Indian Journal of Experimental Biology

http://www.niscair.res.in; http://nopr.niscair.res.in

 

Total visitors: 4772 since 29-08-2012

 

 

VOLUME 50

NUMBER 9

SEPTEMBER 2012

CODEN: IJEB (A6) 50 (9) 591-664 (2012)

ISSN: 0019-5189 (Print); 0975-1009 (Online)

 

CONTENTS

 

Review Article

 

 

 

Regulation of telomerase activity in ovarian granulosa cells

595

 

 

Ewa Chronowska

 

 

 

Papers

 

 

 

Effect of methanolic and water extract of Leucobryum bowringii Mitt. on
growth, migration and invasion of MCF 7 human breast cancer cells in vitro

602

 

 

G S Manoj , T R Santhosh Kumar, Saneesh Varghese & K Murugan

 

 

 

Polysaccharide enriched immunomodulatory fractions from Tinospora cordifolia (Willd) miers ax hook. F. & Thoms

612

 

 

Upendra Sharma, Manju Bala, Rikki Saini, Praveen Kumar Verma, Neeraj Kumar, Bikram Singh, Renuka Kulkarni Munshi & Supriya Bhalerao

 

 

 

In vitro expression studies of non structural 1 protein of Canine Parvo virus 2 by polyclonal antiserum raised against CPV-NS1 protein expressed in Escherichia coli as an antigen

618

 

 

G Ravi Kumar, Shikha Saxena, Lovleen Saxena, Uttara Chaturvedi, Lakshman Santra, Rajiv Kumar, Aditya Prasad Sahoo, Rajmani, Aswani Kumar, G S Desai, Sudesh Kumar & Ashok K Tiwari

 

 

 

Antidepressant and anxiolytic-like effects of 4n, a novel 5-HT3 receptor antagonist using behaviour based rodent models

625

 

 

Baldev Kumar, Ankur Jindal, Dilip Kumar Pandey, Shvetank Bhatt, Thangaraj Devadoss & Radhakrishnan Mahesh

 

 

 

Role of C-fibers during acute and chronic stress on formalin-induced paw edema in rats

633

Zahra Sepehri, Masoud Fereidoni & Saeed Niazmand

 

 

 

Protective effects of subchronic caffeine administration on cisplatin induced urogenital toxicity in male mice

638

M Khazaei, P D Bayat, A Ghanbari, S Khazaei , M Feizian, A Khodaei, H A S Alian

 

 

 

Thirteen-week oral toxicity study of carotenoid pigment from Rhodotorula glutinis DFR-PDY in rats

645

 

 

B V Latha & K Jeevaratanm

 

 

 

Functional ureogenesis and changes of amino acid metabolism in amphihaline shad hilsa (Tenualosa ilisha, Hamilton-Buchanan) while inhabiting in estuary and freshwater habitats

652

 

 

Umesh C Goswami, Makibur Rahman & Nirmalendu Saha

 

 

 

Eclosion behaviour of three species of Drosophila under different light regimes

660

 

 

Shereen Kouser & V. Shakunthala

 

 

 

Editor痴 Note

The Indian Journal of Experimental Biology is covered by the following international abstracting and indexing services:

 

Science Citation Index ExpandedTM

PubMed (http://www.ncbi.nim.nih.gov/)

MEDLINE

BIOSIS

Chemical Abstracts Service

Excerpta Medica

Informascience

Refrativnyi Zhurnal

Zoological Records

覧覧覧覧覧覧覧

 

NISCAIR痴 Policy on Plagiarism

 

The system of formal communication in science through publication in primary journals is based on originality and quality of information being the only criteria for publication. However, there have been tendencies to misuse the system and vitiate the process of science communication for personal benefits. One of the ills afflicting science communication is plagiarism. Attempts at plagiarism may range from verbatim, copying of extensive material of other authors, misappropriating results/data of others with minor changes in language/presentation without giving credit to original source, to publish essentially the same information more than once.

