Indian Journal of Experimental Biology

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VOLUME 51

NUMBER 3

MARCH 2013

CODEN: IJEB (A6) 51 (2) 181-268 (2013)

ISSN: 0019-5189 (Print); 0975-1009 (Online)

 

CONTENTS

 

 

Review Article

 

 

 

Cell phone radiation exposure on brain and associated biological systems

187

 

 

Kavindra Kumar Kesari, Mohd. Haris Siddiqui, Ramovatar Meena, H N Verma & Shivendra Kumar

 

 

 

Papers

 

 

 

Sonic hedgehog inhibition induces mouse embryonic stem cells to differentiate toward definitive endoderm

201

 

 

Ali Ghanbari, Mozafar Khazaei, Mahmoud Hashemi-Tabar, Arezou Rabzia, Fardin Fathi & Parvin- Dokht Bayat

 

 

 

Expression of JMJD2A in infiltrating duct carcinoma was markedly higher than fibroadenoma, and associated with expression of ARHI, p53 and ER in infiltrating duct carcinoma

208

 

 

Bei-Xu Li, Jia Li, Cheng-Liang Luo, Ming-Chang Zhang, Hui Li, Li-Liang Li, Hong-Fei Xu, Yi-Wen Shen, Ai-Min Xue & Zi-Qin Zhao

 

 

 

Regulation of luteinizing hormone receptor in hippocampal neurons following different long-lasting treatments of castrated adult rats

218

 

 

H Abtahi, M Shabani, S B Jameie, A H Zarnani, S Talebi, N Lakpour , H Heidari-Vala, H Edalatkhah, M.A Akhondi, M Amiri, A R Mahmoudi & M R Sadeghi

 

 

 

Intravenous human umbilical cord blood improves elctrophysiological and metabolic properties in ISO induced myocardial necrosis in rats

228

 

 

Mahalaxmi Mohan, Rahul Rokade & Sanjay Kasture

 

 

 

Effect of Naja naja Laurenti shed skin extract on estrous cycle, hormone -cytokines profiles, histopathology of ovary and uterus of Swiss albino mice

235

 

 

Sanghamitra Mukherjee, Subir Chandra Dasgupta & Antony Gomes

 

 

 

Protective effect of eicosapentaenoic acid-docosahexaenoic acid and α僕inolenic acid rich phytosterol ester on brain antioxidant status and brain lipid composition in hypercholesterolemic rats

241

 

 

Avery Sengupta & Mahua Ghosh

 

 

Effects of fluoride and ethanol administration on lipid peroxidation systems in the rat brain

249

 

 

Shailender Singh Chauhan, Sudarshan Ojha & Akhtar Mahmood

 

 

 

Protective effect of lawsone on L-Arginine induced acute pancreatitis in rats

256

 

 

Sandeep Biradar & B Veeresh

 

 

 

Biodiesel production from marine cyanobacteria cultured in plate and tubular photobioreactors

262

 

 

B Karpanai Selvan, M Revathi, P Sobana Piriya, P Thirumalai Vasan, D Immuanual Gilwax Prabhu & S John Vennison

 

 

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Editor痴 Note

The Indian Journal of Experimental Biology is covered by the following international abstracting and indexing services:

 

Science Citation Index ExpandedTM

PubMed (http://www.ncbi.nim.nih.gov/)

MEDLINE

BIOSIS

Chemical Abstracts Service

Excerpta Medica

Informascience

Refrativnyi Zhurnal

Zoological Records

 

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Indian Journal of Experimental Biology in Open Access Mode

 

The Indian Journal of Experimental Biology (IJEB) is now an open access journal in the repository, NISCAIR Online Periodicals Repository (NOPR) [http://nopr.niscair.res.in].

Full text of all articles published in IJEB from 2006 onwards can now be accessed at NOPR in the open access mode. Papers in the current issue shall be uploaded immediately. Papers published in earlier years shall be added soon.

