 |
NUMBER 5 |
MAY 2012 |
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CODEN: IJEB (A6) 50 (4) 309-378 (2012) |
ISSN: 0019-5189 (Print);
0975-1009 (Online) |
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CONTENTS |
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Papers |
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Anti-HCMV and
KSHV effect of a trapping ligand antagonist for Herpesvirus-encoded GPCR Hong-Ai Liu,
Han-Xiao Sun*, Xue-Mei Mo, Shi-Yu Li, Xiu-Ying Li, Guang Zhang & Lu Li
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313 |
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Nimodipine
down-regulates CGRP expression in the rat trigeminal nucleus caudalis |
320 |
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Prokaryotic
expression of chicken infectious anemia apoptin protein and characterization
of its polyclonal antibodies Shikha
Saxena, Gandham Ravi Kumar, Prafull Singh, Uttara Chaturvedi, Lovleen Saxena,
Rajiv
Kumar, Aditya Prasad Sahoo, Juwar Doley, Rajmani, Amit Kumar, Sudesh Kumar
&
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325 |
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Effect
of supplementation of dermal fibroblasts conditioned medium on expansion of keratinocytes through
enhancing attachment |
332 |
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Phenotypic
and molecular characterization of indigenous rhizobia nodulating chickpea in Rhitu Rai,
Prasanta K Dash, Trilochan Mohapatra & Aqbal Singh |
340 |
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Antihyperglycemic activity of Woodfordia fruticosa (Kurz)
flowers extracts in glucose metabolism
and lipid peroxidation in streptozotocin-induced diabetic rats Neeraj
Verma, G Amresh1, P K Sahu, Ch V Rao & Anil Pratap Singh
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351 |
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Antitumor
potential of Castanopsis indica (Roxb. ex Lindl.) A. DC. leaf extract against
Ehrlich's ascites
carcinoma cell Narayan
Dolai, Indrajit Karmakar, R B Suresh Kumar, Asis Bala, U K Mazumder &
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359 |
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Effect of
temperature on behavioural isolation : A study with Drosophila ananassae
populations |
366 |
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Nitric oxide alleviates oxidative damage
induced by high temperature stress in wheat |
372 |
Editor’s Note
The Indian Journal of Experimental Biology is
covered by the following international abstracting and indexing services:
Science Citation Index
ExpandedTM
PubMed
(http://www.ncbi.nim.nih.gov/)
MEDLINE
BIOSIS
Chemical Abstracts Service
Excerpta Medica
Informascience
Refrativnyi Zhurnal
Zoological Records
————————————————————
Indian Journal of Experimental Biology in Open Access
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The Indian Journal of Experimental Biology
(IJEB) is now an open access journal in the repository, NISCAIR Online
Periodicals Repository (NOPR) [http://nopr.niscair.res.in].
Full text of all articles published in IJEB from 2006
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Author Index |
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Aminuddin B S |
332 |
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Amresh G |
351 |
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Bala Asis |
359 |
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Bansal Devanshu |
320 |
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Bavita A |
372 |
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Chaturvedi Uttara |
325 |
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Chowdhury S R |
332 |
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Dash Prasanta K |
340 |
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Dolai Narayan |
359 |
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Doley Juwar |
325 |
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Haldar Pallab Kanti |
359 |
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Karmakar Indrajit |
359 |
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Kumar Amit |
325 |
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Kumar Gandham |
325 |
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Kumar R.B.Suresh |
359 |
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Kumar Rajiv |
325 |
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Kumar Sudesh |
325 |
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Li Lu |
313 |
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Li Shi-Yu |
313 |
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Li Xiu-Ying |
313 |
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Liu Hong-Ai |
313 |
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Mazumder U. K. |
359 |
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Mo Xue-Mei |
313 |
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Mohapatra Trilochan |
340 |
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Navtej S B |
372 |
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Rai Rhitu |
340 |
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Rajmani |
325 |
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Rao Ch V |
351 |
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Ray Subrata Basu |
320 |
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Ruszymah B H I |
332 |
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Sahoo Aditya Prasad |
325 |
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Sahu P K |
351 |
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Saxena Lovleen |
325 |
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Saxena Shikha |
325 |
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Shashi B |
372 |
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Singh Anil Pratap |
351 |
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Singh Aqbal |
340 |
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Singh Prafull |
325 |
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Sun Han-Xiao |
313 |
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Tiwari Ashok K |
325 |
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Verma Neeraj |
351 |
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Vijayan Lakshmy |
320 |