As the premier publisher in India of primary scientific journals in various disciplines of science and technology, NISCAIR strongly reiterates its policy of discouraging plagiarism of all kinds. All efforts are made detect and frustrate attempts at plagiarism through editorial screening and rigorous peer review in respect of communications received for publication in NISCAIR publications. Cooperation of the scientific community is sought in our efforts to frustrate all attempts at plagiarism.

In case any attempt to plagiarize is brought to our attention accompanied with convincing evidence, following steps would be taken:

(a)                After consulting the respective Editorial Board Members, authors guilty of plagiarism will be debarred from publishing their papers in NISCAIR journals

(b)               Heads of the departments/institutes of the offending authors will be intimated of such incidences of plagiarism.

(c)                Such incidents of plagiarism will be publicized through the concerned NISCAIR journals in consultation with the respective Editorial Board Members.

 

 

 

Author Index

Alian H A S

638

Aswani Kumar

618

 

 

Baldev Kumar

625

Bayat P D

638

Bhalerao Supriya

612

Bhatt Shvetank

625

 

 

Chaturvedi Uttara

618

Chronowska Ewa

595

 

 

Desai G S

618

Devadoss Thangaraj

625

 

 

Feizian M

638

Fereidoni Masoud

633

 

 

Ghanbari A

638

Goswami Umesh C

652

 

 

Jeevaratanm K

645

Jindal Ankur

625

Khazaei M

638

Khazaei S

638

Khodaei A

638

Kouser Shereen

660

 

 

Latha B V

645

 

 

Mahesh Radhakrishnan

625

Manju Bala

612

Manoj G S

602

Munshi Renuka Kulkarni

612

Murugan K

602

 

 

Neeraj Kumar

612

Niazmand Saeed

633

 

 

Pandey Dilip Kumar

625

 

 

Rahman Makibur

652

Rajiv Kumar

618

Rajmani

618

Ravi Kumar G

618

 

 

Saha Nirmalendu

652

Sahoo Aditya Prasad

618

Saini Rikki

612

Santhosh Kumar T R

602

Santra Lakshman

618

Saxena Lovleen

618

Saxena Shikha

618

Sepehri Zahra

633

Shakunthala V

660

Sharma Upendra

612

Singh Bikram

612

Sudesh Kumar

618

 

 

Tiwari Ashok K

618

 

 

Varghese Saneesh

602

Verma Praveen Kumar

612

 

 

 

Keyword Index

5-HT receptor antagonist

625

Adhesion

602

Amino acid metabolism

652

Ammonia

652

Antioxidant property

645

Apoptosis

638

 

 

Breast cancer cells

602

 

 

Caffeine

638

Canine Parvo virus

618

Capsaicin

633

Carotenoids

645

C-fiber

633

Cisplatin

638

 

 

Depression

625

Dexamethasone

633

Differentiation

595

 

 

Eclosion behaviour

660

Endogenous

660

Environmental salinity

652

Follicle

595

Forced swim test

625

 

 

Granulosa cells

595

 

 

Immunomodulatory activity


612

Inflammation

633

Invasion

602

 

 

Leucobryum bowringii

602

Light-dark aversion test

625

 

 

Metalloproteinase

602

Mice

638

Microbial pigments

645

Migration

602

Migratory fish

652

 

 

Natural food colourants

645

N-n-butylquinoxalin-
2-carboxamide


625

NS1 gene

618

 

 

Olfactory bulbectomy

625

Ornithine-urea cycle

652

Osmotic stress

652

Ovary

595

 

 

Physiology

660

Polyclonal sera

618

Polysaccharides

612

Prokaryotic expression

618

Proliferation

595

 

 

Rhotorula glutinis

645

 

 

Stress

633

 

 

Telomerase

595

Tinospora cordifolia

612

Toxicity

645

TUNEL

638

 

 

Urea

652

 

 

Zeitgeber

660

 

 

 

 

Correspondent author has been indicated by * sign

 

 

 