NOPR is based on DSpace, a digital repository software, and allows document browsing, document searching and various search options like title, author name, keywords, year, issue, etc.

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Author Index

Abtahi H

218

Akhondi M.A

218

Amiri M

218

 

 

Bayat Parvin-Dokht

201

Biradar Sandeep

256

 

 

Chauhan Shailender Singh

249

 

 

Dasgupta Subir Chandra

235

 

 

Edalatkhah H

218

 

 

Fathi Fardin

201

 

 

Ghanbari Ali

201

Ghosh Mahua

241

Gomes Antony

235

 

 

Heidari-Vala H

218

 

 

Jameie S B

218

 

 

Karpanai Selvan B

262

Kasture Sanjay

228

Kesari Kavindra Kumar

187

Khazaei Mozafar

201

 

 

Lakpour N

218

Li Bei-Xu

208

Li Hui

208

Li Jia

208

Li Li-Liang

208

Luo Cheng-Liang

208

 

 

Mahmood Akhtar

249

Mahmoudi A R

218

Meena Ramovatar

187

Mohan Mahalaxmi

228

Mukherjee Sanghamitra

235

 

 

Ojha Sudarshan

249

 

 

Piriya P Sobana

262

Prabhu D Immuanual Gilwax

262

 

 

Rabzia Arezou

201

Revathi M

262

Rokade Rahul

228

 

 

Sadeghi M R

218

Sengupta Avery

241

Shabani M

218

Shen Yi-Wen

208

Shivendra Kumar

187

Siddiqui Mohd. Haris

187

 

 

Tabar Mahmoud Hashemi-

201

Talebi S

218

 

 

Vasan P Thirumalai

262

Veeresh B

256

Vennison S John

262

Verma H N

187

 

 

Xu Hong-Fei

208

Xue Ai-Min

208

 

 

Zarnani A H

218

Zhang Ming-Chang

208

Zhao Zi-Qin

208

 

Keyword Index

Acute pancreatitis

256

Alpha-Linolenic acid

241

Anti-inflammatory

256

Antioxidant enzymes

228,241

 

 

Biodiesel

262

 

 

Cancer

187

Cardiac marker enzymes

228

Co-exposure

249

Correlation

208

Cytokines

256

 

 

Definitive endoderm

201

Dithizone

201

Docosahexaenoic acid

241

 

 

ECG

228

Eicosapentaenoic acid

241

Endocrine profile

235

Estrous cycle

235

Ethanol

249

 

 

Fibroadenoma

208

Fluoride

249

 

 

Hedgehog interacting protein

201

Hippocampus

218

hUCBC

228

Hypercholesterolemia

241

 

 

Immature isoform

218

Infiltrating duct carcinoma

208

Isoproterenol

228

 

 

JMJD2A

208

 

 

L-arginine

256

Lawsone

256

LED photobioreactor

262

LHR

218

Lipid

262

Lipid peroxidation

249

Lipid profile

241

Luteinizing hormone

218

Lyngbya sp.

262

 

 

Mesendoderm

201

Mobile phone

187

Mouse embryonic stem cell

201

 

 

Naja naja

235

Neurotransmitter amino acids

241

 

 

Rat brain

249

Reactive oxygen species

187

Regulation

218

 

 

Salinity

262

Shed snake skin

235

Steroids

218

Sterol esters

241

Synechococcus sp.

262

 

 

Tumor formation

187

Tumor related protein

208

 

Correspondent author is marked by *

 

 

Indian Journal of Experimental Biology

Vol.51, March 2013, pp. 187-200

 

 

 

Review Article

 

 

 

Cell phone radiation exposure on brain and associated biological systems

 

Kavindra Kumar Kesari1,3*, Mohd. Haris Siddiqui2, Ramovatar Meena3, H N Verma1 & Shivendra Kumar4

1School of Life Sciences, Jaipur National University, Jaipur 302 001, India

2Department of Bioengineering, Faculty of Engineering, Integral University, Lucknow, India

