Indian Journal of Experimental Biology

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VOLUME 46

NUMBER 2

FEBRUARY 2008

CODEN: IJEB (A6) 46(2) 85-144 (2008)

ISSN: 0019-5189

 

CONTENTS

 

Mini Review

 

Newer insights into renal regulation of water homeostasis

89

Manoj G Tyagi & J Nandhakumar

 

 

 

Papers

 

Detection of anti-Haemonchus contortus antibodies in sheep by dot-ELISA with immunoaffinity purified fraction of ES antigen during prepatency

94

A Prasad, A Nasir & Nem Singh

 

 

 

Pathological studies on Bovine papilloma virus-fern interaction in hamsters

100

G D Leishangthem, R Somvanshi & D R Lauren

 

 

 

Lycopene ameliorates thermal hyperalgesia and cold allodynia in STZ-induced
diabetic rat

108

Anurag Kuhad & Kanwaljit Chopra

 

 

 

Efficiency of propolis extract against mitochondrial stress induced by antineoplastic agents (doxorubicin and vinblastin) in rats

112

Lamia Benguedouar, Hanane Nadia Boussenane, Wided Kebsa, Mohamed Alyane, Hassiba Rouibah & Mesban Lahouel

 

 

 

In vitro antiviral activity of plant extracts on goatpox virus replication

120

V Bhanuprakash, M Hosamani, V Balamurugan, P Gandhale, Ram Naresh,
D Swarup & R K Singh

 

 

 

Anti-microfilarial activity of methanolic extract of Vitex negundo and Aegle marmelos and their phytochemical analysis

128

K N Sahare, V Anandhraman, V G Meshram, S U Meshram, M V R Reddy,
P M Tumane & K Goswami

 

 

 

Ameliorative effect of PartySmart in rat model of alcoholic liver disease

132

S Gopumadhavan, Mohammed Rafiq, M Azeemuddin & S K Mitra

 

 

 

Nutritional regulation of actinomycin-D production by a new isolate of Streptomyces sindenensis using statistical methods

138

Vandana Praveen, Divya Tripathi, C K M Tripathi & Vinod Bihari

 

 

 

Announcement

 

National Seminar on Application of Bioinformatics in Genome Research

88

 

 

 

Announcement

 

National Seminar on Applications of Bioinformatics in Genome Research

13-15 February 2008, Pondicherry

 

Sponsored by the Department of Biotechnology, Govt. of India, the Seminar is being organized by the Centre for Bioinformatics, School of Life Sciences, Pondicherry University.
The focal themes of the seminar include, but are not limited to: Molecular sequence analysis, Recognition of genes and regulatory elements, Computing molecular evolution, Gene expression & molecular networks, Functional linkage analysis, Computational proteomics, Structure based functional analysis, Micro array, Molecular dynamics simulation, and Combinatorial libraries and drug design. For details please contact: Prof. P. P. Mathur, Coordinator, Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Pondicherry 605 014.
Phone : (0413) 2655212; Fax: (0413) 2655211; E-mail: bicpu2001@yahoo.co.in

 

覧覧覧覧覧覧

 

NISCAIR Policy on Plagiarism

 

The system of formal communication in science through publication in primary journals is based on originality and quality of information being the only criteria for publication. However, there have been tendencies to misuse the system and vitiate the process of science communication for personal benefits. One of the ills afflicting science communication is plagiarism. Attempts at plagiarism may range from verbatim copying of extensive material of other authors, misappropriating results/data of others with minor changes in language/presentation without giving credit to original source, to publish essentially the same information more than once.

As the premier publisher in India of primary scientific journals in various disciplines of science and technology, NISCAIR strongly reiterates its policy of discouraging plagiarism of all kinds. All efforts are made detect and frustrate attempts at plagiarism through editorial screening and rigorous peer review in respect of communications received for publication in NISCAIR publications. Cooperation of the scientific community is sought in our efforts to frustrate all attempts at plagiarism.

In case any attempt to plagiarize is brought to our attention accompanied with convincing evidence, following steps would be taken:

(a)          After consulting the respective Editorial Board Members, authors guilty of plagiarism will be debarred from publishing their papers in NISCAIR journals.

(b)          Heads of the departments/institutes of the offending authors will be intimated of such incidences of plagiarism.

(c)          Such incidents of plagiarism will be publicized through the concerned NISCAIR journals in consultation with the respective Editorial Board Members.