Indian Journal of Experimental Biology

Vol. 50, September 2012, pp. 595-601

 

 

Review Article

 

 

 

Regulation of telomerase activity in ovarian granulosa cells

Ewa Chronowska

Institute of Animal Science, Department of Biology of Reproduction, Pratelstvi 815, 10400, Prague, Czech Republic

Follicular development is characterized by intensive proliferation and differentiation of granulosa cells. It was reported that during follicular growth granulosa cells arise from the population of stem cells. One of the main evidences for stem characteristics of the cell is the ability to express telomerase an enzyme complex responsible for integrity and stability of chromosome ends (telomeres). It was demonstrated that telomerase activity in granulosa cells is linked to their proliferation and differentiation status and is under the control of growth factors and steroid hormones. In this review current knowledge on the existence and regulation of telomerase activity in granulosa cells has been presented.

 

 

Indian Journal of Experimental Biology

Vol. 50, September 2012, pp. 602-611

 

 

Effect of methanolic and water extract of Leucobryum bowringii Mitt. on growth, migration and invasion of MCF 7 human breast cancer cells in vitro

G S Manoj1, T R Santhosh Kumar2, Saneesh Varghese3 & K Murugan4 *

1,4 Plant Biochemistry and Molecular Biology Laboratory, Department of Botany,

University College, Thiruvananthapuram 695 034, India

2,3 Rajiv Gandhi Center for Biotechnology, Poojapura, Thiruvananthapuram 695 014, India

Received 17 January 2012; revised 22 June 2012

Inhibitory effects of methanol and water extract of L. bowringii. on the adhesion, migration, invasion and matrix metalloproteinase (MMP) activities of MCF 7 human breast cancer cell line are reported. Cells were cultured with 10, 25,
50 オg/mL methanolic or water extract of L. bowringii. Culture medium containing 0.1% DMSO was used as a solvent control. Ultra structural analysis by electron microscopy revealed typical features of apoptosis. A remarkable dose-response parallelism was observed between methanolic extract with growth, migration and invasion of breast cancer cells. Fractionation of methanolic extract by RP-HPLC revealed a pool of phenolic acids. Hoechst 33342 staining assay reveals massive chromatin condensation and subsequent cleavage of structural components of nucleus. The results indicate that methanol extracts inhibit the growth of human breast cancer cells partially through the inhibition of metallo proteinases MMP-2 and MMP-9 activities. Methanolic extract has more anti-metastatic effects in cell based assay than water extract. Clinical application of L. bowringii extract as a bioactive chemopreventive compound may be helpful in limiting breast carcinoma invasion and metastasis.

 

 

Indian Journal of Experimental Biology

Vol. 50, September 2012, pp. 612-617

 

 

Polysaccharide enriched immunomodulatory fractions from
Tinospora cordifolia (Willd) miers ax hook. F. & Thoms

 

Upendra Sharma1, Manju Bala1, Rikki Saini1, Praveen Kumar Verma1, Neeraj Kumar1, Bikram Singh1,*,
Renuka Kulkarni Munshi2 & Supriya Bhalerao2

1Natural Plant Products Division, CSIR-Institute of Himalayan Bioresource Technology (IHBT), Palampur, 176 061, India

2Department of Clinical Pharmacology, TN Medical College & BYL Nair Hospital, Mumbai Central, Mumbai 400 008, India

Received 20 October 2011; revised 5 June 2012

Tinospora cordifolia is used in Ayurveda as "Rasayanas" to improve the immune system and the body resistance against infections. Polysaccharides are the main constituents which are considered to be responsible for immune enhancement. In this study, immunomodulatory activity of three polysaccharide enriched fractions was evaluated using the polymorphonuclear leukocyte function test. Sugar composition was determined by GC-MS analysis of the derivatised fractions. The active polysaccharide fractions mainly constitute glucose, fructose and arabinose as monomer units.