3Bioelectromagnetic Laboratory, School of Environmental Sciences
Jawaharlal Nehru University, New Delhi 110 067, India

4International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India

Wireless technologies are ubiquitous today and the mobile phones are one of the prodigious output of this technology. Although the familiarization and dependency of mobile phones is growing at an alarming pace, the biological effects due to the exposure of radiations have become a subject of intense debate. The present evidence on mobile phone radiation exposure is based on scientific research and public policy initiative to give an overview of what is known of biological effects that occur at radiofrequency (RF)/ electromagnetic fields (EMFs) exposure. The conflict in conclusions is mainly because of difficulty in controlling the affecting parameters. Biological effects are dependent not only on the distance and size of the object (with respect to the object) but also on the environmental parameters. Health endpoints reported to be associated with RF include childhood leukemia, brain tumors, genotoxic effects, neurological effects and neurodegenerative diseases, immune system deregulation, allergic and inflammatory responses, infertility and some cardiovascular effects. Most of the reports conclude a reasonable suspicion of mobile phone risk that exists based on clear evidence of bio-effects which with prolonged exposures may reasonably be presumed to result in health impacts. The present study summarizes the public issue based on mobile phone radiation exposure and their biological effects. This review concludes that the regular and long term use of microwave devices (mobile phone, microwave oven) at domestic level can have negative impact upon biological system especially on brain. It also suggests that increased reactive oxygen species (ROS) play an important role by enhancing the effect of microwave radiations which may cause neurodegenerative diseases.

 

 

Indian Journal of Experimental Biology

Vol.51, March 2013, pp. 201-207

 

 

Sonic hedgehog inhibition induces mouse embryonic stem cells to differentiate toward definitive endoderm

Ali Ghanbari1, Mozafar Khazaei1,*, Mahmoud Hashemi-Tabar2, Arezou Rabzia1, Fardin Fathi3 & Parvin-Dokht Bayat4

1Fertility and Infertility Research Center, Kermanshah University of Medical Sciences,
Kermanshah,
P.O. Box 1568, Iran, 6714869914

2Cell and Molecular Research Center, Faculty of Medicine, Ahwaz University of Medical Sciences, Ahwaz, Iran

3Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran

4Department of Anatomy, Arak University of Medical Sciences, Arak, Iran

Received 8 August 2012; revised 29 October 2012

In the experimental group (shh inhibited group), there were significant decreases in the expression of Oct4, Nanog, Shh, GATA4, Brachyury and Goosecoid, while increases were observed for TAT and Pdx1. The expression of Sox17 did not differ between two control and experimental groups. In experimental group, the amount of GSC positive cells was somehow lower but it seems that there was no difference for Sox17. Shh inhibition induces ESCs to differentiate toward definitive endoderm by committing mesendodermal lineages.

 

 

Indian Journal of Experimental Biology

Vol.51, March 2013, pp. 208-217

 

 

Expression of JMJD2A in infiltrating duct carcinoma was markedly higher
than fibroadenoma, and associated with expression of ARHI, p53 and ER
in infiltrating duct carcinoma

Bei-Xu Li, Jia Li, Cheng-Liang Luo, Ming-Chang Zhang, Hui Li, Li-Liang Li, Hong-Fei Xu,
Yi-Wen Shen, Ai-Min Xue & Zi-Qin Zhao*

Department of Forensic Medicine, Shanghai Medical College,
Fudan University, 138 Yixueyuan Road, Shanghai 200032, PR China