 

 

 

Author Index

Alyane Mohamed

112

Anandhraman V

128

Azeemuddin M

132

Balamurugan V

120

Benguedouar Lamia

112

Bhanuprakash V

120

Boussenane Hanane Nadia

112

Chopra Kanwaljit

108

Gandhale P

120

Gopumadhavan S

132

Goswami K

128

Hosamani M

120

Kebsa Wided

112

Kuhad Anurag

108

Lahouel Mesban

112

Lauren D R

100

Leishangthem G D

100

Meshram S U

128

Meshram V G

128

Mitra S K

132

Nandhakumar J

89

Nasir A

94

Prasad A

94

Praveen Vandana

138

Rafiq Mohammed

132

Ram Naresh

120

Reddy M V R

128

Rouibah Hassiba

112

Sahare K N

128

Singh Nem

94

Singh R K

120

Somvanshi R

100

Swarup D

120

Tripathi C K M

138

Tripathi Divya

138

Tumane P M

128

Tyagi Manoj G

89

Vinod Bihari

138

 

 

 

Keyword Index

Aquaporin 2

89

Acacia arabica

120

Actinomycin-D

138

Aegle marmelos

128

Alcohol

132

Antifilarial activity

128

Antiviral activity

120

BPV-2

100

Calcineurin

89

Diabetic neuropathy

108

Dot ELISA

94

Doxorubicin

112

Eugenia jambolana

120

Excretory-secretory antigen

94

Fermentationg

138

Fern

100

Goatpox virus

120

Haemonchus contortus

94

Hamster

100

Heart

112

Hepatoprotective activity

132

Hyperalgesia

108

Immunoaffinity
 chromatography

 

94

Lipid peroxidation

112

Lipid peroxidation

132

Liver

112

Liver glycogen

132

Lycopene

108

Microfilariae

128

NFATg

89

Onychium contiguum

100

Oxidative stress

108

Party Smart

132

Pathology

100

Phytochemicals

128

Propolis extract

112

Pteridium aquilinum

100

Renal

89

Ricinus communis

128

SDS-PAGE

94

Statistical medium
 optimization

 

138

Streptomyces

138

Ton EBP

89

Vinblastin

112

Vitex negundo

128

 

 

 

 

Indian Journal of Experimental Biology

Vol 46, February 2008, pp. 89-93

 

 

 

Mini Review

 

 

Newer insights into renal regulation of water homeostasis

Manoj G Tyagi & J Nandhakumar

 

Department of Pharmacology, Christian Medical College, Vellore 632002, India

 

The regulation of high osmolality is an important driving force for water reabsorption and urinary concentrationthe key functions of the kidney for maintaining optimum body fluid volume. New evidence shows that transcription factor tonicity responsive enhancer binding protein (TonEBP) and calcineurin-nuclear factor of activated T cells through cross-talk enhance Aquaporin 2 (AQP2) expression. AQP2 is the predominant vasopressin regulated water channel of the kidney collecting duct and is essential for urinary concentration. The serine/threonine phosphatase calcineurin is an important signaling molecule involved in kidney development and function. One potential target of calcineurin action is the water channel AQP2. The nuclear factor of activated T cells (NFAT) family has recently been expanded by the discovery of a new member, NFAT 5, or Ton EBP. Ton EBP is the only known mammalian transcription factor that regulates gene expression in response to hypertonicity. This review examines the importance of AQP2, calcineurin, NFATc and TonEBP in the renal regulation of water homeostasis.

 

 

Papers

 

Indian Journal of Experimental Biology

Vol 46, February 2008, pp.94-99

 

 

Detection of antiHaemonchus contortus antibodies in sheep by dot- ELISA with immunoaffinity purified fraction of ES antigen during prepatency

A Prasad, A Nasir & Nem Singh

 