 

 

Indian Journal of Experimental Biology

Vol. 50, September 2012, pp. 618-624

 

 

In vitro expression studies of non structural 1 protein of
Canine Parvo virus 2 by polyclonal antiserum raised against
CPV2-NS1 protein expressed in Escherichia coli as an antigen

G Ravi Kumar, Shikha Saxena, Lovleen Saxena, Uttara Chaturvedi, Lakshman Santra,
Rajiv Kumar, Aditya Prasad Sahoo, Rajmani, Aswani Kumar, G S Desai,
Sudesh Kumar & Ashok K Tiwari*

Molecular Biology Laboratory, Department of Veterinary Biotechnology, Indian
Veterinary Research Institute, Izatnagar, 243 122, India

Received 3 February 2011; revised 25 May 2012

The canine Parvovirus 2, non-structural 1(NS1) is a novel candidate tumor suppressor gene. To confirm the expression of the NS1 in HeLa cells after transfection there was a need to raise antiserum against CPV2- NS1. Therefore, this study was carried out to express and purify the recombinant NS1(rNS1), and characterize the polyclonal serum. CPV2-NS1, complete coding sequence (CDS) was amplified, cloned in pET32a+ and expressed in BL21 (DE3) (pLysS). SDS鳳AGE analysis revealed that the expression of the recombinant protein was maximum when induced with 1.5 mM IPTG. The 6 ラ His tagged fusion protein was purified on Ni-NTA resin under denaturing conditions and confirmed by western blot using CPV2 specific antiserum. The rabbits were immunized with the purified rNS1 to raise anti-NS1 polyclonal antiserum. The polyclonal serum was tested for specificity and used for confirming the expression of NS1 in HeLa transfected with pcDNA.cpv2.ns1 by indirect fluorescent antibody test (IFAT), flow cytometry and western blot. The polyclonal antiserum against NS1 could be very useful to establish functional in vitro assays to explore role of NS1 in cancer therapeutics.

 

 

Indian Journal of Experimental Biology

Vol. 50, September 2012, pp. 625-632

 

 

Antidepressant and anxiolytic-like effects of 4n, a novel 5-HT3 receptor
antagonist using behaviour based rodent models

Baldev Kumar*, Ankur Jindal, Dilip Kumar Pandey, Shvetank Bhatt,
Thangaraj Devadoss &
Radhakrishnan Mahesh

Department of Pharmacy, Birla Institute of Technology & Science (BITS), Pilani 333 031, India

Received 16 March 2012; revised 20 June 2012

The present study was designed to investigate the putative antidepressant and anxiolytic-like effects of N-n-Butylquinoxalin-2-carboxamide (4n), a novel 5-HT3 receptor antagonist, with an optimal log P (2.01) and pA2 value (7.3) greater than ondansetron (6.9) using rodent behavioural models of depression and anxiety. Acute treatment of 4n
(1-4 mg/kg, ip) in mice produced antidepressant-like effect in forced swim test (FST) without affecting the baseline locomotion in actophotometer test in mice. 4n (2-4 mg/kg, ip) treatment also potentiated the 5-hydroxytryptophan (5-HTP) induced head twitch response in mice. Further, 4n (1-4 mg/kg, ip) treatment antagonized reserpine induced hypothermia in rats. Chronic treatment (14 days) with 4n (1-4 mg/kg) and paroxetine (10 mg/kg) significantly attenuated the behavioural anomalies induced by bilateral olfactory bulbectomy in rats in modified open field
paradigm. An anxiogenic-like behaviour was induced by light alone as the stimulus using light-dark aversion test. 4n (2-4 mg/kg, ip) treatment significantly increased no. of transitions between dark and lit area and the time spent in the lit area. In conclusion, these preliminary investigations confirm that 4n exhibited antidepressant and anxiolytic-like effects in rodent models of depression and anxiety.