Received 25 June 2012; revised 12 October 2012

Jumonji Domain Containing 2A (JMJD2A) may be a cancer-associated gene involved in human breast cancer. With a view to investigating expression of JMJD2A in human breast cancer and benign lesion tissues as well as relationship between JMJD2A and tumor related proteins, histological and immunohistochemical analysis, Western blot and quantitative real-time PCR in infiltrating duct carcinoma and fibroadenoma for JMJD2A and immunohistochemical analysis and quantitative real-time PCR in infiltrating duct carcinoma for tumor related proteins (ARHI, p53, ER, PR and CerbB-2) were performed. Histological examination validated the clinical diagnosis. The JMJD2A positive rate of infiltrating duct carcinoma was significantly higher than fibroadenoma by immunohistochemical analysis. The mean optical density of JMJD2A in infiltrating duct carcinoma was higher than fibroadenoma by western blot. JMJD2A mRNA level in infiltrating duct carcinoma was higher than fibroadenoma by quantitative real-time PCR. Spearman correlation analysis revealed that the expression of JMJD2A was associated with ARHI, p53 and ER from immunohistochemical results respectively. Pearson correlation analysis revealed that the expression of JMJD2A was associated with ARHI, p53 and ER from quantitative real-time PCR results respectively. Expression of JMJD2A in infiltrating duct carcinoma was higher, and associated with ARHI, p53 and ER. The results may take JMJD2A as a potential diagnostic and therapeutic target in human breast cancer.

 

 

Indian Journal of Experimental Biology

Vol.51, March 2013, pp. 218-227

 

 

Regulation of luteinizing hormone receptor in hippocampal neurons following different long-lasting treatments of castrated adult rats

H Abtahi1, M Shabani2, S B Jameie3, A H Zarnani4,5, S Talebi6, N Lakpour4, H Heidari-Vala7, H Edalatkhah7,
M.A Akhondi7, M Amiri1, A R Mahmoudi6 & M R Sadeghi6,*

1Department of Basic sciences, Faculty of Allied Medicine, Gonabad University of Medical Sciences, Gonabad, Iran

2Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran

3Department of Basic Sciences, Faculty of Allied Medicine & Cellular and Molecular Research Center,
Tehran University of Medical Sciences, Tehran, Iran

4Nanobiotechnology Research Center, Avicenna Research Institute,
Academic Center for Education Culture and Research (ACECR), Tehran, Iran

5Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran

6Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

7Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Received 4 July 2012; revised 27 November 2012

The aim of this study was to investigate the effects of different Luteinizing hormone (LH) and steroid hormones levels on LH receptor (LHR) expression in the hippocampal cells. Rats (24 males and 24 females) were assigned to four groups: one control and three experimental [gonadectomy (GDX), gonadectomy + gonadotropin releasing hormone analogue (GDX+GnRHa) and GDX+GnRHa+estradiol (E2) or testosterone (T)] independently for each gender. All experimental rats were gonadectomized; then GnRHa was administrated to GDX+GnRHa group, and GnRHa plus steroid hormone to GDX+GnRHa+E2 or T group in both genders for four-month. LHR mRNA expression and its protein level in hippocampal cells were measured using QRT-PCR and Western blotting. Quantification of mRNA revealed a decrease in LHR transcripts level in GDX+GnRHa group of females. A significant change was observed between GDX groups and GDX+GnRHa+E2 or T versus GDX+GnRHa group in females. High levels of LH decreased significantly the immature isoform of LHR in GDX group compared to control group in both genders, but low LH concentrations in GDX+GnRHa group induced immature LHR isoform production only in females. Therefore increased LH concentration induces production of incomplete LHR transcripts in hippocampal cells and decreases immature LHR at the protein level. This implies that LH decreases the efficiency of translation through either producing non-functional LHR molecules or preventing their translation.

