Indian Journal of Experimental Biology

 

Total visitors: 1,419  since 17-10-06

VOLUME 44

NUMBER 10

OCTOBER 2006

CODEN : IJEB (A6) 44(10) 773-856 (2006)

ISSN : 0019-5189

 

 

CONTENTS

Minireview

 

Is the anti-sarcoma and anti-viral cytokine “plasma factor” a novel chicken
Y-box protein?

777

      Manohar V N Shirodkar & Pravin B Sehgal

 

 

 

Papers

 

Binding characteristics and distribution of lactoferrin receptors in the gut and choroid plexus in newborn calves

783

      M J R Talukder & E Harada

 

 

 

Modulation of lecithin activity by vitamin-B complex to treat long term consumption of ethanol induced oxidative stress in liver

791

      Subir Kumar Das & D M Vasudevan

 

 

 

Heat stress induced apoptosis in BC-8 cells derived from AK-5 tumor involves downregulation of Bcl-2 and generation of reactive oxygen species

802

      Ashok Khar, A Leela Kumari, B V V Pardhasaradhi, Ch Varalakshmi &
Nandini Rangaraj

 

 

 

Involvement of nitric oxide (NO) in the regulation of stress susceptibility and
adaptation in rats

809

      Kavita Gulati, Arunabha Ray, Anbrin Masood & V K Vijayan

 

 

 

Role of free radicals in stress-induced neurobehavioural changes in rats

816

      Rishi Pal, Kavita Gulati, Ayanabha Chakraborti, Basudeb Banerjee & Arunabha Ray

 

 

 

Protective effect of ethanolic and water extracts of sea buckthorn (Hippophae rhamnoides L.) against the toxic effects of mustard gas

821

      R Vijayaraghavan, Anshoo Gautam, Om Kumar, S C Pant, Manoj Sharma, Seema Singh, H T Satish Kumar, Anand Kumar Singh, Manisha Nivsarkar, M P Kaushik,
R C Sawhney, O P Chaurasia & G B K S Prasad

 

 

 

Antioxidant property of Decalepis hamiltonii Wight & Arn

832

      K N Chidambara Murthy, T Rajasekaran, P Giridhar & G A Ravishankar

 

 

 

Ginseng extract exhibits antimutagenic activity against induced mutagenesis in various strains of Salmonella typhimurium

838

      Thiraviam Geetha, Amarpreet Saini & Indu Pal Kaur

 

 

Optimization of nutritional requirements for gentamicin production by
Micromonospora echinospora

842

      M Himabindu & Annapurna Jetty

 

 

 

Iron induced metabolic changes in the diazotrophic cyanobacterium Anabaena PCC 7120

849

      Rishi Kumar Saxena, Ruchika Raghuvanshi, Surendra Singh & Prakash Singh Bisen

 

 

 

Short Communication

 

DNA isolation from goat blood using different brands of household detergents and its downstream application

852

      O Suneel Kumar, M K Sharma & Dheer Singh

 

 

 

Book Review

 

Recent progress in medicinal plants—Plant bioactives in traditional medicine

855

      G D Bagchi, J Singh, Suchitra Banerjee, M M Gupta & S P S Khanuja

 

 

 

Announcements

776

National Seminar on Molecules from Ayurvedic Metal and Mineral Preparations, and Workshop on Standard Operational Procedure (SOP) on Ayurvedic Rasausadhis

 

Drs Anant and Lata Labhsetwar Charitable Trust, and Labhsetwar Foundation, USA-Award announcement

 

——————————

Announcements

 

National Seminar on Molecules from Ayurvedic Metal and Mineral Preparations

and

Workshop on Standard Operational Procedure (SOP) on Ayurvedic Rasausadhis

2-4 December 2006, Science City, Kolkata

 