 

 

Indian Journal of Experimental Biology

Vol. 50, September 2012, pp. 633-637

 

 

Role of C-fibers during acute and chronic stress on formalin-induced
paw edema in rats

Zahra Sepehri1, Masoud Fereidoni1* & Saeed Niazmand2

1Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

2Department of Physiology and cardiovascular research center, Faculty of Medicine,
Mashhad University of Medical Sciences, Mashhad, Iran

Received 2 March 2012; revised 22 June 2012

Stimulation of peripheral nociceptors leads to releasing of some mediators such as substance P (SP) and Calcitonin gene-related peptide (CGRP) and contributes to the edema formation by vasodilatation induction. On the other hand glucocorticoids have anti-inflammatory action, and they are elevated in the plasma during stress. This communication reports C-fibers inflammatory role and the effects of chronic and acute stress and/or dexamethasone (as stress mimicry) on paw edema induced by formalin at presence/deficit C-fibers rats. Acute stress and dexamethasone and chronic dexamethasone have shown an anti-inflammatory effect in C-normal groups, but chronic stress had no effect on inflammation. C-fibers reduction (C-lesion) had anti-inflammatory effects. In deficit C-fibers rats, acute and chronic stress had not stronger anti-inflammatory effect, but acute dexamethasone reduced the anti-inflammatory effect of C-fibers reduction while in the same condition, chronic dexamethasone induced stronger anti-inflammatory effect. The results show C-fiber nerve produce and release the peripheral inflammatory mediators, "C-fibers reduction" decreased the paw inflammation. Counter adaptation in C-lesion animals may reduce the modulatory effects of dexamethasone on the remaining C-fibers. Acute dexamethasone diminished the "C-fibers reduction" anti-inflammatory effect, but at chronic treatment, the modulatory effects of dexamethasone aggregated and it augmented the C-fibers reduction anti-inflammatory effect.

 

 

Indian Journal of Experimental Biology

Vol. 50, September 2012, pp. 638-644

 

 

Protective effects of subchronic caffeine administration on cisplatin induced urogenital toxicity in male mice

M Khazaei1, P D Bayat2, A Ghanbari1*, S Khazaei1, M Feizian3, A Khodaei3 & H A S Alian3

1Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, P.O. Box 1568, Kermanshah, Iran

2Department of Anatomy, Arak University of Medical Sciences, Arak., Iran

3Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran

Received 18 February 2012

In Cisplatin treated group, the degeneration intensity of the kidneys the diameter of seminiferous tubules as well as the apoptotic index in testes and kidney were increased. In Caffeine+Cisplatin treated groups, the total body weight, the weight of testes and kidneys and also the histopathological data did not show significant differences. The motility of sperm in cisplatin group reduced but in Caffeine+Cisplatin groups this parameter was increased. These data suggest that caffeine recovers toxicity induced by cisplatin in both kidneys and testes of mice.

 

 

Indian Journal of Experimental Biology

Vol. 50, September 2012, pp. 645-651

 

 

Thirteen-week oral toxicity study of carotenoid pigment from
Rhodotorula glutinis DFR-PDY in rats

B V Latha1 & K Jeevaratanm2*

1Microbiology Discipline, Defence Food Research Laboratory, Siddarthanagar, Mysore 570 011, India

2Department of Biochemistry and Molecular Biology, Pondicherry University, Kalapet, Puducherry 605 014, India

Received 18 August 2011; revised 3 May 2012

Carotenoids from some of the coloured yeasts like Rhodotorula, Phaffia rhodozyma have attracted commercial interest as a natural pigment for foods. Red yeast isolated from contaminated Potato dextrose agar plate (PDA), designated as Rhodotorula glutinis DFR-PDY has been found to produce carotenoids. In the present study toxicological evaluation of carotenoid pigment has been reported. Experiment was conducted on 3 groups of albino rats. One group with vehicle control (palm oil) and 2 groups with two different doses of red yeast pigment (lower and higher dose) were fed to rats (both male and female) by gavages for 13 weeks. Gain in body weight of rats and food consumption were monitored at regular intervals. Hematological studies revealed that there is no much difference in erythrocytes, packed cell volume, Mean corpuscular volume (MCV), Mean corpuscular haemoglobin concentration (MCHC), platelets and differential counts. Total leucocyte count (TLC) is less in case of higher dose group than the lower and control groups. Whereas, hemoglobin is more in case of higher dose than the lower dose group and least in control group. Even clinico-chemical parameters and urine analysis of vehicle control group and pigment fed rats revealed that there were no major differences between them as well as between two different genders of rats and also interaction between different doses and the genders. Histopathology of these experimental animals revealed that there are no major histological changes found between the groups. It may be concluded that the whole pigment extract from R. glutinis DFR-PDY may be used safely in food preparations as food colourant with an added benefit of antioxidant activity .