 

 

Indian Journal of Experimental Biology

Vol.51, March 2013, pp. 228-234

 

 

Intravenous human umbilical cord blood improves elctrophysiological and metabolic properties in ISO induced myocardial necrosis in rats

 

Mahalaxmi Mohan*, Rahul Rokade & Sanjay Kasture

Department of Pharmacology, M. G. V痴 Pharmacy College, Panchavati, Nashik, Maharashtra 422 003, India
Sanjivani College of Pharmaceutical Education Research, Kopargaon, Ahmednagar 423 603, India

Received 18 February 2012; revised 14 August 2012

Rats treated with isoproterenol (ISO, 85 mg/kg, sc, twice at an interval of 24 h) showed a significant increase in heart rate, mean arterial blood pressure, pressure rate index, ST elevation on ECG, and a significant increase in the levels of cardiac marker enzymes- lactate dehydrogenase, and creatine kinase in serum and a significant reduction in superoxide dismutase, and catalase and increase in thiobarbituric acid reactive substance activity in heart tissue. Treatment with Human umbilical cord blood (hUCBC; 500 and 1000 オL, iv, via the tail vein; 2 h after the second dose of ISO) significantly restored back to normal levels and showed a lesser degree of cellular infiltration and infarct size in histopathological and planimetry studies respectively. Thus, hUCBC ameliorates cardiotoxic effects of isoproterenol and may be of value in the treatment of myocardial infarction.

 

 

Indian Journal of Experimental Biology

Vol.51, March 2013, pp. 235-240

 

 

Effect of Naja naja Laurenti shed skin extract on estrous cycle, hormone - cytokine profiles, histopathology of ovary and uterus of Swiss albino mice

Sanghamitra Mukherjee1, Subir Chandra Dasgupta1,* & Antony Gomes2

1Post Graduate Department of Zoology, Maulana Azad College, 8 Rafi Ahmed Kidwai Road, Kolkata 700 032, India

2Laboratory of Toxinology & Experimental Pharmacodynamics, Department of Physiology, University of Calcutta

92, A P C Road, Kolkata 700 009, India

Received 23 July 2012; revised 18 December 2012

The snake shed skin though considered as biological waste products have been mentioned in folk and traditional medicine for treatment of ailments like skin disorders, parturition problems etc. Shedded skin extract (5 mg.kg-1, sc) did not produce any change in the estrous cycle of normal cycling female mice. However in 10 mg.kg-1, sc dose, the extract caused a temporary cessation of the estrous cycle at diestrous phase in normal cycling female mice for 10 days. SSAE (10 mg.kg-1, sc) caused a significant change in the level of LH, FSH, progesterone, estradiol, IL-1β, IL-6 and TNF-α. Histopathology of uterus and ovary showed structural disorientation in both. The results substantiate the influence of snake shed skin in mice reproductive cycle.

 

 

Indian Journal of Experimental Biology

Vol.51, March 2013, pp. 241-248

 

 

Protective effect of eicosapentaenoic acid-docosahexaenoic acid and
α僕inolenic acid rich phytosterol ester on brain antioxidant status and brain
lipid composition in hypercholesterolemic rats

Avery Sengupta* & Mahua Ghosh

Department of Chemical Technology,University of Calcutta,
92, A.P.C. Road, Kolkata 700 009, India

Received 18 April 2012; revised 10 December 2012

With a view to investigate the ameliorative effects of sitosterol esters against degenerative effects of hypercholesterolemia brain antioxidant enzyme assays, brain lipid profile, brain phospholipid compositional change and brain neurotransmitter concentrates (glutamic acid, asparctic acid, glycine) were measured in hypercholesterolemic rats. The results indicated that phytosterol esters have a role in countering hypercholesterolemia-related changes in the brain by decreasing the cholesterol levels, increasing the phospholipid levels and increasing the level of antioxidant enzymes. The results suggest that phytosterol esters may be of therapeutic significance and may offer new and effective options for the treatment of hypercholesterolemia-induced changes in the brain.

