Indian Journal of Experimental Biology

ISSN : 0019-5189

CODEN : IJEB (A6) 41(4) 279-382 (2003)

VOLUME 41

NUMBER 4

APRIL 2003

 

CONTENTS

 

Papers

 

Chronic administration of caffeine: Effect on the activities of hepatic antioxidant enzymes of Ehrlich ascites tumor-bearing mice

Sutapa Mukhopadhyay (Kali), Anup Mondal & Mrinal K Poddar

 

283

 

 

Effect of ascorbic acid on stimulatory status of activated mouse peritoneal phagocytes

Rachna Agarwal, A K Tripathi & Asit K Chakrabarty

290

 

 

Mechanism of action of antiatherogenic and related effects of ficus bengalensis Linn. flavonoids in experimental animals

Regi Susan Daniel, K s Devi, K T Augusti & C R Sudhakaran Nair

 

296

 

 

Evaluation of Pongamia pinnata root extract on gastric ulcers and mucosal offensive and defensive factors in rats

T Prabha, M Dora Babu, S Priyambada, V K Agrawal & R K Goel

 

304

 

 

Effect of ethanol extract of Piper betle Linn leaf on healing of NSAID-induced experimental ulcer  A novel role of free radical scavenging action

Biswajit Majumdar, Susri Guha Ray Chaudhuri, Arun Ray & Sandip K Bandyopadhyay

 

311

 

 

Preliminary studies on acute and subacute toxicity of an antidiabetic herbal preparation, Dianex

S Mutalik, B Sulochana, M Chetana, N Udupa & P Uma Devi

 

316

 

 

Characterisation of fowl adenoviruses from chickens affected with infectious

hydropericardium during 1994-1998 in India

Samadhan J Jadhao, J N Deepak, J M Kataria, R S Kataria, A K Tiwari, R Somvanshi, P Sangamithra & K C Verma

 

321

 

 

Heterochrony in cotton bollworm, Earias vitella (Fb) following ethoxyprecocene administration

Irfan Ahmad Khan & Krishna Kumar

 

328

 

 

Toxicity of PCB 1232 on mitochondria of fish Arius caelatus (Valenciennes)

Dravyam Selvarani & C Rajamanickam

336

 

 

Associative diazotrophs of pearl millet (Pennisetum glaucum) from semi arid region  Isolation and characterization

Mala Tiwari, Shashi Paroda & K R Dadarwal

 

341

 

 

Generation and screening of recombinant inbred lines of rice for yellow stemborer resistance

S Mohankumar, V Thiruvengadam, K Samiayyan & P Shanmugasundaram

 

346

 

 

Modulation by phenolic compounds of ABA-induced inhibition of mustard (Brassica juncea L. cv. RLM 198) seed germination

Shashi Sharma, Shanti S Sharma & Vinay K Rai

 

352

 

 

Notes

 

Possible role of granulocyte macrophage colony stimulating factor receptor

(GM-CSF R) in malaria

Ananth N, Beena V Shetty  &  D M Vasudevan

 

357

 

 

In vitro efficacy of ciprofloxacin alone and in combination with amoxycillin against Salmonella typhi isolates

S Mandal, Manisha (Deb) Mandal & N K Pal

 

360

 

 

Antidiarrhoeal effects of methanolic root extract of Hemidesmus indicus

(Indian sarsaparilla)  An in vitro and in vivo study

Sarita Das, R Prakash & S Niranjali Devaraj

 

363

 

 

Protection of adrenocortical activity by dietary casein in ether anaesthetized rats

N M Biswas, A Chattopadhyay & M Sarkar

367

 

 

Effect of sodium glycocholate on development of tolerance to Parthenium hysterophorus extracts

Arshia Shariff, Sarasija Suresh, K L K Paranjothy & K V Nagendra Prasad

 

370

 

 

Amylase induction during seed germination in isoproturon susceptible and resistant biotypes of Phalaris minor Retz.