        Organised by the Paschim Banga Ayurved Chikitsak Samity, State Committee, West Bengal, Kolkata, in collaboration with J B Roy State Ayurvedic Medical College & Hospital, Kolkata, Central Research Institute (Ayurveda), Kolkata, Institute of Postgraduate Ayurvedic Education & Research, Kolkata, Viswanath Ayurved Mahavidyalaya & Hospital, Kolkata and Government Ayurvedic Doctors Association, Kolkata, the seminar will disseminate the age-old traditional concept of Ayurvedic metal preparation, their uses, updated scientific knowledge, standardization, safety study etc. and the workshop will cover the topics like practical demonstration on bhavna of dhatus, baddha musa, putapka, gajaputa, muffle furnace, examination of bhasma etc. For details kindly contact Dr Tuhin Kanti Biswas, Secretarial-in-Charge, J B Roy State Ayurvedic Medical College & Hospital, 170-172, Raja Dinendra Street, Kolkata 700 004. Telephone: 033-25335019, Mobile: 9830232395/ 9433173272; e-mail: ayurvedchikitsaksamity@rediffmail.com

 

——————————

 

Drs Anant and Lata Labhsetwar Charitable Trust, and Labhsetwar Foundation, USA

Award announcement

 

With a view to encourage and recognize the work of those who have made significant contributions towards India’s population stabilization issues, Drs. Anant and Lata Charitable Trust, Nagpur and Labhsetwar Foundation USA (e-mail: latalabh@aol.com) have established a Population Control and Family Welfare Award. The award consisting of Rs. 1,00,000/= in cash, a memento and a certificate is given annually to an individual of Indian nationality who has made outstanding research contributions in this fields of physiology, immunology, pharmacology, clinical, social, operational and behavioural work and demography. The Committee has the right to split the award in the event found necessary. Nominee should be under 55 years of age. Nominations giving complete biodata of the candidate along with list of publications and reprints of the five best publications in the related field should be sent to: Dr. C.P. Puri, Director, National Institute for Research in Reproductive Health, Jehangir Merwanji Street, Parel, Mumbai 400 012, latest by 1 November 2006. The award function will be held in February 2007.

 

 

 

 

AUTHOR INDEX

Bagchi G D

856

Banerjee Basudeb

816

Banerjee Sucitra

856

Bisen Singh Surendra

849

 

 

Ch Varalakshmi

802

Chakraborti Ayanabha

816

Chaurasia O P

821

 

 

Das Subir Kumar

791

 

 

Gautam Anshoo

821

Geetha Thiraviam

838

Giridhar P

832

Gulati Kavita              09, 816

809, 816

Gupta M M

856

 

 

Harada E

783

Himabindu M

842

 

 

Jetty Annapurna

842

 

 

Kaur Indu Pal

838

 

 

Kaushik M P

821

Khanuja S P S

856

Khar Ashok

802

Kumar H T Satish

821

Kumar O Suneel

852

Kumar Om

821

Kumari A Leela

802

 

 

Masood Anbrin

809

Murthy K N Chidambara

832

 

 

Nivsarkar Manisha

821

 

 

Pal Rishi

816

Pant S C

821

Pardhasaradhi B V V

802

Prakash Singh

849

Prasad G B K S

821

 

 

Raghuvanshi Ruchika

849

Rajasekaran T

832

 

 

Rangaraj Nandini

802

Ravishankar G A

832

Ray Arunabha

809, 816

 

 

Saini Amarpreet

838

Sawhney R C

821

Saxena Rishi Kumar

849

Sehgal Pravin B

777

Sharma M K

852

Sharma Manoj

821

Shirodkar Manohar V N

777

Singh Anand Kumar

821

Singh Dheer

852

Singh J

856

Singh Seema

821

 

 

Talukder M J R

783

 

 

Vasudevan D M

791

Vijayan V K

809

Vijayaraghavan R

821

 

 

KEYWORD INDEX

Adaptation

809

AK-5 tumor

802

Ames test

838

Anabaena

849

Antimutagenicity

838

Antioxidant

816, 832

Anti-sarcoma

777

Apoptosis

802

Aromatase

852

 