Received 3 April 2007; revised 20 November 2007

Haemonchosis is a very common disease in small ruminants caused by H. contortus, a blood sucking parasite causing anaemia that may be fatal particularly to young animals. Therefore, detection of the infection during prepatent period is important for early treatment. Excretory-Secretory (ES) protein of H. contortus was purified through immunoaffinity chromatography. Dot -ELISA was performed with crude ES antigen as well as immunoaffinity purified fraction (F-1) with experimental and natural sera of sheep infected with H. contortus. Solid dot formation took place with 4 day, 1, 2 and 3 weeks post infection sera. Dot formation did not take place with negative control serum and uninfected control animal serum. When crude ES antigens was reacted to natural sheep sera having H. contortus infection, 60% sera samples showed solid dot formation whereas in F-1 fraction 75% of the sera samples showed solid dot indicating purified fraction was a more potent antigen. Crude ES and F-1 were also fractionated through SDS-PAGE. ES antigen revealed polypeptides in the range of 10 to 200 kDa of which 26, 32, 60 and 120 kDa were found more prominent. F-1 fraction on SDS-PAGE analysis revealed only four polypeptides of 26, 32, 60, and 120 of which 60 and 120 kDa were found to be most prominent. Results indicate that the purified fraction of ES antigen may be utilized for early diagnosis of haemonchosis. Further studies on cross antigenicity of this fraction with other nematode and trematode needs to be conducted.

 

 

Indian Journal of Experimental Biology

Vol 46, February 2008, pp.100-107

 

 

Pathological studies on Bovine papilloma virus-fern interaction in hamsters

 

G D Leishangthem, R Somvanshi & D R Lauren

 

Received 3 April 2007; revised 30 November 2007

Early pathological changes of Bovine papilloma virus (BPV-2)-fern (Pteridium aquilinum and Onychium contiguum fern) interaction in hamsters were studied. In bracken-exposed cattle, BPV induces malignancy in gastrointestinal and urinary bladder mucosa. Cutaneous warts were transmitted successfully in hamsters approximately after 3 months post inoculation while urinary bladder tumour of enzootic bovine haematuria cases were not transmitted. Histologically, tumour was diagnosed as fibroma. Onychium produced more pronounced effects than bracken fern which was characterized by significant reduction in body weight and testicular atrophy. BPV-fern interaction was not appreciable during early period of tumour induction and requires long-term studies for 12 to 18 months.

 

 

Indian Journal of Experimental Biology

Vol 46, February 2008, pp.108-111

 

 

Lycopene ameliorates thermal hyperalgesia and cold allodynia in STZ-induced diabetic rat

Anurag Kuhad & Kanwaljit Chopra

 

Received 25 May 2007; 20 revised November 2007

Peripheral neuropathy is one of the common complications of diabetes mellitus. It is frequently associated with debilitating pain. The present study was designed to investigate effect of Lycopene, a carotenoid found in tomatoes, on hyperalgesia and cold allodynia in streptozotocin (STZ) induced diabetic rats. After 4-weeks of STZ injection, diabetic mice exhibited a significant thermal hyperalgesia cold allodynia, hyperglycemia and loss of body weights as compared with control rats. Chronic treatment of lycopene for 4 weeks significantly attenuated the cold allodynia and thermal hyperalgesia. The results emphasize the role of antioxidant such as lycopene as an adjuvant therapy in the treatment of diabetic neuropathy.

 

 

Indian Journal of Experimental Biology

Vol 46, February 2008, pp.112-119

 

 

Efficiency of propolis extract against mitochondrial stress induced by antineoplasic agents (doxorubicin and vinblastin) in rats

Lamia Benguedouar, Hanane Nadia Boussenane, Wided Kebsa,
Mohamed Alyane, Hassiba Rouibah & Mesbah Lahouel

 

Received 09 April 2007 ; revised 06 November 2007

To assess the oxidative stress and mitochondrial dysfunction associated with disease, toxic process and aging, in vivo and in vitro preventive effect of propolis extract against mitochondrial oxidative stress induced by two anticancer drugs (doxorubicin and vinblastin) have been investigated in female wistar rat using liver and heart mitochondria. The results show that doxorubicin and vinblastin altered mitochondrial functions as observed by a decrease in respiratory control value, an activation of swelling and overproduction of superoxide anion. Myocardial tissue from doxorubicin treated rats showed a marked increase in malondialdehyde production, a depletion of reduced glutathione contents and an inhibition of catalase and superoxide dismutase activities. Similar results were also observed in liver tissue. Pretreatment of rats with propolis extract (100 mg/kg/day po) (10-4 M ip) administered 4 days prior to doxorubicin (20 mg/kg) and/or vinblastin (2 mg/kg) injection, substantially reduced the peroxidative damage in myocardium and hepatic tissues and markedly restored the tissues catalase and SOD activities. The results strongly suggest that propolis extract protects heart and liver tissues from oxidative stress by protecting the mitochondria.