R S Dhawan, A K Dhawan, S Kajla & R Moudgil

 

373

 

 

Evaluation of entomopathogenic nematode, Steinernema feltiae against field population of mustard sawfly, Athalia lugens proxima (Klug) on radish

K Narayanan & C Gopalakrishnan

 

376

 

 

Modified behaviour in nucleopolyhedro virus infected field bean pod borer, Adisura atkinsoni and its impact on assessing the field efficacy of NPV

K Narayanan

 

379

   
Author Index
 

 

Announcement

 

Hands-on Workshop on Laboratory Methods in Cellular
& Molecular Biology

1017 November 2003, New Delhi

With an aim to impart an in-depth practical and wet-lab experience on a wide range of cellular and molecular biology techniques, a workshop will be held at the Department of Physiology, AIIMS, New Delhi for a limited number of students (1012) from all possible parts of India. The techniques covered will be: (a) Cell Culture, (b) Confocal Microscopy, (c) c-DNA Expression Arrays, (d) Immunohistochemistry, (e) In situ Hybridization, (f) Northern Blot, and (g) Real Time PCR.

Interested PhD and post-doctoral students may send their applications, duly forwarded by their Head of the Department or supervisor, along with their bio-data before 30 August 2003 to The Organising Secretariat, Workshop on Laboratory Methods in Cellular & Molecular Biology, Department of Physiology, All India Institute of Medical Sciences, New Delhi 110 029. Budget type accommodation may be provided to the outstation candidates.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 283-289

 

Papers  

 

Chronic administration of caffeine: Effect on the activities of hepatic antioxidant enzymes of Ehrlich ascites tumor-bearing mice

Sutapa Mukhopadhyay (Kali), Anup Mondal & Mrinal K Poddar*

 

Received 17 June 2002; revised 13 February 2003

Chronic ingestion (for 22-30 consecutive days) of caffeine (20 mg/kg/day, po) increased the activities of the hepatic enzymes- catalase (CAT) and superoxide dismutase (SOD) and decreased its lipid peroxidation (LP) in mice. Development of Ehrlich ascites carcinoma (EAC) cell decreased the activities of hepatic CAT and SOD and increased LP. But pretreatment of caffeine for 12 consecutive days and continuation of its treatment during the course of development of EAC cells restored the EAC cell-induced changes in liver CAT, SOD and LP to their corresponding control values. Thus, the present results by confirming the results of others previously published, suggest that caffeine is an antioxidant and may act as an anticarcinogen.

 

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 290-295

 

 

Effect of ascorbic acid on stimulatory status of activated
mouse peritoneal phagocytes

Rachna Agarwal, A K Tripathi & Asit K Chakrabarty

 

Received 24 September 2002; revised 21 January 2003

Mouse peritoneal macrophages (MPM) when elicited by the antioxidant ascorbic acid have been found to be significantly stimulatory, exhibiting marked alteration at the cellular and enzyme levels. Alterations recorded were as follows - cellular yield per mouse, their protein content, lysosomal acid hydrolase levels and capability to phagocyte, all were significantly enhanced. The new stimulant was observed to produce no synergistic action on MPM when thioglycollate, BCG or endotoxin along with the same stimulated the latter. Levels of antioxidants like ascorbic acid and glutathione were found to be enhanced in elicited macrophages whereas superoxide dismutase levels varied when the three above stimulators were administered. However, the ascorbic acid elicited cells showed an increase in glutathione levels and a decrease in SOD levels but no change in total intracellular ascorbic acid levels. Further, though ascorbic acid interaction enhanced the phagocytic capability of MPM as compared to resident cells, no significant boosting of phagocytic process could be observed when each of three stimulators coupled with ascorbic acid was used for macrophage elicitation.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 296-303

 

 

Mechanism of action of antiatherogenic and related effects of
ficus bengalensis Linn. flavonoids in experimental animals

Regi Susan Daniel, K s Devi & K T Augusti

and

C R Sudhakaran Nair

 