 

Bcl-2

802

Binding characteristics

783

 

 

Calves

783

Choroid plexus

783

Cynobacterium

849

Cytokine

777

 

 

Decalepis hamiltonii

832

Detergent

852

DNA isolation

852

DPPH assay

838

 

 

 

 

 

 

Elevated plus maze

816

Emotionality

809

Ethanol

791

 

 

Free radicals

816

 

 

Gentamicin production

842

Ginseng extract

838

Glutathione

821

Gut

783

 

 

Heat shock

802

Hippophae rhamnoides

821

2-Hydroxy-4-methoxy

 

 benzaldehyde

832

 

 

In vitro antioxidant action

838

Iron

849

 

 

Lactoferrin receptors

783

Lecithin

791

Liver

791

 

 

Malondialdehyde

821

Metabolic change

849

Micromonospora echinospora

 

 

 

Nitric oxide

809

Nutritional requirement

842

 

 

Open field

816

Oxidative stress

791, 821

 

 

PCR

852

Plasma factor

777

 

 

Restraint stress

816

ROS

802

 

 

Stress

809

Sulphur mustard

821

 

 

Tocopherol

791

 

 

Vitamin-B Complex

791

 

 

Y-box protein

777

 

 

Indian Journal of Experimental Biology

Vol. 44, October 2006, pp. 777-782

 

 

 

Minireview

 

Is the anti-sarcoma and anti-viral cytokine “plasma factor” a novel chicken
Y-box protein?ψ

Manohar V N Shirodkar & Pravin B Sehgal

 

A line of research beginning in the early 1960s with the observation that West Nile virus and, later, several strains of rabies virus could inhibit the development of the Rous sarcoma virus-induced tumor in the wing-web of chicken (a “sarcoma-blockade”) eventually culminated in the characterization of a 14-kDa circulating anti-sarcoma and anti-viral activity christened “plasma factor” (PF) which, unlike the interferons, inhibited the replication of diverse RNA-containing viruses, but not of any DNA-containing viruses. The possibility that this 14 kDa protein represented a novel antiviral cytokine has been strengthened by analysis of partial amino acid sequencing data which suggest that this 14-kDa cytokine may correspond to the 127-amino acid-long chicken YB2-like protein (Locus: XP_423576) deduced very recently from the genomic sequencing of chicken. Biologically, proteins of the Y-box family (such as chicken YB1 and YB2) not only bind DNA and thus regulate transcription but also bind single-stranded RNA in a sequence-specific and reversible manner, repress viral RNA translation, inhibit retroviral transformation of chicken fibroblasts, and are known to regulate transcription of human immunodeficiency virus and hepatitis B virus. Taken together, the available data point to a novel anti-viral cytokine with a novel mechanism of action.

 

 

Indian Journal of Experimental Biology

Vol. 44, October 2006, pp. 783-790

 

Papers

Binding characteristics and distribution of lactoferrin receptors
in the gut and choroid plexus in newborn calves

M J R Talukder & E Harada

Received 13 May 2005; revised 7 August 2006

Lactoferrin (Lf), an iron-binding multifunctional glycoprotein, abundantly present in colostrum and milk of different species such as humans, bovines, and mice has been shown that bovine colostral Lf is transported into the CSF via plasma in newborn calves. Specific Lf-receptors (Lf-R) are present in different cells of different species. In the present study, we report for the first time, the presence and distribution of Lf-R in the intestine and choroid plexus in newborn calves. Brush-border membrane vesicles (BBMV) were prepared from the mucosa of duodenum, jejunum, ileum, colon, epithelium overlying Peyer's patches (EOPP) in jejunum (EOPPJ) and ileum (EOPPI), and choroid plexus membranes. Receptor binding assays were carried out using 125I labeled bovine Lf. Specific and saturable Lf-R were found in BBMV of
all the
intestinal segments and choroid plexus examined. Nonlinear regression and Scatchard plot analyses clearly revealed that EOPP had the highest binding maximal (Bmax), and lowest in colon. The maximum dissociation constant (Kd) 0.7 µM was in colon while, Bmax and Kd in choroid plexus membrane were 16.87 nmol/mg protein and 0.34 µM, respectively. All these findings together strongly suggested that Lf was transported into CSF via plasma through receptor mediated transcytosis.