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Yadav Anand Kumar |
366 |
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Yadav Sujata |
366 |
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Zhang Guang |
313 |
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Keyword Index |
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Antagonist |
313 |
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Antihyperglycemic |
351 |
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Antioxidant |
351,359 |
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Antioxidant enzymes |
372 |
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Antitumor |
359 |
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Behavioural isolation |
366 |
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Calcium channel blocker |
320 |
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cAMP response-element |
320 |
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Castanopsis indica |
359 |
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Chicken infectious anemia |
325 |
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Chickpea |
340 |
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Cytotoxicity |
359 |
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Dermal fibroblast conditioned medium |
332 |
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Diabetes mellitus |
351 |
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Drosophila ananassae |
366 |
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EAC cell |
359 |
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Efficiency of cell |
332 |
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Expansion index |
332 |
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Genetic diversity |
340 |
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High temperature stress |
372 |
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Human cytomegalovirus |
313 |
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Image J |
320 |
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Immunohistochemistry |
320 |
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Kaposi’s sarcoma- |
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Keratinocytes |
332 |
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Lipid peroxidation |
372 |
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Migraine |
320 |
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Peptide H22-LP |
313 |
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Phylogeny |
340 |
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Polyclonal sera |
325 |
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Prokaryotic
expression |
325 |
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Rhizobia |
340 |
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Sodium
nitroprusside |
372 |
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Streptozotocin |
351 |
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Symbiotic efficiency |
340 |
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Temperature effect |
366 |
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Tissue Engineering |
332 |
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Trapping receptor/ligand |
313 |
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Trigeminal ganglia |
320 |
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US28 |
313 |
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VP3 gene |
325 |
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Wheat |
372 |
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Woodfordia fruticosa |
351 |
Correspondent author has been indicated by * sign
Indian Journal of Experimental Biology
Vol. 50, May 2012, pp. 313-319
Anti-HCMV and KSHV
effect of a trapping ligand antagonist for
Herpesvirus-encoded GPCR
Hong-Ai Liu, Han-Xiao Sun*, Xue-Mei Mo, Shi-Yu Li, Xiu-Ying Li, Guang Zhang & Lu Li
Institute of Genomic Medicine,
Received 28 September 2011; revised 28 February 2012
We have found and synthesized a trapping
ligand peptide H22-LP (the conservative sequence is
NAHCALL) from a random phage library according to the broad-spectrum trapping
receptor H22, which derived from the residue 14-35 near the N-terminal region
of receptor US28 on HCMV. In this study, we will evaluate its potential as an
efficient antagonist of US28 and the anti-virus activity, acting as a broad
spectrum chemokine receptors antagonist. Stable expression of US28 and ORF
Indian Journal of Experimental Biology
Vol. 50, May 2012, pp. 320-324
Nimodipine down-regulates
CGRP expression in the rat
trigeminal nucleus caudalis
Lakshmy Vijayan, Devanshu Bansal & Subrata Basu Ray*
Department of
Anatomy, All India Institute of Medical Sciences (AIIMS),
Received 15 July 2011; revised 6 February 2012
L-type calcium channel blockers like
verapamil are used in the prophylaxis of migraine. However, their effect on the
expression of CGRP in the trigeminal nucleus caudalis (TNC) is unknown. It is
important because an earlier study had shown that olcegepant, a CGRP receptor
antagonist, acts at the level of the trigeminal spinal nucleus rather than the
trigeminal ganglia. Nimodipine was used in the present study as it crosses the
blood-brain barrier. The objective of the study was to determine the pattern of
expression of calcitonin gene-related peptide (CGRP) in the TNC after
administration of nimodipine and/or morphine. Wistar rats were injected with
saline, morphine, nimodipine or morphine + nimodipine for 14 days.
Subsequently, the lowest part of the medulla oblongata containing the spinal
nucleus was removed and processed for immunohistochemical localization of CGRP.
The density of expression was quantified using Image J software. The results
were statistically analyzed. CGRP expression was noted over the superficial
part of the TNC, which decreased significantly after nimodipine administration.
Conversely, morphine produced an up-regulation. The expression was unchanged
with reference to saline in the morphine + nimodipine treated group. Decreased
expression of CGRP in the trigeminal nucleus caudalis after nimodipine is being
reported for the first time. Also, whether CGRP expression can be used as a
marker for predicting the therapeutic efficacy of an anti-migraine drug is
currently being investigated.