Received 26 June 2002; revised 20 February 2003

One month treatment of alloxan diabetic dogs with a glycoside, viz. leucopelargonin derivative (100 mg/kg/day) isolated from the bark of F. bengalensis decreased fasting blood sugar and glycosylated haemoglobin by 34% and 28% respectively. Body weight was maintained in both the treated groups while the same was decreased significantly by 10% in the control group. In cholesterol diet fed rats, as the atherogenic index and the hepatic bile acid level and the faecal excretion of bile acids and neutral sterols increased, the HMGCoA reductase and lipogenic enzyme activities in liver and lipoprotien lipase activity in heart and adipose tissue and plasma LCAT activity and the incorporation of labelled acetate into free and ester cholesterol in liver decreased significantly. On treatment with the two ficus flavonoids, viz. leucopelargonin and leucocyanin derivatives and another flavonoid quercetin (100 mg/kg/day)the above said effects except on bile acids and sterols and lipogenic enzymes were significantly reversed in the cholesterol fed rats. However in the treated rats the hepatic level of bile acids and the faecal excretion of bile acids and neutral sterols still further increased and the action of lipogenic enzyme glucose 6 phosphate dehydrogenase was still further decreased. These effects of leucopelargonidin and quercetin were better than that of the second. Toxicity studies are required to be carried out to find out if the ficus flavonoids could be used as health promoters as they are hypocholesterolemic and antioxidant in action.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 304-310

 

 

Evaluation of Pongamia pinnata root extract on gastric ulcers and mucosal offensive and defensive factors in rats

T Prabha, M Dora Babu, S Priyambada, V K Agrawal & R K Goel

 

Received 15 January 2003; revised 27 February 2003

Effect of methanolic extract of P. Pinnata roots (PPRM) was studied against various experimental gastric ulcer models and offensive and defensive gastric mucosal factors in rats. An initial dose-response study using 12.5-50mg/kg P. Pinnata root extract, when given orally in two divided dose for 4 days + 5th full dose on the day of experiment 60 min before the experiment, indicated 25mg/kg as an optimal regimen and was used for further study. PPRM showed significant protection against aspirin and 4 hr PL, but not against ethanol-induced gastric ulceration. It showed tendency to decrease acetic acid-induced ulcer after 10 days treatment. Ulcer protective effect of PPRM was due to augmentation of mucosal defensive factors like mucin secretion, life span of mucosal cells, mucosal cell glycoproteins, cell proliferation and prevention of lipid per oxidation rather than on the offensive acid-pepsin secretion.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 311-315

 

 

Effect of ethanol extract of Piper betle Linn leaf on healing of NSAID - induced experimental ulcer  A novel role of free radical scavenging action

Biswajit Majumdar, Susri Guha Ray Chaudhuri, Arun Ray & Sandip K Bandyopadhyay

 

Received 16 May 2002; revised 27 January 2003

Treatment with ethanol extract of leaf of P. betle at a dose of 150 mg/kg body weight daily for 10 days, after induction of peptic ulcer by NSAID in albino rats, produced significant healing effect. During healing process, on treatment with the extractive, antioxidative factor, e.g. superoxide dismutase and catalase activity, mucus and total gastric tissue sulfhydryl group were increased. In contrast, oxidised lipid and oxidatively modified proteins were reduced to near normalcy, within 7 to 10 days, however, change in the untreated group was not significant. The extract also showed significant in vitro free radical scavenging action. The results suggest that the antioxidant or free radical scavenging activity of the plant extract, may be responsible for its healing action.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 316-320

 

 

Preliminary studies on acute and subacute toxicity of an antidiabetic
herbal preparation, Dianex

S Mutalik, B Sulochana, M Chetana, N Udupa & P Uma Devi

 