 

 

Indian Journal of Experimental Biology

Vol. 44, October 2006, pp. 791-801

 

 

Modulation of lecithin activity by vitamin-B complex to treat long term consumption of ethanol induced oxidative stress in liver

Subir Kumar Das & D M Vasudevan

 

Received 31 October 2005; revised 20 June 2006

Alcoholic liver disease (ALD) develops as a consequence of priming and sensitizing mechanisms rendered by cross-interactions of primary mechanistic factors and secondary risk factors. Chronic alcohol abuse and its progression to ALD are associated with abnormal metabolism and low tissue or plasma levels, or both, of many micronutrients. Glutathione depletion is considered the most important sensitizing mechanism. In the present study efficacy of lecithin with vitamin-B complex to treat ethanol induced oxidative stress was compared with the effect of lecithin alone, tocopheryl acetate (vitamin E), as well as capacity of hepatic regeneration during abstention. Ethanol (1.6g / kg body weight/ day for 4 weeks) affects body weight in 16-18 week old male albino rats of Wistar strain weighing 200-220 g. Thiobarbituric acid reactive substance level, nitrite content, protein carbonyl group level, redox ratio (oxidized to reduced glutathione ratio), superoxide dismutase activity, and glutathione s-transferase activity significantly increased on ethanol exposure. Whereas reduced glutathione content, and activities of catalase, glutathione reductase and glutathione peroxidase significantly reduced due to ethanol exposure. These changes were reversed by different treatment. The results suggest that tocopheryl acetate (vitamin E) could partially reverse these changes and act as a potential therapeutic agent. However, lecithin with vitamin-B complex treatment is a promising therapeutic approach. Furthermore, preventive measures were more effective than curative treatment. Prevention of oxidative and nitrosative stress along with correction of nutritional deficiency is one of the proposed mechanisms for the therapeutic approach.

 

 

Indian Journal of Experimental Biology

Vol. 44, October 2006, pp. 802-808

 

 

Heat stress induced apoptosis in BC-8 cells derived from AK-5 tumor involves downregulation of Bcl-2 and generation of reactive oxygen species

Ashok Khar, A Leela Kumari, B V V Pardhasaradhi, Ch Varalakshmi & Nandini Rangaraj

Received 20 February 2006; revised 23 June 2006

BC-8, a rat histiocytoma undergoes apoptosis after heat shock, which is due to lack of an effective heat shock response. Heat shock induced generation of free radicals, which in turn are involved in the induction of apoptotic death in BC-8 cells. Treatment of BC-8 cells with N-acetylcysteine partially inhibited the heat induced apoptosis. Introduction of Bcl-2 gene in these cells did not protect them from apoptotic death, whereas transfection with hsp-70 gene did render these cells resistant to heat induced apoptosis transiently. Heat shock also downregulated the expression of Bcl-2 and p53 in these cells. These observations suggested that the heat shock induced apoptosis was mediated through reactive oxygen species and controlled upstream of Bcl-2 check point.