 

 

 

Indian Journal of Experimental Biology

Vol 46, February 2008, pp.120-127

 

 

In vitro antiviral activity of plant extracts on goatpox virus replication

V Bhanuprakash, M Hosamani, V Balamurugan, P Gandhale, Ram Naresh, D Swarup & R K Singh

 

Received 16 April 2007; revised 11 December 2007

Four plants having known medicinal properties were screened for inhibition of goatpox virus (GTPV) replication in vitro. Of the 4 plants, extract of Acacia arabica (Babul) and Eugenia jambolana (Jamun) leaves had inhibition (%) 99.70 and 99.92 at their maximum non toxic concentrations, 99.93ア0.38 and 1999.73ア0.50 mg/ml, respectively in all cytopathic effect (CPE) inhibition assays. Inhibition of GTPV virus replication was further confirmed by PCR and SYBR Green based quantitative real-time QPCR assays specific for GTPV. Results indicated that the extract of Acacia arabica and Eugenia jambolana leaves inhibited GTPV replication in vitro.

 

 

 

Indian Journal of Experimental Biology

Vol 46, February 2008, pp.128-131

 

 

Anti-microfilarial activity of methanolic extract of Vitex negundo and
Aegle marmelos and their phytochemical analysis

K N Sahare, V Anandhraman, V G Meshram, S U Meshram, M V R Reddy, P M Tumane & K Goswami

 

Received 13 April 2007; revised 6 November 2007

In the present study, methanolic extracts of roots of Vitex negundo L. and extracts of leaves of Vitex negundo L., Ricinus communis L. and Aegle marmelos Corr. were explored for possible antifilarial effect against Brugia malayi microfilariae. It was observed that among the herbal extracts, root extract of Vitex negundo L. and leaves extract of Aegle marmelos Corr. at 100 ng/ml concentration showed complete loss of motility of microfilariae after 48 hr of incubation. Thin layer chromatography of the extracts revealed the presence of alkaloids, saponin and flavonoids in the roots of Vitex negundo L. and coumarin in the leaves of Aegle marmelos Corr.

 

 

 

Indian Journal of Experimental Biology

Vol 46, February 2008, pp.132-137

 

 

Ameliorative effect of PartySmart in rat model of alcoholic liver disease

S Gopumadhavan, Mohammed Rafiq, M Azeemuddin & S K Mitra

 

Received 8 August 2007; revised 23 November 2007

Present study was designed to investigate the effect of polyherbal formulation PartySmart in experimental model of alcoholic liver disease in male Wistar strain rats. Alcohol plus fish oil were administered to animals for 8 weeks to induce liver injury. PartySmart was administered at doses of 250 and 500 mg/kg body weight. After 8 weeks, parameters such as liver weight, liver function serum markers alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP) and lipid peroxidation were studied. Livers from all the groups were subjected for histological evaluation. Treatment with PartySmart at the dose of 500 mg/kg body weight showed significant reduction in the levels of serum ALT, AST and ALP with a decrease in liver weight as compared to ethanol-fed rats. A significant decrease was also observed in malondialdehyde levels following treatment with PartySmart at 500 mg/kg body weight. Histological profile of liver tissue in PartySmart-treated animals showed lesser vacuolar degeneration and intactness of hepatic architecture along with improved glycogen deposition as demonstrated by PAS staining. PartySmart ameliorated alcohol-induced liver injury by preventing cell membrane disturbances, reduction of oxidative stress by free radical scavenging and antioxidant activity and normalization of altered intracellular redox status. Thus, PartySmart can be beneficial in the treatment of alcohol-induced liver damage.

 

 

 

Indian Journal of Experimental Biology

Vol 46, February 2008, pp.138-144

 

 

Nutritional regulation of actinomycin-D production by a new isolate of Streptomyces sindenensis using statistical methods

Vandana Praveen, Divya Tripathi, C K M Tripathi & Vinod Bihari

 

Received 14 September 2007; revised 23 November 2007

Production of actinomycin-D, by an isolate, S. sindenensis, was optimized by statistical methods. Fructose peptone and NaNO3 were found to be critical for antibiotic production. In the second step, their concentrations were optimized with Central Composite Design and Response Surface Methodology. Fructose, peptone and NaNO3 at 2.55, 0.309 and 0.114 % respectively gave ~261 % higher yield (289 mg/l). Cultivation in fermentor at 600 rpm agitation and 1.5 vvm aeration with optimized medium gave 3.56 folds higher yield (365 mg/l) as compared to the yields in shake flasks using normal production medium (80 mg/l).