Indian Journal of Experimental Biology
Vol. 50, May 2012, pp. 325-331
Prokaryotic expression of chicken infectious anemia apoptin protein and characterization of its polyclonal antibodies
Shikha Saxena, Gandham Ravi Kumar, Prafull
Singh, Uttara Chaturvedi, Lovleen Saxena, Rajiv Kumar,
Aditya Prasad Sahoo , Juwar Doley, Rajmani, Amit Kumar, Sudesh Kumar
& Ashok K Tiwari*
Molecular Biology
Laboratory, Department of Veterinary Biotechnology,
Indian Veterinary Research Institute,
Izatnagar, 243 122,
Received 2 June 201; revised 21 February 2012
In the present
study recombinant VP3 (rVP3) was expressed in E.coli BL21 (DE3) (pLysS) and its polyclonal antibodies were
characterized. SDS–PAGE analysis revealed that the expression of recombinant
protein was maximum when induced with 1.5 mM
IPTG for 6 h at 37ºC. The 6×His-tagged fusion protein was purified on Ni-NTA
and confirmed by Western blot using CAV specific antiserum. Rabbits were
immunized with purified rVP3 to raise anti-VP3 polyclonal antibodies.
Polyclonal serum was tested for specificity and used for confirming expression
of VP3 in HeLa cells transfected with pcDNA.cav.vp3 by indirect fluorescent
antibody test (IFAT), flow cytometry and Western blot. Available purified rVP3
and polyclonal antibodies against VP3 may be useful to understand its functions
which may lead to application of VP3 in cancer therapeutics.
Indian Journal of Experimental Biology
Vol. 50, May 2012, pp. 332-339
Effect of supplementation of dermal
fibroblasts conditioned medium on
expansion of keratinocytes through enhancing attachment
S R Chowdhury1, B
1Tissue Engineering Centre, Universiti Kebangsaan
Jalan Yaacob Latif, Bandar
Tun Razak, Cheras, 56000
2Ear, Nose and Throat Consultant Clinic,
Taman Dato’ Ahmad Razali, 68000
3Department of Physiology, Universiti Kebangsaan
Jalan Raja Muda
Received 28 June 2011; revised 27 February 2012
In the present
study in vitro expansion of human keratinocytes by supplementing dermal
fibroblasts conditioned medium (DFCM) has been reported. Effect of two
different DFCM acquired by culturing fibroblasts in keratinocyte-specific
medium (defined keratinocytes serum free medium, DFCM-DKSFM) and
fibroblast-specific serum free medium (F12: DMEM nutrient mix, DFCM-FD) have
been compared. Growth kinetics of keratinocytes in terms of efficiency of cell
attachment, expansion index, apparent specific growth rate and growth potential
at the end of culture was evaluated in culture supplemented with DFCM-DKSFM and
DFCM-FD in comparison with control i.e. DKSFM only. Results indicated that supplementation
of DFCM caused significant increase in keratinocyte attachment. Efficiency of
keratinocyte attachment in culture supplemented with DFCM-DKSFM was
significantly higher compared to those cultured in DFCM-FD and DKSFM. In addition, the
expansion index of keratinocytes in cultures supplemented with DFCM-DKSFM and
DFCM-FD were 3.7 and 2.2 times higher than that of control condition even
though the apparent growth rate and proliferative potential was found
significantly lower. These results suggested that supplementation of DFCM
enhanced expansion of keratinocyte by increasing efficiency of cell attachment,
and DFCM-DKSFM provided suitable condition for in vitro expansion of
keratinocytes compared to DFCM-FD and control condition.
Indian Journal of Experimental Biology
Vol. 50, May 2012, pp. 340-350
Phenotypic and molecular characterization
of indigenous rhizobia nodulating chickpea in
Rhitu Rai*, Prasanta K Dash, Trilochan Mohapatra & Aqbal Singh
National Research Centre on Plant Biotechnology, Indian Agricultural
Research Institute Campus,
Received 20 July 2011; revised 6 February 2012
In a combined approach of phenotypic and
genotypic characterization, 28 indigenous rhizobial isolates obtained
from different chickpea growing regions in peninsular and northern
Indian Journal of Experimental Biology
Vol. 50, May 2012, pp. 351-358
Antihyperglycemic activity of Woodfordia
fruticosa (Kurz) flowers
extracts in glucose metabolism and lipid peroxidation in
streptozotocin-induced diabetic rats
Neeraj Verma1*, G Amresh1, P K Sahu2, Ch V Rao3 & Anil Pratap Singh3
1Department of Pharmacology, Goel Institute of Pharmacy &
Sciences,
2Department of Pharmacology,
Kalinga Nagar, Ghatikia,
3Pharmacognosy and Ethnopharmacology Division, CSIR-National
Botanical Research Institute,
Rana Pratap Marg, P.O. Box No. 436,
Received 28 June 2011; revised 14 February 2012
The ethanolic extract of W. fruticosa flowers (250 and 500 mg/kg) significantly reduced fasting blood glucose level and increased insulin level after 21 days treatment in streptozotocin diabetic rats. The extract also increased catalase, superoxide dismutase, glutathione reductase, glutathione peroxidase activities significantly and reduced lipid peroxidation. Glycolytic enzymes showed a significant increase in their levels while a significant decrease was observed in the levels of the gluconeogenic enzymes in ethanolic extract treated diabetic rats. The extract has a favourable effect on the histopathological changes of the pancreatic β- cells in streptozotocin induced diabetic rats. The results suggest that W. fruticosa possess potential antihyperglycemic effect by regulating glucose homeostasis and antioxidant efficacy in streptozotocin-induced diabetic rats.