 

 

Indian Journal of Experimental Biology

Vol. 44, October 2006, pp. 809-815

 

 

Involvement of nitric oxide (NO) in the regulation of stress susceptibility and adaptation in rats

Kavita Gulati, Arunabha Ray, Anbrin Masood & V K Vijayan

 

Received 5 June 2006; revised 21 July 2006

The present study evaluated the regulatory role of nitric oxide (NO) in stress susceptibility and adaptation in rats. Acute restraint stress (RS ×1) reduced the number of entries and time spent in the open arms in the elevated plus maze (EPM) test and raised plasma corticosterone levels. RS (×1)-induced neurobehavioral suppression and raised corticosterone levels were attenuated by pretreatment with the NO precursor, L-arginine (500 and 1000 mg/kg)and unaffected or further aggravated by NO synthase inhibitor, L-NAME or 7-nitroindazole (10 and 50 mg/kg). Biochemical assay of plasma and brain homogenates showed that these RS – induced behavioral and neuroendocrinal changes were associated with lowered levels of plasma and brain total nitrates/nitrites (NOx). L-Arginine attenuated the RS-induced suppression of NOx levels in plasma and brain, whereas, the NO synthase inhibitors tended to produce reverse effects. In the experiments involving repeated stress i.e. RS (×5), exposure resulted in attenuation/reversal of (a) neurobehavioral suppression in the EPM test and (b) lowered brain NOx, that was seen after RS (×1). The RS (×5)-induced changes in EPM parameters and brain Nox were further potentiated after L-arginine pretreatment, whereas, the NO synthase inhibitors were less effective. Rats were screened as high and low emotional in the open-field test, and high emotional rats showed greater(a) behavioral suppression in the EPM, (b) corticosterone responses (c) brain NOx suppression, and (d) cold-restraint stress (CRS) induced gastric mucosal lesions as compared to their low emotional counterparts. L-Arginine pretreatment was more effective in modulating the above RS induced stress responses/markers in the high emotional group of rats. Our data suggest that NO plays a differential role during exposure to acute and repeated stress situations, and that the relationship between stress and emotionality status may be under the regulatory influence of NO.

 

Indian Journal of Experimental Biology

Vol. 44, October 2006, pp. 816-820

 

 

Role of free radicals in stress-induced neurobehavioural changes in rats

Rishi Pal, Kavita Gulati, Ayanabha Chakraborti, Basudeb Banerjee1 & Arunabha Ray

Received 14 February 2006; revised 5 June 2006

Effect of restraint stress (RS) and its modulation by antioxidants were evaluated on elevated plus maze (EPM) and open field (OF) tests in rats. Restraint stress (RS for 1hr) reduced the number of open arm entries, as also the time spent on open arms indicating enhanced anxiogenic response in the EPM test as compared to normal non RS group of rats. Pretreatment with ascorbic acid (100 and 200 mg/kg) and a-tocopherol (30 and 60 mg/kg) attenuated these RS-induced effects. In the OF test, RS-reduced (a) ambulations; and (b) rearings, whereas an increase was seen in (a) latency of entry and (b) number of fecal boluses. The RS-induced changes in OF parameters were reversed after pretreatment with the antioxidants, (ascorbic acid and alpha tocopherol). Biochemical data showed that RS enhanced MDA levels in both serum and brain, and these were attenuated after pretreatment with the antioxidants. The pharmacological and biochemical results indicate that free radicals might be involved in such stress-induced neurobehavioural effects.

 

 

Indian Journal of Experimental Biology

Vol. 44, October 2006, pp. 821-831

 

 

Protective effect of ethanolic and water extracts of sea buckthorn (Hippophae rhamnoides L.) against the toxic effects of mustard gas

R Vijayaraghavan, Anshoo Gautam, Om Kumar, S C Pant, Manoj Sharma, Seema Singh,
H T Satish Kumar, Anand Kumar Singh, Manisha Nivsarkar, M P Kaushik, R C Sawhney,
O P Chaurasia & G B K S Prasad

 