Received 3 December 2002; revised 11 February 2003

Dianex, a polyherbal formulation intended to use for diabetic patients, has been screened for toxic effects. For acute toxicity studies, Dianex was administered orally in graded doses of 0.75-10 g/kg to the mice. For subacute toxicity studies, different doses of Dianex (1.0, 1.5 and 2.5 g/kg) were administered orally to the rats once daily for 30 days. Animals were observed for physiological and behavioural responses, mortality, food and water intake and body weight changes. Hematological evaluation was performed weekly. All the animals were sacrificed on 31st day and changes in organ weights and histology were examined. Biochemical studies were done in liver and serum. No mortality was observed up to 10 g/kg of Dianex in acute toxicity study. Daily administration of as high as 2.5 g/kg dose of Dianex did not result in any mortality or changes in gross behaviour, body weight, weight and histology of different organs or serum and liver biochemistry. However, significant increase in RBC count and hemoglobin level was observed in the treated animals at all doses. Other peripheral blood constituents were in the normal range. The dose of Dianex to produce significant antidiabetic activity in mouse, 0.25-0.5 g/kg, is much lower than the doses used in the present study. Therefore such doses may be safe for daily administration without causing any serious side effects.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 321-327

 

 

Characterisation of fowl adenoviruses from chickens affected with infectious hydropericardium during 1994-1998 in India

Samadhan J Jadhao, J N Deepak , J M Kataria, R S Kataria, A K Tiwari, R Somvanshi,

P Sangamithra & K C Verma

 

Received 20 June 2002; revised 19 February 2003

In the present study characterisation has been done for six group I fowl adenoviruses (FAV) isolated from outbreaks of infectious hydropericardium (IHP) of chickens that occurred in different states/regions of India during the years 1994-98. These six viruses were identified as FAV serotype 4 by virus neutralisation and restriction endonuclease analyses. Antigenic analyses of the viruses revealed close relationship (R-values 0.93-0.96). Under the experimental conditions, we have been able to induce IHP using FAV serotype 4 isolate AD: 411 and were also able detect FAV antigens in myocardial tissues by immunofluorescence assay (a new observation), an indication that IHP causing FAV serotype 4 strain replicate in myocardial tissue. Restriction endonuclease analysis of the viral genomes (approximately 46 Kb), using Hind III, Sma I, Xba I, Bam HI, Pst I and Dra I produced identical genetic profiles. Pst I and Bam HI profiles for these six vitus isolates were identical to those published earlier for an IHP causing Pakistani FAV serotype 4 isolate KR31. The identical genetic profiles of viruses, chronology of the outbreaks of IHP in Pakistan during 1989 onward and later in Jammu and Kashmir, India (1994), suggest that FAV serotype 4 isolates involved in outbreaks of IHP in India had probably spread from Pakistan. In order to prevent further spread and economic losses due to IHP in India, based on the antigenic relatedness data in this paper, any one of the six studied FAV serotype 4 isolates can be used as a candidate for mass production of CEH culture based killed vaccine.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 328-335

 

 

Heterochrony in cotton bollworm, Earias vitella (Fb) following ethoxyprecocene administration

Irfan Ahmad Khan & Krishna Kumar

 

Received 11 March 2002; revised 5 February 2003

Administration of a synthetic precocene analogue, 7-ethoxy-6-methoxy-2,2-dimethyl chromene to fourth and last instar larvae of E. vitella results into heterochrony, viz. prothetely and metathetely. These disturbances are due to interference with the endocrine system and application of juvenile hormone to treated larvae abolishes the effect of ethoxyprecocene.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 336-340

 

 

Toxicity of PCB 1232 on mitochondria of fish Arius caelatus (Valenciennes)

Dravyam Selvarani  & C Rajamanickam

 

Received 19 February 2002, revised 24 January 2003

Mitochondrial proteins and phospholipids were estimated and SDH, Na+ - K+ - ATPase and Mg2+ - ATPase activities were analysed in the gill, liver and heart tissues of PCB 1232 (sublethal doses) treated fish A. caelatus. Protein and phospholipids were found to be decreased significantly and SDH, Na+ - K+ - ATPase, Mg2+ - ATPase and other enzyme systems displayed an inverse relationship with PCB dosage. Statistical analysis was carried out to indicate the relationship between sublethal doses of varying concentration and the activities of the enzyme systems involved in energy metabolism. The studies indicated impairment in mitochondrial functions.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 341-345

 

 

Associative diazotrophs of pearl millet (Pennisetum glaucum) from
semi arid region  Isolation and characterization