Indian Journal of Experimental Biology
Vol. 50, May 2012, pp. 359-365
Antitumor potential of Castanopsis indica (Roxb. ex Lindl.) A. DC. leaf extract against Ehrlich's ascites carcinoma cell
Narayan Dolai1,
Indrajit Karmakar2, R B Suresh Kumar3, Asis Bala2,3,
U K Mazumder3 &
Pallab Kanti Haldar2,3,*
1Department of Natural Products, National Institute of Pharmaceutical
Education and Research, Kolkata 700 032,
2Department of Pharmacology, Himalayan Pharmacy Institute, Majhitar,
East Sikkim 737 136,
3Department of Pharmaceutical Technology,
Received 5 August 2011; revised 29 February 2012
Methanol extract
of C. indica (MECI) leaves showed direct
cytotoxicity on Ehrlich ascites carcinoma (EAC) cell in a dose dependant manner
and there was significant decrease in the tumor volume, viable cell count,
tumor weight and elevated the life span of EAC tumor bearing mice. Hematological
profile and biochemical
estimations were significantly restored to normal levels in MECI treated as compared to EAC control mice. MECI treatment significantly
modulated the tissue antioxidant assay parameters as
compared to the EAC control mice. The results revealed that MECI possesses
significant dose dependent antitumor potential which may be due to its
cytotoxicity and antioxidant properties.
Indian Journal of Experimental Biology
Vol. 50, May 2012, pp. 366-371
Effect of temperature on behavioural
isolation : A study with
Drosophila ananassae populations
Sujata Yadav* & Anand Kumar Yadav
Department of Zoology,
Received 1 August 2011; revised 17 February 2012
Experiments were conducted to study sexual isolation among two natural populations of Drosophila ananassae maintained at 18 °C and 24 °C for 12 generations in the laboratory to see the effect of this environmental variable on behavioural isolation. Multiple choice technique was used and matings were observed directly in Elens Wattiaux mating chamber. Results showed sexual isolation among strains that were maintained at different temperatures, indicating that temperature may have affected the mating behaviour of the flies which resulted in the induction of ethological isolation among the strains.
Indian Journal of Experimental Biology
Vol. 50, May 2012, pp. 372-378
Nitric oxide alleviates oxidative damage induced by
high temperature
stress in wheat
A Bavita1*, B Shashi1 & S B Navtej2
1Department of Biochemistry, 2Department
of Plant Breeding and Genetics
Punjab Agricultural University,
Received 27 September 2011; revised 18 February 2012
Effect of
sodium nitroprusside (SNP), a donor of nitric oxide (NO) was examined in two
wheat (Triticum aestivum L.)
cultivars, C 306 (heat tolerant) and PBW 550 (comparatively heat susceptible)
to study the extent of oxidative injury and activities of antioxidant enzyme in
relation to high temperature (HT) stress. HT stress resulted in a marked
decrease in membrane thermostability (MTS) and 2, 3, 5-triphenyl tetrazolium
chloride (TTC) cell viability whereas content of lipid peroxide increased in
both the cultivars. The tolerant cultivar C 306 registered less damage to
cellular membranes compared to PBW 550 under HT stress. Activities of
antioxidant enzymes viz, superoxide dismutase, catalase, ascorbate peroxidase,
guaicol peroxidase and glutathione reductase increased with HT in both the
cultivars. Following treatment with SNP, activities of all antioxidant enzymes
further increased in correspondence with an increase in MTS and TTC.
Apparently, lipid peroxide content was reduced by SNP more in shoots of heat
tolerant cultivar C 306 indicating better protection over roots under HT
stress. The up-regulation of the antioxidant system by NO possibly contributed
to better tolerance against HT induced oxidative damage in wheat.