Received 23 December 2005; revised 7 June 2006

Ethanolic extract of H. rhamnoides L. leaf (HL-EOH), water and ethanolic extract of H. rhamnoides fruit (HF-W and HF-EOH), and H. rhamnoides flavone from fruit (HR-flavone) were evaluated against percutaneously administered sulphur mustard (SM), a chemical warfare agent. The animals administered with SM (9.7, 19.3 and 38.7 mg/kg) died at various days depending upon the dose and there was a significant reduction in the body weight. The H. rhamnoides extracts (1g/kg; 3 doses; po) significantly protected the lethality, with a protective index of 2.4, 1.7, 1.7 and 2.2 for HL-EOH, HF-W, HF-EOH and HR-flavone respectively. Reduced glutathione (GSH) and oxidized glutalthione (GSSG) levels were reduced, and malondialdehyde (MDA) was elevated after percutaneous administration of SM. Oral administration of HL-EOH and HR-flavone significantly protected the body weight loss. Recovery in the levels of GSH, GSSG and MDA were also observed following oral administration of HL-EOH and HR-flavone. All the extracts were non-toxic and the LD50 was more than 5 g/kg. The present study shows that percutaneous administration of SM induces oxidative stress and ethanolic extract of leaf of H. rhamnoides and H. rhamnoides flavone from fruit can significantly protect it.

 

 

Indian Journal of Experimental Biology

Vol. 44, October 2006, pp. 832-837

 

 

Antioxidant property of Decalepis hamiltonii Wight & Arn

K N Chidambara Murthy, T Rajasekaran, P Giridhar & G A Ravishankar

 

Received 2 January 2006; revised 19 June 2006

Aromatic edible root of D. hamiltonii was subjected to the extraction of the antioxidant rich fraction. Different parts of root namely whole tuber, peel, tuber without peel and medullary portion were extracted with dichloromethane (European Patent No. W02005063272). The extract was found to contain flavor compound 2-hydroxy-4-methoxybenzaldehyde (2H4MB), which was identified by TLC and GC. Medullary portion was found to be rich in 2H4MB, (73.73 mg g-1dry tissue) followed by peel, containing 68.34 mg g-1 2H4MB. Different concentration of dichloromethane extracts were subjected for antioxidant assay by DPPH (1,1 dihydroxy 2-picryl hydrazyl) method, this has shown 44, 46.7% radical scavenging activity in case of medullary, peel extracts and 67.3% in case of pure 2-hydroxy-4-methoxybenzaldehyde at 100 ppm concentration, whereas ascorbic acid used as standard showed 94.3% activity. In b-carotene linoleate model system (b-CLAMS) 43.46 and 45.7% antioxidant activity was observed in medullary and peel extracts at 100 ppm concentrations respectively, whereas standard 2-hydroxy-4-methoxybenzaldehyde exhibited 69.64% at 100 ppm and BHA (butylated hydroxyl anisole) 90.1% activity also at 100-ppm level. Similarly hydroxyl radical scavenging activity was found to be 48.36, 46.86, 48.26 and 73.60% in whole tuber, medullary, peel and standard 2-hydroxy-4-methoxy benzaldehyde respectively at 100 ppm levels. This is the first report on the antioxidant activity of D. hamiltonii. Results have shown that 2H4MB is one of the major constituents responsible for antioxidant activity. Hence the extract of D. hamiltonii can be utilized for the production of antioxidant rich fractions required for various health benefits.

 

 

Indian Journal of Experimental Biology

Vol. 44, October 2006, pp. 838-841

 

 

Ginseng extract exhibits antimutagenic activity against induced mutagenesis in various strains of Salmonella typhimurium

Thiraviam Geetha, Amarpreet Saini & Indu Pal Kaur

 