Mala Tiwari, Shashi Paroda & K R Dadarwal

 

Received 21 October 2002; revised 9 January 2003

Diversity of the native diazotrophs associated with the rhizosphere of pearl millet (P. glaucum), grown in nutritionally poor soils of semi-arid regions was studied with a view to isolate effective nitrogen fixing and plant growth stimulating bacteria with root associative characteristics. The native population varied from 103-104 g-1 of rhizosphere soil after 40 d growth and belonged to genera Azospirillum, Azotobacter and Klebsiella. Another non-diazotrophic root associative group was Pseudomonas sp., which also produced IAA and enhanced plant growth. Some of these rhizobacteria showed high in vitro acetylene reduction activity along with production of indole acetic acid. Out of 11 selected diazotrophs used as seed inoculants, M10B (Azospirillum sp.), M11E (Azotobacter sp.) and M12D4 (Klebsiella sp.) resulted in significant increase in total root and shoot nitrogen at 45 and 60 days of plant growth under pot culture conditions.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 346-351

 

 

Generation and screening of recombinant inbred lines of rice for
yellow stemborer resistance

S Mohankumar, V Thiruvengadam, K Samiayyan & P Shanmugasundaram

 

Received 8 August 2002; revised 18 February 2003

Based on the results of studies on varietal screening, antixenosis (egg laying preference) and antibiosis (larval survival and adult emergence), rice varieties W1263 and CO43 were selected as resistant and susceptible parents, respectively, for yellow stemborer (YSB) infestation. A mapping population was developed using above parents following single seed descent method. Screening for YSB reaction in F1 and F2, generations under field and glasshouse conditions for both dead hearts and white ears, established the polygenic nature of inheritance for YSB resistance. Field screening for YSB resistance at F9 generation revealed the difference in the reactions among recombinant inbred lines (RILs) between vegetative and reproductive stages. The experiments under field and glasshouse screening of RILs for dead hearts showed significant positive association. However, the reaction was more towards susceptibility in glasshouse screening due to no choice test. Scoring of 250 RILs (F8) for various morphological traits showed wide range of variation indicating the suitability for QTL mapping.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 352-356

 

 

Modulation by phenolic compounds of ABA-induced inhibition of mustard(Brassica juncea L. cv. RLM 198) seed germination

Shashi Sharma, Shanti S Sharma & Vinay K Rai

 

Received 5 June 2002; revised 21 January 2003

Exogenous abscisic acid (ABA) inhibited the germination of B. juncea  seeds in a concentration dependent manner. As revealed in a time-course study, the ABA-induced inhibition got progressively alleviated with the lapse of time following ABA treatment possibly due to metabolic conversion of applied ABA in the seed tissue. A simultaneous application of certain phenolic compounds namely, p-coumaric -, vanillic-, gallic-, and chlorogenic acid (but not caffeic acid) also caused an alleviation of ABA effect. Of the above two patterns of recovery, the phenolic-dependent alleviation of ABA effect was apparent much earlier (24-48 hr treatment) than the time-dependent one (72 hr). It is likely that phenolics could accelerate ABA metabolism in the seed tissue leading to an early recovery from ABA- induced inhibition.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 357-359

 

Notes

 

Possible role of granulocyte macrophage colony stimulating factor receptor(GM-CSF R) in malaria

Ananth N, Beena V Shetty  &  D M Vasudevan

 

Received 28 October 2002; revised 29 January 2003

Malaria has been reportedly increasing in incidence on the globe. Evidence from clinical studies supports a role for cytokines in the pathogenesis of cerebral malaria. Given the stimulatory effect of the ligand GM-CSF on the synthesis and release of the pyrogenic cytokine TNF alpha, the present study has been undertaken to investigate a possible role of GM-CSF receptor in the pathogenesis of both Plasmodium vivax and Plasmodium falciparum malaria. An enzyme immunoassay developed by us at our laboratory for the quantitation of GM-CSF receptor has been used. No changes in the concentration of the receptor have been indicated either at the time of diagnosis or after treatment. In addition, an intercomparison of the receptor concentration between the P. vivax and P. falciparum groups does not show any significant difference. The results suggest that GM-CSF receptor has no significant role in the pathogenesis of either type of malaria.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 360-362