 

 

Indian Journal of Experimental Biology

Vol. 50, September 2012, pp. 652-659

 

 

Functional ureogenesis and changes of amino acid metabolism in amphihaline shad hilsa (Tenualosa ilisha, Hamilton-Buchanan) while inhabiting
in estuary and freshwater habitats

 

Umesh C Goswami1, Makibur Rahman1 & Nirmalendu Saha2 *

1Department of Zoology, Gauhati University, Guwahati 781 014, India
2Biochemical Adaptation Laboratory, Department of Zoology, North-Eastern Hill University, Shillong 793 022, India

Received 4 January 2012; revised 8 June 2012

The possible occurrence of a functional ornithine-urea cycle (OUC) and changes of activity of key amino acid metabolism-related enzymes were studied in the amphihaline shad hilsa (Tenualosa ilisha) that were collected from estuarine water of Kakdwip and from freshwater river basin of Bhrahmaputra during the breeding season. Very high concentration of urea was detected in different tissues and plasma of shad hilsa collected from estuarine water compared to the one collected from freshwater river basin. This observation clearly suggests that the shad hilsa has the potential of synthesizing and retaining urea inside the body for the purpose of osmoregulation while living in hypertonic saline environment of estuary. This was accompanied by the presence of high activity of all the five OUC enzymes in hepatic and in certain non-hepatic tissues such as the kidney and muscle of shad hilsa in support of its potential ureogenic capacity while inhabiting in estuarine water. The activities of different key amino acid metabolism-related enzymes such as glutamine synthetase, glutamate dehydrogenase, alanine aminotransaminase and aspartate aminotransaminase were also found to be significantly higher in shad hilsa of estuarine water compared to the one collected from freshwater habitat. Thus the adjustment to amino acid metabolism in shad hilsa in different environmental salinities appears to play significant roles for osmotic balance and also for proper energy supply in addition to the presence of a functional OUC while migrating between marine and freshwater habitats throughout their life cycle.

 

 

Indian Journal of Experimental Biology

Vol. 50, September 2012, pp. 660-664

 

 

Eclosion behaviour of three species of Drosophila under different light regimes

Shereen Kouser & V Shakunthala*

Drosophila Stock Centre, Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore 570 006, India

Received 1 August 2011; revised 3 February 2012

The daily patterning of animal behaviour is presumed to be due to changes in responsiveness to external stimuli, as a function of varying internal states which may be oscillating with a 24 h period. The endogenous circadian system functions to organize behaviour and physiology to adapt to and anticipate environmental changes in light, temperature, food and mate availability. Light is most important zeitgeber that influence circadian physiology of several insects. The present study is an attempt to understand the eclosion behaviour of Drosophila melanogaster (Oregon-K strain), Drosophila gangotrii and Drosophila jambulina under light/dark (LD), continuous light (LL), continuous dark (DD) conditions for 30 generations. The stocks were maintained at 20ア1 C with 75% RH. Eclosion behaviour was analyzed at 4 h intervals until complete emergence of flies. Result reveals that in all the species peak of eclosion was found at dawn and on subjective day. Intraspecies comparison shows that there was no significant difference in eclosion at different time point and generations under different light regimes. However, interspecies comparison shows significant difference for hours. The data from experiments showed altered rhythm in the form of phase advance and phase delay in eclosion at different light regimes suggesting the role of photoperiod in the physiology of organisms.