Received 10 February 2005; revised 26 July 2006

Ginseng has been reported to exhibit antioxidant and antimutagenic activity. The present study was undertaken with a view to confirm whether the antioxidant activity of Ginseng is responsible for its antimutagenic action. The concentrated root extract of Panax ginseng (Ginseng extract I) and its lyophilized powder (Ginseng extract II) obtained from two different manufacturing houses, were tested against mutagenesis using the well-standardized Ames microsomal test system. The extracts exhibited antimutagenic effect against hydrogen peroxide induced mutagenesis in TA100 strain, and against mutagenesis produced by 4-nitroquinoline-N-oxide in both TA98 and TA100 strains of Salmonella typhimurium. Both the extracts failed to show any antimutagenic potential against tert-butyl hydroperoxide (an oxidative mutagen) in TA102 strain, a strain highly sensitive to active oxygen species. The extracts also indicated a weak antioxidant activity in a series of in vitro test systems viz., 1,1-diphenyl picryl hydrazyl (DPPH) assay, hydrogen peroxide scavenging and superoxide anion scavenging. The results indicate that the protective effects shown by ginseng extract(s) against 4-nitroquinoline-n-oxide and hydrogen peroxide induced mutagenesis in TA98 and TA100 could mainly be due to its property to initiate and promote DNA repair rather than free radical scavenging action.

 

 

Indian Journal of Experimental Biology

Vol. 44, October 2006, pp. 842-848

 

 

Optimization of nutritional requirements for gentamicin production by Micromonospora echinospora

Himabindu M & Annapurna Jetty

 

Received 5 January 2006; revised 2 June 2006

Effect of various fermentation media, carbon sources, nitrogen sources, phosphate concentration and culture requirements includes inoculum levels and age were determined on gentamicin production and biomass dry weight production for Micromonospora echinospora, a gentamicin producing strain. Of the substrates tested, starch as a sole carbon source promoted maximal gentamicin production, while maltose promoted maximal growth. Yeast extract as a sole nitrogen source promoted maximal growth, while soyabean meal for gentamicin production. Increasing phosphate concentration enhanced gentamicin production and observed optimum production at 1.2g/l (6% v/v) of phosphate having 72 h old inoculum in the medium. Highest gentamicin production was obtained after cultivation with shaking for 120 h in a medium containing starch 0.75%(w/v), soyabean meal 0.5%, K2HPO4 0.12%, CaCO3 0.4%, FeSO4 0.003% and CoCl2 0.0001%. The gentamicin production was 1.2-fold in this medium as compared to basal medium.

 

 

 

Indian Journal of Experimental Biology

Vol. 44, October 2006, pp. 849-851

 

 

Iron induced metabolic changes in the diazotrophic cyanobacterium
Anabaena PCC 7120

Rishi Kumar Saxena

 

Ruchika Raghuvanshi & Surendra Singh

 

Prakash Singh Bisen

 

Received 20 January 2006; revised 24 April 2006

Iron induced changes in growth , N2-fixation, CO2 fixation and photosynthetic activity were studied in a diazotrophic cyanobacterium Anabaena PCC 7120. Iron at 50 µM concentration supported the maximum growth, heterocyst frequency, CO2 fixation, photosystem I (PS I), photosystem II (PS II) and nitrogenase activities in the organism. Higher concentration of iron inhibited these processes. Chl a and PS II activities were more sensitive to iron than the protein and PS I activity.

 

 

Indian Journal of Experimental Biology

Vol. 44, October 2006, pp. 852-854

 

 

Short Communication

DNA isolation from goat blood using different brands of household detergents and its downstream application

O Suneel Kumar, M K Sharma & Dheer Singh

 

Received 13 November 2005; revised 17 July 2006

Rapid isolation of DNA from goat blood using different brands of detergents available in Indian market, is reported. The integrity and efficiency of these DNA preparations were compared with genomic DNA isolated by a standard kit (Flexi gene DNA kit), using amplification of exon 2 of CYP19 (aromatase) gene. The similar and significant amplification of this gene was obtained using genomic DNA isolated by kit and various detergents. However, among the detergents used, the Rin and Ezee were found to be the best to get DNA of high purity comparable to that obtained by kit.

 

 

 

Indian Journal of Experimental Biology

Vol. 44, October 2006, pp. 855-856

 

 

Book Review

 

Recent progress in medicinal plants—Plant Bioactives in traditional medicine

By G D Bagchi, J Singh, Suchitra Banerjee,M M Gupta and S P S Khanuja