 

 

In vitro efficacy of ciprofloxacin alone and in combination with amoxycillin against Salmonella typhi isolates

S Mandal, Manisha (Deb) Mandal & N K Pal

 

Received 18 November 2002; revised 16 January 2003

Combined effect of ciprofloxacin (Ci) and amoxycillin (Ax) has been studied in vitro against 12 clinical isolates of
S. typhi that showed Ci minimum inhibitory concentration (MIC) of ≥1 μg/ml. By agar dilution method, MIC values of Ax were 10-16 μg/ml for 11 isolates and 0.5 μg/ml for the remaining one isolate. The isolates, when treated with Ci and Ax in combination, showed fractional inhibitory concentration (FIC) of 0.004-0.256 μg/ml for Ci. FIC of Ax ranged from
6-10 μg/ml, except for a single isolate that showed Ax FIC of 0.25 μg/ml. Thus Ci was more efficacious in combination with Ax against S. typhi than Ci alone. The antibiotic combination exhibited an additive effect for all the isolates showing FIC
index 0.504-0.832.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 363-366

 

 

Antidiarrhoeal effects of methanolic root extract of Hemidesmus indicus (Indian sarsaparilla)  An in vitro and in vivo study

Sarita Das, R Prakash & S Niranjali Devaraj

 

Received 7 October 2002; revised 5 February 2003

Methanolic extract of H. indicus root (MHI) was screened for its antimicrobial activity against S. typhimurium, E. coli and S. flexneri, in vitro and in experimentally induced diarrhoea in albino rats, in vivo. MHI had an anti enterobacteriae effect as evident from agar well diffusion method and decrease in CFU/ml in MHI treated LB broth culture. MHI inhibited the castor oil induced diarrhoea in rats as judged by a decrease in the amount of wet faeces in MHI-pretreated rats at a dose of 500-1500 mg/kg. The results indicated that MHI was more active than standard antidiarrhoeal drug, lomotil. Phytochemical tests revealed the main constituents as tannins, steroids, triterpenoids and carbohydrates. Present findings suggested that MHI might elicit an antidiarrhoeal effect by inhibition of intestinal motility and by its bacteriocidal activity.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 367-369

 

 

Protection of adrenocortical activity by dietary casein in ether anaesthetized rats

N M Biswas, A Chattopadhyay & M Sarkar

 

Received 31 July 2002; revised 29 January 2003

Adrenal D5-3b-hydroxysteroid dehydrogenase (D5-3b-HSD) activity and serum corticosterone level were significantly higher in rats fed with 5% casein or 4% albumin diets after 1 hr of ether anaesthetic stress as compared to the controls, 5% casein and 20% casein (equivalent to 4% albumin) respectively. Ether anaesthesia to 20% casein fed rats caused no change in adrenal D5-3b-HSD activity and serum corticosterone level when compared with controls fed 20% casein diet. The results suggest that high milk protein diet may prevent acute stress effects by protecting adrenocortical activity. The present investigation opens up a new area of management of stress.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 370-372

 

 

Effect of sodium glycocholate on development of tolerance to
Parthenium hysterophorus extracts

Arshia Shariff,Sarasija Suresh & K L K Paranjothy

and

K V Nagendra Prasad

 

Received 1 April 2002; revised 19 February 2003

 

Sodium glycocholate, a penetration enhancer showed significant enhancement of IgG levels in Balb/c mice when administered intranasally with P. hysterophorus pollen extract.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 373-375

 

 

Amylase induction during seed germination in isoproturon susceptible andresistant biotypes of Phalaris minor Retz.