 

 

Indian Journal of Experimental Biology

Vol.51, March 2013, pp. 249-255

 

 

Effects of fluoride and ethanol administration on lipid peroxidation systems
in rat brain

Shailender Singh Chauhan, Sudarshan Ojha & Akhtar Mahmood*

1Department of Biochemistry, Panjab University, Chandigarh, 160 014, India

Received 6 June 2012; revised 21 September 2012

Exposure to fluoride and excessive ethanol consumption has been identified as a serious public health problem in many parts of the world, including India. Thus, the effect of co-exposure to fluoride and ethanol for 3-6 weeks was studied on lipid peroxidation (LPO) and oxidative stress related parameters in the rat brain. After 3 weeks, co-treated animals showed 95% increase in LPO levels compared to control. However, the levels of reduced glutathione, total and protein thiols were decreased. These changes were accompanied by a decrease in the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase. Rats exposed to fluoride together with ethanol for 6 weeks resulted in 130% increase in LPO and decrease in the reduced glutathione levels. The activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase were reduced under these conditions. Brain histology revealed excessive lymphocytes, edema and spongeosis in the cortical region after six weeks of fluoride and ethanol treatment. These results suggest that exposure to fluoride together with ethanol enhances lipid peroxidation by affecting antioxidant defence systems in the rat brain.

 

 

Indian Journal of Experimental Biology

Vol.51, March 2013, pp. 256-261

 

 

Protective effect of lawsone on L-Arginine induced acute pancreatitis in rats

 

Sandeep Biradar* & B Veeresh

Department of Pharmacology, G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad 500 028, India

Received 20 June 2012; revised 11 December 2012

The efficacy of lawsone against L-arginine induced acute pancreatitis was determined at 24 h by determination of serum levels of amylase, lipase and proinflammatory cytokines [tumor necrosis factor (TNF)-α, C-reactive proteins and interleukin (IL)], pancreatic myeloperoxidase (MPO) activity, lipid peroxidation (thiobarbituric acid reactive substances (TBARS)], nitrate/nitrite levels, and the wet weight/body weight ratio. Lawsone and methylprednisolone treatments significantly attenuated the L-arginine- induced increases in pancreatic wet weight/body weight ratio, and decreased the serum levels of amylase and lipase, and TNF-α and IL-6 and significantly lowered pancreatic levels of MPO, TBARS, and nitrate/nitrite. The histoimmunological findings further proved the amelioration of pancreatic injury by lawsone and further proved anti-inflammatory and antioxidant agent property of lawsone.

 

 

Indian Journal of Experimental Biology

Vol.51, March 2013, pp. 262-268

 

 

Biodiesel production from marine cyanobacteria cultured in plate and
tubular photobioreactors

B Karpanai Selvan, M Revathi, P Sobana Piriya, P Thirumalai Vasan,

D Immuanual Gilwax Prabhu & S John Vennison*

Department of Biotechnology, Anna University-BIT Campus, Tiruchirappalli 620 024, India

Received 28 October 2011; revised 12 November 2012

Carbon (neutral) based renewable liquid biofuels are alternative to petroleum derived transport fuels that contribute to global warming and are of a limited availability. Microalgae based biofuels are considered as promising source of energy. Lyngbya sp. and Synechococcus sp. were studied for the possibility of biodiesel production in different media such as ASNIII, sea water enrichment medium and BG11. The sea water enrichment medium was found superior in enhancing the growth rate of these microalgae. Nitrogen depletion has less effect in total chlorophyll a content, at the same time the lipid content was increased in both Lyngbya sp. and Synechococcus sp. by 1.4 and 1.2 % respectively. Increase in salinity from 0.5-1.0 M also showed an increase in the lipid content to 2.0 and 0.8 % in these strains; but a salinity of 1.5 M has a total inhibitory effect in the growth. The total biomass yield was comparatively higher in tubular LED photobioreactor than the fluorescent flat plated photobioreactor. Lipid extraction was obtained maximum at 60 コC in 1:10 sample: solvent ratio. GC-MS analysis of biodiesel showed high content of polyunsaturated fatty acids (PUFA; 4.86 %) than saturated fatty acid (SFA; 4.10 %). Biodiesel production was found maximum in Synechococcus sp. than Lyngbya sp. The viscosity of the biodiesel was closely related to conventional diesel. The results strongly suggest that marine microalgae could be used as a renewable energy source for biodiesel production.