R S Dhawan, A K Dhawan, S Kajla & R Moudgil

 

Received 16 May 2002; revised 11 February 2003

Isoproturon resistant biotype of P.minor germinates early, shows higher germination percentage and faster rate of growth as compared to the susceptible biotype. Higher amylase activity is observed in the initial hours of imbibition in the resistant biotype. In the susceptible biotype it is activated at a much later stage.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 376-378

 

 

Evaluation of entomopathogenic nematode, Steinernema feltiae against fieldpopulation of mustard sawfly,
Athalia lugens proxima (Klug) on radish

K Narayanan

 and

C Gopalakrishnan

 

Received 19 July 2002; revised 4 February 2003

Mustard sawfly, A. lugens proxima, was found to be highly susceptible to entomopathogenic nematode, S. feltiae under laboratory condition. Application of three different doses of S. feltiae, viz. 1.1  103, 1.1  104 and 1.1  105 infective juveniles/ml, at weekly intervals, significantly reduced the field population of mustard sawfly on radish. The mean larval population of A. lugens proxima in all doses of nematode treated plots ranged from 0.42 to 0.48 larvae per plant as against 2.95 larvae / plant in untreated control plots. Similarly, the yield of radish in all the nematode treated plots was significantly higher by way of recording 2.80 to 2.87 tons/ ha as compared to 1.63 tons/ha in the case of control.

 

Indian Journal of Experimental Biology

Vol. 41, April 2003, pp. 379-382

 

 

Modified behaviour in nucleopolyhedro virus infected field bean pod borer, Adisura atkinsoni and its impact on assessing the field efficacy of NPV

K Narayanan

 

Received 31 October 2002; revised 4 February 2003

When nucleopolyhedro virus of A.atkinsoni was applied at 250 LE/ha, there was no significant difference between the viruses treated and control plots with regard to the total number of live larvae feeding outside the pod. However, due to changes in behaviour in NPV infected A. atkinsoni by way of coming out of the pod, there was a significant difference when counts were taken with regard to total number of larvae found feeding inside the pod. Both endosulfan at (0.07%) and virus (125 LE/ha) in combination with endosulfan (0.035%) significantly reduced the larval population of A. atkinsoni and Sphaenarches anisodactylus. There was no significant difference between the virus and control plots with regard to percentage of pod damage. However, when the yield was assessed based on the seed weight, there was significant difference.

 

AUTHOR INDEX

Agarwal Rachna

290

Nair Sudhakaran C R

296

Agrawal V K

304

Narayanan K

376, 379

Ananth N

357

Augusti K T

296

Paranjothy K L K

370

 

 

Pal N K

360

Bandyopadhyay Sandip K

311

Paroda Shashi

341

Biswas N M

367

Poddar Mrinal K

283

 

 

Prabha T

304

Chakrabarty Asit K

290

Prakash R

363

Chattopadhyay A

367

Prasad Nagendra K V

370

Chetana M

316

Priyambada S

304

 

 

 

 

Dadarwal K R

341

Daniel Regi Susan

296

 Rai Vinay K

352

Das Sarita

363

Rajamanickam C

336

Deepak J N

321

Ray Arun

311

Devaraj Niranjali S

363

Ray Chaudhuri Susri Guha

311

Devi K s

296

Dhawan A K

373

Samiayyan K

346

Dhawan R S

373

Sangamithra P

321

Dora Babu M

304

Sarkar M

367

 

 

Selvarani Dravyam

336

Goel R K

304

Shanmugasundaram P

346

Gopalakrishnan C

376

Shariff Arshia

370

 

 

Sharma Shanti S

352

Jadhao Samadhan J

321

Sharma Shashi

352

 

 

Shetty Beena V

357

Kajla S

373

Somvanshi R

321

Kataria J M

321

Sulochana B

316

Kataria R S

321

Suresh Sarasija

370

Khan Irfan Ahmad

328

Kumar Krishna

328

Thiruvengadam V

346

 

 

Tiwari A K

321

Majumdar Biswajit

311

Tiwari Mala

341

Mandal Manisha (Deb)

360

Tripathi A K

290

Mandal S

360

 

 

Mohankumar S

346

Udupa N

316

Mondal Anup

283

Uma Devi P

316

Moudgil R

373

 

 

Mukhopadhyay Sutapa (Kali)

283

Vasudevan D M

357

Mutalik S

316

Verma K C

321